CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-031563
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ubiquitin carboxyl-terminal hydrolase 
Protein Synonyms/Alias
  
Gene Name
 USP28 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
118SAALDLLKGAFRSSEubiquitination[1, 2]
180TEGVREGKPFCNNETubiquitination[3]
682FFQNEAPKRVVERTLubiquitination[2]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094] 
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Hydrolase; Protease; Reference proteome; Thiol protease; Ubl conjugation pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 920 AA 
Protein Sequence
MHEATSAETK RSKRKRCEVW GENPNPNDWR RVDGWPVGLK NVGNTCWFSA VIQSLFQLPE 60
FRRLVLSYSL PQNVLENCRS HTEKRNIMFM QELQYLFALM MGSNRKFVDP SAALDLLKGA 120
FRSSEEQQQD VSEFTHKLLD WLEDAFQLAV NVNSPRNKSE NPMVQLFYGT FLTEGVREGK 180
PFCNNETFGQ YPLQVNGYRN LDECLEGAMV EGDVELLPSD HSVKYGQERW FTKLPPVLTF 240
ELSRFEFNQS LGQPEKIHNK LEFPQIIYMD RYMYRSKELI RNKRECIRKL KEEIKILQQK 300
LERYVKYGSG PARFPLPDML KYVIEFASTK PASESCPPES DTHMTLPLSS VHCSVSDQTS 360
KESTSTESSS QDVESTFSSP EDSLPKSKPL TSSRSSMEMP SQPAPRTVTD EEINFVKTCL 420
QRWRSEIEQD IQDLKTCIAS TTQTIEQMYC DPLLRQVPYR LHAVLVHEGQ ANAGHYWAYI 480
YNQPRQSWLK YNDISVTESS WEEVERDSYG GLRNVSAYCL MYINDKLPYF NAEAAPTESD 540
QMSEVEALSV ELKHYIQEDN WRFEQEVEEW EEEQSCKIPQ MESSTNSSSQ DYSTSQEPSV 600
ASSHGVRCLS SEHAVIVKEQ TAQAIANTAR AYEKSGVEAA LSEAFHEEYS RLYQLAKETP 660
TSHSDPRLQH VLVYFFQNEA PKRVVERTLL EQFADKNLSY DERSISIMKV AQAKLKEIGP 720
DDMNMEEYKK WHEDYSLFRK VSVYLLTGLE LYQKGKYQEA LSYLVYAYQS NAALLMKGPR 780
RGVKESVIAL YRRKCLLELN AKAASLFETN DDHSVTEGIN VMNELIIPCI HLIINNDISK 840
DDLDAIEVMR NHWCSYLGQD IAENLQLCLG EFLPRLLDPS AEIIVLKEPP TIRPNSPYDL 900
CSRFAAVMES IQGVSTVTVK 920 
Gene Ontology
 GO:0005730; C:nucleolus; IDA:HPA.
 GO:0008234; F:cysteine-type peptidase activity; IEA:UniProtKB-KW.
 GO:0004221; F:ubiquitin thiolesterase activity; IEA:InterPro.
 GO:0006511; P:ubiquitin-dependent protein catabolic process; IEA:InterPro. 
Interpro
 IPR018200; Pept_C19ubi-hydrolase_C_CS.
 IPR001394; Peptidase_C19. 
Pfam
 PF00443; UCH 
SMART
  
PROSITE
 PS00972; UCH_2_1
 PS00973; UCH_2_2
 PS50235; UCH_2_3 
PRINTS