UniProt Accession

 ATIF1_MOUSE; O35143; Q9D879 

Genbank Protein ID

 AF002718; AK008346; AK152389; AL805897; CH466552; BC012680 

Genbank Nucleotide ID

 AAB69647.1; BAB25618.1; BAE31177.1; CAM26517.1; EDL30101.1; AAH12680.1 

Protein Name

 ATPase inhibitor, mitochondrial 

Protein Synonyms/Alias

 Inhibitor of F(1)F(o)-ATPase; IF(1); IF1 

Gene Name

 Atpif1 

Gene Synonyms/Alias

 Atpi; If1 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
49	EAGGAFGKREKAEED	acetylation	[1]
52	GAFGKREKAEEDRYF	acetylation	[1]
64	RYFREKTKEQLAALR	acetylation	[1]
64	RYFREKTKEQLAALR	ubiquitination	[2]
90	KEIERLQKQIERHKK	acetylation	[1]

Reference

 [1] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Endogenous F(1)F(o)-ATPase inhibitor limiting ATP depletion when the mitochondrial membrane potential falls below a threshold and the F(1)F(o)-ATP synthase starts hydrolyzing ATP to pump protons out of the mitochondrial matrix. Required to avoid the consumption of cellular ATP when the F(1)F(o)-ATP synthase enzyme acts as an ATP hydrolase (By similarity). Indirectly acts as a regulator of heme synthesis in erythroid tissues: regulates heme synthesis by modulating the mitochondrial pH and redox potential, allowing FECH to efficiently catalyze the incorporation of iron into protoporphyrin IX to produce heme. 

Sequence Annotation

 REGION 26 52 N-terminal inhibitory region (By
 REGION 74 106 Antiparallel alpha-helical coiled coil  

Keyword

 Coiled coil; Complete proteome; Mitochondrion; Reference proteome; Transit peptide. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 106 AA 

Protein Sequence

Gene Ontology

 GO:0009986; C:cell surface; ISS:UniProtKB.
 GO:0005753; C:mitochondrial proton-transporting ATP synthase complex; IEA:Compara.
 GO:0005739; C:mitochondrion; ISS:UniProtKB.
 GO:0043532; F:angiostatin binding; ISS:UniProtKB.
 GO:0051117; F:ATPase binding; ISS:UniProtKB.
 GO:0042030; F:ATPase inhibitor activity; ISS:UniProtKB.
 GO:0005516; F:calmodulin binding; ISS:UniProtKB.
 GO:0042803; F:protein homodimerization activity; ISS:UniProtKB.
 GO:0030218; P:erythrocyte differentiation; IMP:UniProtKB.
 GO:0006783; P:heme biosynthetic process; IMP:UniProtKB.
 GO:0001937; P:negative regulation of endothelial cell proliferation; ISS:UniProtKB.
 GO:0051346; P:negative regulation of hydrolase activity; ISS:UniProtKB.
 GO:0045980; P:negative regulation of nucleotide metabolic process; IEA:InterPro.
 GO:0051289; P:protein homotetramerization; ISS:UniProtKB.
 GO:0072593; P:reactive oxygen species metabolic process; IMP:MGI.
 GO:0042981; P:regulation of apoptotic process; IMP:MGI. 

Interpro

 IPR007648; ATPase_inhibitor_IATP_mt. 

Pfam

 PF04568; IATP 

SMART

  

PROSITE

  

PRINTS