UniProt Accession

 NTAQ1_HUMAN; Q96HA8; Q9NW95 

Genbank Protein ID

 AK001066; BC008781 

Genbank Nucleotide ID

 BAA91488.1; AAH08781.1 

Protein Name

 Protein N-terminal glutamine amidohydrolase 

Protein Synonyms/Alias

 Protein NH2-terminal glutamine deamidase; N-terminal Gln amidase; Nt(Q)-amidase; WDYHV motif-containing protein 1 

Gene Name

 WDYHV1 

Gene Synonyms/Alias

 C8orf32; NTAQ1 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
140	IRADSYLKNFASDRS	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 Mediates the side-chain deamidation of N-terminal glutamine residues to glutamate, an important step in N-end rule pathway of protein degradation. Conversion of the resulting N- terminal glutamine to glutamate renders the protein susceptible to arginylation, polyubiquitination and degradation as specified by the N-end rule. Does not act on substrates with internal or C- terminal glutamine and does not act on non-glutamine residues in any position. Does not deaminate acetylated N-terminal glutamine. With the exception of proline, all tested second-position residues on substrate peptides do not greatly influence the activity. In contrast, a proline at position 2, virtually abolishes deamidation of N-terminal glutamine (By similarity). 

Sequence Annotation

 ACT_SITE 28 28 By similarity.
 ACT_SITE 81 81 By similarity.
 ACT_SITE 97 97 By similarity.  

Keyword

 3D-structure; Complete proteome; Cytoplasm; Hydrolase; Nucleus; Polymorphism; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 205 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; ISS:UniProtKB.
 GO:0005634; C:nucleus; ISS:UniProtKB.
 GO:0070773; F:protein-N-terminal glutamine amidohydrolase activity; ISS:UniProtKB.
 GO:0006464; P:cellular protein modification process; ISS:UniProtKB. 

Interpro

 IPR023128; Prot_N_Gln_amidohydro_ab_roll. 

Pfam

 PF09764; Nt_Gln_amidase 

SMART

  

PROSITE

  

PRINTS