CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000993
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 85/88 kDa calcium-independent phospholipase A2 
Protein Synonyms/Alias
 CaI-PLA2; Group VI phospholipase A2; GVI PLA2; Intracellular membrane-associated calcium-independent phospholipase A2 beta; iPLA2-beta; Patatin-like phospholipase domain-containing protein 9; PNPLA9 
Gene Name
 PLA2G6 
Gene Synonyms/Alias
 PLPLA9 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
25FSNPFRVKEVAVADYubiquitination[1]
295ASPLHWAKNAEMARMubiquitination[1]
573REFGEHTKMTDVRKPubiquitination[1]
719SNPWELAKTVFGAKEubiquitination[1]
725AKTVFGAKELGKMVVubiquitination[1]
729FGAKELGKMVVDCCTubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Catalyzes the release of fatty acids from phospholipids. It has been implicated in normal phospholipid remodeling, nitric oxide-induced or vasopressin-induced arachidonic acid release and in leukotriene and prostaglandin production. May participate in fas mediated apoptosis and in regulating transmembrane ion flux in glucose-stimulated B-cells. Has a role in cardiolipin (CL) deacylation. Required for both speed and directionality of monocyte MCP1/CCL2-induced chemotaxis through regulation of F- actin polymerization at the pseudopods. 
Sequence Annotation
 REPEAT 151 181 ANK 1.
 REPEAT 185 215 ANK 2.
 REPEAT 219 248 ANK 3.
 REPEAT 251 281 ANK 4.
 REPEAT 286 312 ANK 5.
 REPEAT 316 345 ANK 6.
 REPEAT 349 378 ANK 7.
 DOMAIN 481 665 Patatin.
 REGION 677 696 Calmodulin-binding (By similarity).
 REGION 748 759 Calmodulin-binding (By similarity).
 ACT_SITE 519 519 Potential.
 MOD_RES 587 587 Phosphothreonine (By similarity).  
Keyword
 Alternative splicing; ANK repeat; Calmodulin-binding; Chemotaxis; Complete proteome; Cytoplasm; Disease mutation; Dystonia; Hydrolase; Lipid degradation; Lipid metabolism; Membrane; Neurodegeneration; Parkinsonism; Pharmaceutical; Phosphoprotein; Polymorphism; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 806 AA 
Protein Sequence
MQFFGRLVNT FSGVTNLFSN PFRVKEVAVA DYTSSDRVRE EGQLILFQNT PNRTWDCVLV 60
NPRNSQSGFR LFQLELEADA LVNFHQYSSQ LLPFYESSPQ VLHTEVLQHL TDLIRNHPSW 120
SVAHLAVELG IRECFHHSRI ISCANCAENE EGCTPLHLAC RKGDGEILVE LVQYCHTQMD 180
VTDYKGETVF HYAVQGDNSQ VLQLLGRNAV AGLNQVNNQG LTPLHLACQL GKQEMVRVLL 240
LCNARCNIMG PNGYPIHSAM KFSQKGCAEM IISMDSSQIH SKDPRYGASP LHWAKNAEMA 300
RMLLKRGCNV NSTSSAGNTA LHVAVMRNRF DCAIVLLTHG ANADARGEHG NTPLHLAMSK 360
DNVEMIKALI VFGAEVDTPN DFGETPTFLA SKIGRLVTRK AILTLLRTVG AEYCFPPIHG 420
VPAEQGSAAP HHPFSLERAQ PPPISLNNLE LQDLMHISRA RKPAFILGSM RDEKRTHDHL 480
LCLDGGGVKG LIIIQLLIAI EKASGVATKD LFDWVAGTST GGILALAILH SKSMAYMRGM 540
YFRMKDEVFR GSRPYESGPL EEFLKREFGE HTKMTDVRKP KVMLTGTLSD RQPAELHLFR 600
NYDAPETVRE PRFNQNVNLR PPAQPSDQLV WRAARSSGAA PTYFRPNGRF LDGGLLANNP 660
TLDAMTEIHE YNQDLIRKGQ ANKVKKLSIV VSLGTGRSPQ VPVTCVDVFR PSNPWELAKT 720
VFGAKELGKM VVDCCTDPDG RAVDRARAWC EMVGIQYFRL NPQLGTDIML DEVSDTVLVN 780
ALWETEVYIY EHREEFQKLI QLLLSP 806 
Gene Ontology
 GO:0005813; C:centrosome; IDA:HPA.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
 GO:0005739; C:mitochondrion; IEA:Compara.
 GO:0047499; F:calcium-independent phospholipase A2 activity; IEA:Compara.
 GO:0004623; F:phospholipase A2 activity; TAS:ProtInc.
 GO:0035965; P:cardiolipin acyl-chain remodeling; TAS:Reactome.
 GO:0032049; P:cardiolipin biosynthetic process; IMP:UniProtKB.
 GO:0008219; P:cell death; IEA:UniProtKB-KW.
 GO:0006935; P:chemotaxis; IEA:UniProtKB-KW.
 GO:0007204; P:elevation of cytosolic calcium ion concentration; IEA:Compara.
 GO:0038096; P:Fc-gamma receptor signaling pathway involved in phagocytosis; TAS:Reactome.
 GO:0045087; P:innate immune response; TAS:Reactome.
 GO:0016042; P:lipid catabolic process; IEA:UniProtKB-KW.
 GO:0060135; P:maternal process involved in female pregnancy; IEA:Compara.
 GO:0007613; P:memory; IEA:Compara.
 GO:0051967; P:negative regulation of synaptic transmission, glutamatergic; IEA:Compara.
 GO:0036151; P:phosphatidylcholine acyl-chain remodeling; TAS:Reactome.
 GO:0036152; P:phosphatidylethanolamine acyl-chain remodeling; TAS:Reactome.
 GO:0090238; P:positive regulation of arachidonic acid secretion; IEA:Compara.
 GO:2000304; P:positive regulation of ceramide biosynthetic process; IEA:Compara.
 GO:0045921; P:positive regulation of exocytosis; IEA:Compara.
 GO:0035774; P:positive regulation of insulin secretion involved in cellular response to glucose stimulus; IEA:Compara.
 GO:0090037; P:positive regulation of protein kinase C signaling cascade; IEA:Compara.
 GO:0001934; P:positive regulation of protein phosphorylation; IEA:Compara.
 GO:0090200; P:positive regulation of release of cytochrome c from mitochondria; IEA:Compara.
 GO:0045909; P:positive regulation of vasodilation; IEA:Compara.
 GO:1901339; P:regulation of store-operated calcium channel activity; IEA:Compara.
 GO:0034976; P:response to endoplasmic reticulum stress; IEA:Compara.
 GO:0014832; P:urinary bladder smooth muscle contraction; IEA:Compara. 
Interpro
 IPR016035; Acyl_Trfase/lysoPLipase.
 IPR002110; Ankyrin_rpt.
 IPR020683; Ankyrin_rpt-contain_dom.
 IPR002641; Patatin/PLipase_A2-rel. 
Pfam
 PF00023; Ank
 PF01734; Patatin 
SMART
 SM00248; ANK 
PROSITE
 PS50297; ANK_REP_REGION
 PS50088; ANK_REPEAT 
PRINTS
 PR01415; ANKYRIN.