CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-011899
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Nuclear pore complex protein Nup160 
Protein Synonyms/Alias
 160 kDa nucleoporin; Nucleoporin Nup160 
Gene Name
 NUP160 
Gene Synonyms/Alias
 KIAA0197; NUP120 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
93FYYVESGKLFSVTRNubiquitination[1]
106RNRFIHWKTSGDTLEacetylation[2]
131LNNAIRLKFQNCSVLubiquitination[1, 3]
258MVSVVELKQSSVMQRubiquitination[1, 4, 5]
343LTAGTGHKLRLAYSPubiquitination[1]
495DLSWSELKKEVTLAVubiquitination[1, 3, 4, 5]
496LSWSELKKEVTLAVEubiquitination[1]
608RDVICLIKCLRLIEEubiquitination[3]
689ETEVEMEKGFNPAQPubiquitination[1, 3, 4, 5]
722IVCRGVHKIASTRFLubiquitination[4]
833FFQEVARKHIISHLFubiquitination[1]
925LVTGEGQKALECFCQubiquitination[1, 3]
939QAASEVGKEEFLDRLubiquitination[1, 3]
965PRLQYYDKVLRLLDVubiquitination[1, 3, 4, 5, 6, 7, 8, 9]
1006TLRTCIFKHHLDLGHubiquitination[1]
1096IYRHNYRKAGTVMFEubiquitination[1, 4]
1120RTLRGLEKQGNCYLAubiquitination[1, 3, 7]
1330NWLINSYKKVDAAELubiquitination[10]
1415LSQKILDKLEDYQQKubiquitination[1, 4, 5, 6, 8, 9, 10]
1422KLEDYQQKVDKATRDubiquitination[1, 4, 10, 11]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786]
 [3] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [6] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [7] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [8] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [9] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [10] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [11] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 Involved in poly(A)+ RNA transport. 
Sequence Annotation
 MOD_RES 1157 1157 Phosphoserine.
 MOD_RES 1195 1195 Phosphothreonine (By similarity).  
Keyword
 Alternative splicing; Complete proteome; mRNA transport; Nuclear pore complex; Nucleus; Phosphoprotein; Polymorphism; Protein transport; Reference proteome; Translocation; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1436 AA 
Protein Sequence
MLHLSAAPPA PPPEVTATAR PCLCSVGRRG DGGKMAAAGA LERSFVELSG AERERPRHFR 60
EFTVCSIGTA NAVAGAVKYS ESAGGFYYVE SGKLFSVTRN RFIHWKTSGD TLELMEESLD 120
INLLNNAIRL KFQNCSVLPG GVYVSETQNR VIILMLTNQT VHRLLLPHPS RMYRSELVVD 180
SQMQSIFTDI GKVDFTDPCN YQLIPAVPGI SPNSTASTAW LSSDGEALFA LPCASGGIFV 240
LKLPPYDIPG MVSVVELKQS SVMQRLLTGW MPTAIRGDQS PSDRPLSLAV HCVEHDAFIF 300
ALCQDHKLRM WSYKEQMCLM VADMLEYVPV KKDLRLTAGT GHKLRLAYSP TMGLYLGIYM 360
HAPKRGQFCI FQLVSTESNR YSLDHISSLF TSQETLIDFA LTSTDIWALW HDAENQTVVK 420
YINFEHNVAG QWNPVFMQPL PEEEIVIRDD QDPREMYLQS LFTPGQFTNE ALCKALQIFC 480
RGTERNLDLS WSELKKEVTL AVENELQGSV TEYEFSQEEF RNLQQEFWCK FYACCLQYQE 540
ALSHPLALHL NPHTNMVCLL KKGYLSFLIP SSLVDHLYLL PYENLLTEDE TTISDDVDIA 600
RDVICLIKCL RLIEESVTVD MSVIMEMSCY NLQSPEKAAE QILEDMITID VENVMEDICS 660
KLQEIRNPIH AIGLLIREMD YETEVEMEKG FNPAQPLNIR MNLTQLYGSN TAGYIVCRGV 720
HKIASTRFLI CRDLLILQQL LMRLGDAVIW GTGQLFQAQQ DLLHRTAPLL LSYYLIKWGS 780
ECLATDVPLD TLESNLQHLS VLELTDSGAL MANRFVSSPQ TIVELFFQEV ARKHIISHLF 840
SQPKAPLSQT GLNWPEMITA ITSYLLQLLW PSNPGCLFLE CLMGNCQYVQ LQDYIQLLHP 900
WCQVNVGSCR FMLGRCYLVT GEGQKALECF CQAASEVGKE EFLDRLIRSE DGEIVSTPRL 960
QYYDKVLRLL DVIGLPELVI QLATSAITEA GDDWKSQATL RTCIFKHHLD LGHNSQAYEA 1020
LTQIPDSSRQ LDCLRQLVVV LCERSQLQDL VEFPYVNLHN EVVGIIESRA RAVDLMTHNY 1080
YELLYAFHIY RHNYRKAGTV MFEYGMRLGR EVRTLRGLEK QGNCYLAALN CLRLIRPEYA 1140
WIVQPVSGAV YDRPGASPKR NHDGECTAAP TNRQIEILEL EDLEKECSLA RIRLTLAQHD 1200
PSAVAVAGSS SAEEMVTLLV QAGLFDTAIS LCQTFKLPLT PVFEGLAFKC IKLQFGGEAA 1260
QAEAWAWLAA NQLSSVITTK ESSATDEAWR LLSTYLERYK VQNNLYHHCV INKLLSHGVP 1320
LPNWLINSYK KVDAAELLRL YLNYDLLEEA VDLVSEYVDA VLGKGHQYFG IEFPLSATAP 1380
MVWLPYSSID QLLQALGENS ANSHNIALSQ KILDKLEDYQ QKVDKATRDL LYRRTL 1436 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0031080; C:nuclear pore outer ring; IDA:UniProtKB.
 GO:0005487; F:nucleocytoplasmic transporter activity; IDA:UniProtKB.
 GO:0005975; P:carbohydrate metabolic process; TAS:Reactome.
 GO:0019221; P:cytokine-mediated signaling pathway; TAS:Reactome.
 GO:0015758; P:glucose transport; TAS:Reactome.
 GO:0000278; P:mitotic cell cycle; TAS:Reactome.
 GO:0006406; P:mRNA export from nucleus; IDA:UniProtKB.
 GO:0015031; P:protein transport; IEA:UniProtKB-KW.
 GO:0010827; P:regulation of glucose transport; TAS:Reactome.
 GO:0044281; P:small molecule metabolic process; TAS:Reactome.
 GO:0055085; P:transmembrane transport; TAS:Reactome.
 GO:0022415; P:viral reproductive process; TAS:Reactome. 
Interpro
 IPR021717; Nucleoporin_Nup160. 
Pfam
 PF11715; Nup160 
SMART
  
PROSITE
  
PRINTS