UniProt Accession

 HNRH2_HUMAN; P55795; A1L400; Q9HHA7 

Genbank Protein ID

 U01923; U78027; AL035422; CH471115; BC130343; BC130345 

Genbank Nucleotide ID

 AAB64202.1; CAB55879.2; EAX02864.1; AAI30344.1; AAI30346.1 

Protein Name

 Heterogeneous nuclear ribonucleoprotein H2 

Protein Synonyms/Alias

 hnRNP H2; FTP-3; Heterogeneous nuclear ribonucleoprotein H'; hnRNP H'; Heterogeneous nuclear ribonucleoprotein H2, N-terminally processed 

Gene Name

 HNRNPH2 

Gene Synonyms/Alias

 FTP3; HNRPH2 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
14	GREGFVVKVRGLPWS	ubiquitination	[1]
35	MRFFSDCKIQNGTSG	ubiquitination	[1, 2, 3, 4, 5, 6, 7, 8]
73	EVKLALKKDRETMGH	ubiquitination	[1]
98	VEMDWVLKHTGPNSP	methylation	[9]
98	VEMDWVLKHTGPNSP	ubiquitination	[1, 4, 6]
167	ASQEIAEKALKKHKE	ubiquitination	[1, 7]
171	IAEKALKKHKERIGH	ubiquitination	[1]
185	HRYIEIFKSSRAEVR	ubiquitination	[1, 7]
200	THYDPPRKLMAMQRP	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [5] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [6] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [7] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [8] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [9] Large-scale global identification of protein lysine methylation in vivo.
 Cao XJ, Arnaudo AM, Garcia BA.
 Epigenetics. 2013 May 1;8(5):477-85. [PMID: 23644510] 

Functional Description

 This protein is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Binds poly(RG). 

Sequence Annotation

 DOMAIN 11 90 RRM 1.
 DOMAIN 111 188 RRM 2.
 REPEAT 234 249 1-1.
 DOMAIN 289 364 RRM 3.
 REPEAT 354 372 2-1.
 REPEAT 374 392 2-2.
 REPEAT 418 433 1-2.
 REGION 234 433 2 X 16 AA Gly-rich approximate repeats.
 REGION 354 392 2 X 19 AA perfect repeats.
 MOD_RES 1 1 N-acetylmethionine.
 MOD_RES 2 2 N-acetylmethionine; in Heterogeneous
 MOD_RES 23 23 Phosphoserine (By similarity).
 MOD_RES 63 63 Phosphoserine (By similarity).
 MOD_RES 104 104 Phosphoserine (By similarity).
 MOD_RES 107 107 Phosphothreonine (By similarity).
 MOD_RES 233 233 Dimethylated arginine; alternate (By
 MOD_RES 233 233 Omega-N-methylarginine; alternate (By
 MOD_RES 246 246 Phosphotyrosine (By similarity).
 MOD_RES 310 310 Phosphoserine.  

Keyword

 3D-structure; Acetylation; Complete proteome; Direct protein sequencing; Methylation; Nucleus; Phosphoprotein; Reference proteome; Repeat; Ribonucleoprotein; RNA-binding. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 449 AA 

Protein Sequence

Gene Ontology

 GO:0015629; C:actin cytoskeleton; IDA:HPA.
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0030529; C:ribonucleoprotein complex; IDA:MGI.
 GO:0000166; F:nucleotide binding; IEA:InterPro.
 GO:0003723; F:RNA binding; TAS:ProtInc.
 GO:0000398; P:mRNA splicing, via spliceosome; TAS:Reactome. 

Interpro

 IPR012677; Nucleotide-bd_a/b_plait.
 IPR000504; RRM_dom.
 IPR012996; Znf_CHHC. 

Pfam

 PF08080; zf-RNPHF 

SMART

 SM00360; RRM 

PROSITE

 PS50102; RRM 

PRINTS