UniProt Accession

 KTNB1_HUMAN; Q9BVA0; A6NCG6; O60620 

Genbank Protein ID

 AF052432; BT007022; CR456762; AC092118; CH471092; BC001353 

Genbank Nucleotide ID

 AAC09328.1; AAP35668.1; CAG33043.1; EAW82951.1; AAH01353.1 

Protein Name

 Katanin p80 WD40 repeat-containing subunit B1 

Protein Synonyms/Alias

 Katanin p80 subunit B1; p80 katanin 

Gene Name

 KATNB1 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
8	MATPVVTKTAWKLQE	ubiquitination	[1]
96	VWDLEAAKILRTLMG	ubiquitination	[1, 2, 3]
565	TVLPQIEKLLQSKYE	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 

Functional Description

 Participates in a complex which severs microtubules in an ATP-dependent manner. May act to target the enzymatic subunit of this complex to sites of action such as the centrosome. Microtubule severing may promote rapid reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation. Microtubule release from the mitotic spindle poles may allow depolymerization of the microtubule end proximal to the spindle pole, leading to poleward microtubule flux and poleward motion of chromosome. Microtubule release within the cell body of neurons may be required for their transport into neuronal processes by microtubule-dependent motor proteins. This transport is required for axonal growth (By similarity). 

Sequence Annotation

 REPEAT 18 58 WD 1.
 REPEAT 61 100 WD 2.
 REPEAT 103 142 WD 3.
 REPEAT 145 184 WD 4.
 REPEAT 187 226 WD 5.
 REPEAT 229 269 WD 6.
 REGION 1 300 Interaction with centrosomes.
 REGION 1 284 Interaction with dynein (By similarity).
 REGION 285 434 Interaction with PAFAH1B1 (By
 REGION 433 655 Interaction with KATNA1 and NDEL1 (By  

Keyword

 Cell cycle; Cell division; Complete proteome; Cytoplasm; Cytoskeleton; Microtubule; Mitosis; Polymorphism; Reference proteome; Repeat; WD repeat. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 655 AA 

Protein Sequence

Gene Ontology

 GO:0030424; C:axon; IEA:Compara.
 GO:0005813; C:centrosome; IEA:Compara.
 GO:0030426; C:growth cone; IEA:Compara.
 GO:0008352; C:katanin complex; TAS:UniProtKB.
 GO:0005874; C:microtubule; IEA:UniProtKB-KW.
 GO:0030496; C:midbody; IEA:Compara.
 GO:0043025; C:neuronal cell body; IEA:Compara.
 GO:0005886; C:plasma membrane; IDA:HPA.
 GO:0000922; C:spindle pole; IEA:UniProtKB-SubCell.
 GO:0008017; F:microtubule binding; NAS:UniProtKB.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0051013; P:microtubule severing; IEA:Compara.
 GO:0007067; P:mitosis; IEA:UniProtKB-KW.
 GO:0007026; P:negative regulation of microtubule depolymerization; IMP:UniProtKB.
 GO:0010942; P:positive regulation of cell death; IEA:Compara.
 GO:0031117; P:positive regulation of microtubule depolymerization; IMP:UniProtKB.
 GO:0010976; P:positive regulation of neuron projection development; IEA:Compara.
 GO:0006605; P:protein targeting; NAS:UniProtKB. 

Interpro

 IPR020472; G-protein_beta_WD-40_rep.
 IPR026962; Katanin_bsu1.
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR019775; WD40_repeat_CS.
 IPR017986; WD40_repeat_dom. 

Pfam

 PF00400; WD40 

SMART

 SM00320; WD40 

PROSITE

 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 

PRINTS

 PR00320; GPROTEINBRPT.