CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-015644
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Mediator of RNA polymerase II transcription subunit 25 
Protein Synonyms/Alias
 Activator interaction domain-containing protein 1; Activator-recruited cofactor 92 kDa component; ARC92; Mediator complex subunit 25; p78 
Gene Name
 MED25 
Gene Synonyms/Alias
 ACID1; ARC92; PTOV2; TCBAP0758 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
197ALRLLFEKAAPPALLubiquitination[1, 2]
484NKDLESLKGLYRIMGubiquitination[3, 4]
551HKQVQQQKLEQQQRGubiquitination[2]
Reference
 [1] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for RARA/RXRA-mediated transcription. 
Sequence Annotation
 REGION 1 226 Interaction with the Mediator complex.
 REGION 389 543 Interaction with VP16.
 REGION 395 545 Interaction with CREBBP.
 REGION 564 653 Interaction with RARA.
 REGION 640 707 Interaction with RARA.
 MOTIF 646 650 LXXLL motif.  
Keyword
 3D-structure; Activator; Alternative splicing; Charcot-Marie-Tooth disease; Complete proteome; Direct protein sequencing; Disease mutation; Neuropathy; Nucleus; Reference proteome; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 747 AA 
Protein Sequence
MVPGSEGPAR AGSVVADVVF VIEGTANLGP YFEGLRKHYL LPAIEYFNGG PPAETDFGGD 60
YGGTQYSLVV FNTVDCAPES YVQCHAPTSS AYEFVTWLDG IKFMGGGGES CSLIAEGLST 120
ALQLFDDFKK MREQIGQTHR VCLLICNSPP YLLPAVESTT YSGCTTENLV QQIGERGIHF 180
SIVSPRKLPA LRLLFEKAAP PALLEPLQPP TDVSQDPRHM VLVRGLVLPV GGGSAPGPLQ 240
SKQPVPLPPA APSGATLSAA PQQPLPPVPP QYQVPGNLSA AQVAAQNAVE AAKNQKAGLG 300
PRFSPITPLQ QAAPGVGPPF SQAPAPQLPP GPPGAPKPPP ASQPSLVSTV APGSGLAPTA 360
QPGAPSMAGT VAPGGVSGPS PAQLGAPALG GQQSVSNKLL AWSGVLEWQE KPKPASVDAN 420
TKLTRSLPCQ VYVNHGENLK TEQWPQKLIM QLIPQQLLTT LGPLFRNSRM VQFHFTNKDL 480
ESLKGLYRIM GNGFAGCVHF PHTAPCEVRV LMLLYSSKKK IFMGLIPYDQ SGFVNGIRQV 540
ITNHKQVQQQ KLEQQQRGMG GQQAPPGLGP ILEDQARPSQ NLLQLRPPQP QPQGTVGASG 600
ATGQPQPQGT AQPPPGAPQG PPGAASGPPP PGPILRPQNP GANPQLRSLL LNPPPPQTGV 660
PPPQASLHHL QPPGAPALLP PPHQGLGQPQ LGPPLLHPPP PATRGSCAAA SAAPGPGAAP 720
VGAPTPASTT CPVLARTTSP SGSTASGKTK ERRRGPCVSR KMGASLFLCG SEEHAYVFNM 780
QTNRVCVERI QPETPL 796 
Gene Ontology
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0048147; P:negative regulation of fibroblast proliferation; IMP:UniProtKB.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IMP:UniProtKB.
 GO:0071158; P:positive regulation of cell cycle arrest; IMP:UniProtKB.
 GO:0035563; P:positive regulation of chromatin binding; IMP:UniProtKB.
 GO:2001178; P:positive regulation of mediator complex assembly; IMP:UniProtKB.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IDA:UniProtKB.
 GO:0006367; P:transcription initiation from RNA polymerase II promoter; TAS:Reactome. 
Interpro
 IPR021394; Mediator_Med25.
 IPR021406; Mediator_Med25_NR-box.
 IPR021397; Mediator_Med25_SD1.
 IPR021419; Mediator_Med25_VWA. 
Pfam
 PF11232; Med25
 PF11244; Med25_NR-box
 PF11235; Med25_SD1
 PF11265; Med25_VWA 
SMART
  
PROSITE
  
PRINTS