CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022383
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 U3 small nucleolar RNA-associated protein 6 homolog 
Protein Synonyms/Alias
 Hepatocellular carcinoma-associated antigen 66; Multiple hat domains protein 
Gene Name
 UTP6 
Gene Synonyms/Alias
 C17orf40; HCA66; MHAT 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
43KASDLEYKIQRRTLFubiquitination[1]
80TRIGYSFKKDEIENSubiquitination[1]
81RIGYSFKKDEIENSIubiquitination[1, 2, 3]
177RFHPECPKLYKEYFRubiquitination[1]
180PECPKLYKEYFRMELubiquitination[1, 2, 3, 4, 5, 6]
192MELMHAEKLRKEKEEubiquitination[1]
197AEKLRKEKEEFEKASubiquitination[2]
295TEEQPTTKQAKAVEVubiquitination[1, 2, 5, 7]
298QPTTKQAKAVEVGRKubiquitination[1]
463DTEAVFKKALLAVIGubiquitination[1, 4, 6]
479DSVTLKNKYLDWAYRubiquitination[1, 4, 6]
498KKARAVFKSLQESRPubiquitination[1, 4, 6]
513FSVDFFRKMIQFEKEubiquitination[1]
519RKMIQFEKEQESCNMubiquitination[1]
575QIYWRAMKMLQGESAubiquitination[1]
588SAEAFVAKHAMHQTGubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [6] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [7] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094
Functional Description
 Involved in nucleolar processing of pre-18S ribosomal RNA (By similarity). 
Sequence Annotation
 REPEAT 121 153 HAT 1.
 REPEAT 156 188 HAT 2.
 REPEAT 304 335 HAT 3.
 REPEAT 488 520 HAT 4.
 REPEAT 524 557 HAT 5.  
Keyword
 Complete proteome; Nucleus; Polymorphism; Reference proteome; Repeat; rRNA processing. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 597 AA 
Protein Sequence
MAEIIQERIE DRLPELEQLE RIGLFSHAEI KAIIKKASDL EYKIQRRTLF KEDFINYVQY 60
EINLLELIQR RRTRIGYSFK KDEIENSIVH RVQGVFQRAS AKWKDDVQLW LSYVAFCKKW 120
ATKTRLSKVF SAMLAIHSNK PALWIMAAKW EMEDRLSSES ARQLFLRALR FHPECPKLYK 180
EYFRMELMHA EKLRKEKEEF EKASMDVENP DYSEEILKGE LAWIIYKNSV SIIKGAEFHV 240
SLLSIAQLFD FAKDLQKEIY DDLQALHTDD PLTWDYVARR ELEIESQTEE QPTTKQAKAV 300
EVGRKEERCC AVYEEAVKTL PTEAMWKCYI TFCLERFTKK SNSGFLRGKR LERTMTVFRK 360
AHELKLLSEC QYKQLSVSLL CYNFLREALE VAVAGTELFR DSGTMWQLKL QVLIESKSPD 420
IAMLFEEAFV HLKPQVCLPL WISWAEWSEG AKSQEDTEAV FKKALLAVIG ADSVTLKNKY 480
LDWAYRSGGY KKARAVFKSL QESRPFSVDF FRKMIQFEKE QESCNMANIR EYYERALREF 540
GSADSDLWMD YMKEELNHPL GRPENCGQIY WRAMKMLQGE SAEAFVAKHA MHQTGHL 597 
Gene Ontology
 GO:0005730; C:nucleolus; IDA:HPA.
 GO:0006364; P:rRNA processing; IEA:UniProtKB-KW. 
Interpro
 IPR003107; HAT.
 IPR011990; TPR-like_helical.
 IPR013949; U3_snoRNA_assoc-6. 
Pfam
 PF08640; U3_assoc_6 
SMART
 SM00386; HAT 
PROSITE
  
PRINTS