CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-019853
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 CLIP-associating protein 2 
Protein Synonyms/Alias
 Cytoplasmic linker-associated protein 2 
Gene Name
 Clasp2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
785QTPSLKVKVAILKYIubiquitination[1]
Reference
 [1] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113] 
Functional Description
 Microtubule plus-end tracking protein that promotes the stabilization of dynamic microtubules. Involved in the nucleation of noncentrosomal microtubules originating from the trans-Golgi network (TGN). Required for the polarization of the cytoplasmic microtubule arrays in migrating cells towards the leading edge of the cell. May act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus-ends to cortical platforms composed of ERC1 and PHLDB2. This cortical microtubule stabilizing activity is regulated at least in part by phosphatidylinositol 3-kinase signaling. Also performs a similar stabilizing function at the kinetochore which is essential for the bipolar alignment of chromosomes on the mitotic spindle. Acts as a mediator of ERBB2-dependent stabilization of microtubules at the cell cortex (By similarity). 
Sequence Annotation
 REPEAT 179 214 HEAT 1.
 REPEAT 215 251 HEAT 2.
 REPEAT 764 801 HEAT 3.
 REPEAT 1090 1127 HEAT 4.
 REPEAT 1208 1245 HEAT 5.
 REGION 1 40 Golgi localization (By similarity).
 REGION 450 565 Interaction with microtubules, MAPRE1 and
 REGION 864 1286 Interaction with RSN and localization to
 REGION 1009 1286 Required for cortical localization (By
 MOTIF 500 503 Microtubule tip localization signal.
 MOTIF 523 526 Microtubule tip localization signal.
 MOD_RES 14 14 Phosphoserine (By similarity).
 MOD_RES 376 376 Phosphoserine (By similarity).
 MOD_RES 461 461 Phosphoserine (By similarity).
 MOD_RES 513 513 Phosphoserine (By similarity).
 MOD_RES 531 531 Phosphoserine (By similarity).
 MOD_RES 535 535 Phosphoserine (By similarity).
 MOD_RES 581 581 Phosphoserine (By similarity).
 MOD_RES 1021 1021 Phosphoserine (By similarity).  
Keyword
 Cell cycle; Cell division; Cell membrane; Cell projection; Centromere; Chromosome; Coiled coil; Complete proteome; Cytoplasm; Cytoskeleton; Golgi apparatus; Kinetochore; Membrane; Microtubule; Mitosis; Phosphoprotein; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1286 AA 
Protein Sequence
MRRLICKRIC DYKSFDDEES VDGNRPSSAA SAFKVPAPKT PGNPVNSARK PGSAGGPKAG 60
GTSKEGGAGA VDEDDFIKAF TDVPSIQIYS SRELEETLNK IREILSDDKH DWDQRANALK 120
KIRSLLVAGA AQYDCFFQHL RLLDGALKLS AKDLRSQVVR EACITVAHLS TVLGNKFDHG 180
AEAIVPTLFN LVPNSAKVMA TSGCAAIRFI IRHTHVPRLI PLITSNCTSK SVPVRRRSFE 240
FLDLLLQEWQ THSLERHAAV LVETIKKGIH DADAEARVEA RKTYMGLRNH FPGEAETLYN 300
SLEPSYQKSL QTYLKSSGSV ASLPQSDRSS SSSQESLNRP FSSKWSTANP SAVAGRVSVG 360
GSKASPLPGS LQRSRSDIDV NAAAGAKAHH AAGQAVRSGR LGAGALNPGS YASLEDTSDK 420
MDGTASEDGR VRAKLSTPLV AVGNAKTDSR GRSRTKMVSQ SQPGSRSGSP GRVLTTTALS 480
TVSSGAQRIL VNSASAQKRS KIPRSQGCSR EASPSRLSVA RSSRIPRPSV SQGCSREASR 540
ESSRDTSPVR SFQPLGPGYG MSQSSRLSSS VSAMRVLNTG SDVEEAVADA LLLGDIRTKK 600
KPARRRYESY GMHSDDDANS DASSACSERS YSSRNGSIPT YMRQTEDVAE VLNRCASSNW 660
SERKEGLLGL QNLLKNQRTL SRVELKRLCE IFTRMFADPH GKVFSMFLET LVDFIQVHKD 720
DLQDWLFVLL TQLLKKMGAD LLGSVQAKVQ KALDVTRESF PNDLQFNILM RFTVDQTQTP 780
SLKVKVAILK YIETLAKQMD PGDFINSSET RLAVSRVITW TTEPKSSDVR KAAQSVLISL 840
FELNTPEFTM LLGALPKTFQ DGATKLLHNH LRNTGNGTQS SMGSPLTRPT PRSPANWSSP 900
LTSPTNTSQN TLSPSAFDYD TENMNSEDIY SSLRGVTEAI QNFSFRSQED MSEPLKRDPK 960
KEDGDTVCSG PGMSDPRAGG DAPDSSQPAL DNKASLLHSV PLHSSPRSRD YNPYNYSDSI 1020
SPFNKSALKE AMFDDDADQF PDDLSLDHSD LVAELLKELS NHNERIEERK IALYELMKLT 1080
QEESFSVWDE HFKTILLLLL ETLGDKEPTI RALALKVLKE ILRHQPARFK NYAELTVMKT 1140
LEAHKDPHKE VVRSAEEAAS VLATSISPEQ CIKVLCPIIQ TADYPINLAA IKMQTKVIER 1200
VSKETLNLLL PEIMPGLIQG YDNSESSVRK ACVFCLVAVH AVIGDELKPH LSQLTGSKMK 1260
LLNLYIKRAQ TGSAGADPTT DVSGQS 1286 
Gene Ontology
 GO:0000777; C:condensed chromosome kinetochore; IEA:UniProtKB-SubCell.
 GO:0005881; C:cytoplasmic microtubule; IEA:Compara.
 GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.
 GO:0005874; C:microtubule; IDA:RGD.
 GO:0005815; C:microtubule organizing center; IEA:UniProtKB-SubCell.
 GO:0032587; C:ruffle membrane; ISS:UniProtKB.
 GO:0005819; C:spindle; IEA:UniProtKB-SubCell.
 GO:0005802; C:trans-Golgi network; ISS:UniProtKB.
 GO:0051010; F:microtubule plus-end binding; ISS:UniProtKB.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0016477; P:cell migration; IEA:Compara.
 GO:0007163; P:establishment or maintenance of cell polarity; IEA:Compara.
 GO:0034453; P:microtubule anchoring; ISS:UniProtKB.
 GO:0007020; P:microtubule nucleation; ISS:UniProtKB.
 GO:0031023; P:microtubule organizing center organization; ISS:UniProtKB.
 GO:0007067; P:mitosis; IEA:UniProtKB-KW.
 GO:0007026; P:negative regulation of microtubule depolymerization; IDA:RGD. 
Interpro
 IPR011989; ARM-like.
 IPR016024; ARM-type_fold.
 IPR024395; CLASP_N_dom. 
Pfam
 PF12348; CLASP_N 
SMART
  
PROSITE
 PS50077; HEAT_REPEAT 
PRINTS