CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006194
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Pre-mRNA-splicing helicase BRR2 
Protein Synonyms/Alias
 Protein Snu246 
Gene Name
 BRR2 
Gene Synonyms/Alias
 RSS1; SNU246; YER172C; SYGP-ORF66 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
304LRSELGYKDTSVIQDacetylation[1]
930EVVAGNIKCRNDAVNubiquitination[2]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 RNA helicase that plays an essential role in pre-mRNA splicing as component of the U5 snRNP and U4/U6-U5 tri-snRNP complexes. Involved in spliceosome assembly, activation and disassembly. Mediates changes in the dynamic network of RNA-RNA interactions in the spliceosome. Catalyzes the ATP-dependent unwinding of U4/U6 RNA duplices, an essential step in the assembly of a catalytically active spliceosome. 
Sequence Annotation
 DOMAIN 508 692 Helicase ATP-binding 1.
 DOMAIN 718 921 Helicase C-terminal.
 DOMAIN 998 1308 SEC63 1.
 DOMAIN 1357 1533 Helicase ATP-binding 2.
 DOMAIN 1846 2160 SEC63 2.
 NP_BIND 521 528 ATP (Potential).
 NP_BIND 1370 1377 ATP (Potential).
 MOTIF 634 637 DEIH box.
 MOTIF 1474 1477 DDAH box.
 MOD_RES 403 403 Phosphoserine.  
Keyword
 3D-structure; ATP-binding; Complete proteome; Helicase; Hydrolase; mRNA processing; mRNA splicing; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Repeat; Spliceosome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 2163 AA 
Protein Sequence
MTEHETKDKA KKIREIYRYD EMSNKVLKVD KRFMNTSQNP QRDAEISQPK SMSGRISAKD 60
MGQGLCNNIN KGLKENDVAV EKTGKSASLK KIQQHNTILN SSSDFRLHYY PKDPSNVETY 120
EQILQWVTEV LGNDIPHDLI IGTADIFIRQ LKENEENEDG NIEERKEKIQ HELGINIDSL 180
KFNELVKLMK NITDYETHPD NSNKQAVAIL ADDEKSDEEE VTEMSNNANV LGGEINDNED 240
DDEEYDYNDV EVNSKKKNKR ALPNIENDII KLSDSKTSNI ESVPIYSIDE FFLQRKLRSE 300
LGYKDTSVIQ DLSEKILNDI ETLEHNPVAL EQKLVDLLKF ENISLAEFIL KNRSTIFWGI 360
RLAKSTENEI PNLIEKMVAK GLNDLVEQYK FRETTHSKRE LDSGDDQPQS SEAKRTKFSN 420
PAIPPVIDLE KIKFDESSKL MTVTKVSLPE GSFKRVKPQY DEIHIPAPSK PVIDYELKEI 480
TSLPDWCQEA FPSSETTSLN PIQSKVFHAA FEGDSNMLIC APTGSGKTNI ALLTVLKALS 540
HHYNPKTKKL NLSAFKIVYI APLKALVQEQ VREFQRRLAF LGIKVAELTG DSRLSRKQID 600
ETQVLVSTPE KWDITTRNSN NLAIVELVRL LIIDEIHLLH DDRGPVLESI VARTFWASKY 660
GQEYPRIIGL SATLPNYEDV GRFLRVPKEG LFYFDSSFRP CPLSQQFCGI KERNSLKKLK 720
AMNDACYEKV LESINEGNQI IVFVHSRKET SRTATWLKNK FAEENITHKL TKNDAGSKQI 780
LKTEAANVLD PSLRKLIESG IGTHHAGLTR SDRSLSEDLF ADGLLQVLVC TATLAWGVNL 840
PAHTVIIKGT DVYSPEKGSW EQLSPQDVLQ MLGRAGRPRY DTFGEGIIIT DQSNVQYYLS 900
VLNQQLPIES QFVSKLVDNL NAEVVAGNIK CRNDAVNWLA YTYLYVRMLA SPMLYKVPDI 960
SSDGQLKKFR ESLVHSALCI LKEQELVLYD AENDVIEATD LGNIASSFYI NHASMDVYNR 1020
ELDEHTTQID LFRIFSMSEE FKYVSVRYEE KRELKQLLEK APIPIREDID DPLAKVNVLL 1080
QSYFSQLKFE GFALNSDIVF IHQNAGRLLR AMFEICLKRG WGHPTRMLLN LCKSATTKMW 1140
PTNCPLRQFK TCPVEVIKRL EASTVPWGDY LQLETPAEVG RAIRSEKYGK QVYDLLKRFP 1200
KMSVTCNAQP ITRSVMRFNI EIIADWIWDM NVHGSLEPFL LMLEDTDGDS ILYYDVLFIT 1260
PDIVGHEFTL SFTYELKQHN QNNLPPNFFL TLISENWWHS EFEIPVSFNG FKLPKKFPPP 1320
TPLLENISIS TSELGNDDFS EVFEFKTFNK IQSQVFESLY NSNDSVFVGS GKGTGKTAMA 1380
ELALLNHWRQ NKGRAVYINP SGEKIDFLLS DWNKRFSHLA GGKIINKLGN DPSLNLKLLA 1440
KSHVLLATPV QFELLSRRWR QRKNIQSLEL MIYDDAHEIS QGVYGAVYET LISRMIFIAT 1500
QLEKKIRFVC LSNCLANARD FGEWAGMTKS NIYNFSPSER IEPLEINIQS FKDVEHISFN 1560
FSMLQMAFEA SAAAAGNRNS SSVFLPSRKD CMEVASAFMK FSKAIEWDML NVEEEQIVPY 1620
IEKLTDGHLR APLKHGVGIL YKGMASNDER IVKRLYEYGA VSVLLISKDC SAFACKTDEV 1680
IILGTNLYDG AEHKYMPYTI NELLEMVGLA SGNDSMAGKV LILTSHNMKA YYKKFLIEPL 1740
PTESYLQYII HDTLNNEIAN SIIQSKQDCV DWFTYSYFYR RIHVNPSYYG VRDTSPHGIS 1800
VFLSNLVETC LNDLVESSFI EIDDTEAEVT AEVNGGDDEA TEIISTLSNG LIASHYGVSF 1860
FTIQSFVSSL SNTSTLKNML YVLSTAVEFE SVPLRKGDRA LLVKLSKRLP LRFPEHTSSG 1920
SVSFKVFLLL QAYFSRLELP VDFQNDLKDI LEKVVPLINV VVDILSANGY LNATTAMDLA 1980
QMLIQGVWDV DNPLRQIPHF NNKILEKCKE INVETVYDIM ALEDEERDEI LTLTDSQLAQ 2040
VAAFVNNYPN VELTYSLNNS DSLISGVKQK ITIQLTRDVE PENLQVTSEK YPFDKLESWW 2100
LVLGEVSKKE LYAIKKVTLN KETQQYELEF DTPTSGKHNL TIWCVCDSYL DADKELSFEI 2160
NVK 2163 
Gene Ontology
 GO:0005681; C:spliceosomal complex; IEA:UniProtKB-KW.
 GO:0046540; C:U4/U6 x U5 tri-snRNP complex; IDA:SGD.
 GO:0005682; C:U5 snRNP; IDA:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004004; F:ATP-dependent RNA helicase activity; IMP:SGD.
 GO:0003676; F:nucleic acid binding; IEA:InterPro.
 GO:0000388; P:spliceosome conformational change to release U4 (or U4atac) and U1 (or U11); IMP:SGD. 
Interpro
 IPR011545; DNA/RNA_helicase_DEAD/DEAH_N.
 IPR014001; Helicase_ATP-bd.
 IPR001650; Helicase_C.
 IPR027417; P-loop_NTPase.
 IPR004179; Sec63-dom. 
Pfam
 PF00270; DEAD
 PF00271; Helicase_C
 PF02889; Sec63 
SMART
 SM00487; DEXDc
 SM00490; HELICc
 SM00611; SEC63
 SM00973; Sec63 
PROSITE
 PS51192; HELICASE_ATP_BIND_1
 PS51194; HELICASE_CTER 
PRINTS