CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012307
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Protein FAM50A 
Protein Synonyms/Alias
 Protein HXC-26; Protein XAP-5 
Gene Name
 FAM50A 
Gene Synonyms/Alias
 DXS9928E; HXC26; XAP5 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
5***MAQYKGAASEAGmethylation[1]
5***MAQYKGAASEAGubiquitination[2, 3, 4]
271FYDFIVTKARGKSGPubiquitination[5, 6]
275IVTKARGKSGPLFNFubiquitination[3]
305KDESHAGKVVLRSWYubiquitination[3, 4]
Reference
 [1] A general molecular affinity strategy for global detection and proteomic analysis of lysine methylation.
 Moore KE, Carlson SM, Camp ND, Cheung P, James RG, Chua KF, Wolf-Yadlin A, Gozani O.
 Mol Cell. 2013 May 9;50(3):444-56. [PMID: 23583077]
 [2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [6] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789
Functional Description
 May be a DNA-binding protein or transcriptional factor. 
Sequence Annotation
 MOTIF 152 155 Nuclear localization signal (Potential).  
Keyword
 Complete proteome; Direct protein sequencing; Nucleus; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 339 AA 
Protein Sequence
MAQYKGAASE AGRAMHLMKK REKQREQMEQ MKQRIAEENI MKSNIDKKFS AHYDAVEAEL 60
KSSTVGLVTL NDMKAKQEAL VKEREKQLAK KEQSKELQMK LEKLREKERK KEAKRKISSL 120
SFTLEEEEEG GEEEEEAAMY EEEMEREEIT TKKRKLGKNP DVDTSFLPDR DREEEENRLR 180
EELRQEWEAK QEKIKSEEIE ITFSYWDGSG HRRTVKMRKG NTMQQFLQKA LEILRKDFSE 240
LRSAGVEQLM YIKEDLIIPH HHSFYDFIVT KARGKSGPLF NFDVHDDVRL LSDATVEKDE 300
SHAGKVVLRS WYEKNKHIFP ASRWEPYDPE KKWDKYTIR 339 
Gene Ontology
 GO:0005634; C:nucleus; NAS:UniProtKB.
 GO:0007283; P:spermatogenesis; NAS:UniProtKB. 
Interpro
 IPR007005; XAP5. 
Pfam
 PF04921; XAP5 
SMART
  
PROSITE
  
PRINTS