CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-028628
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 E3 ubiquitin-protein ligase HUWE1 
Protein Synonyms/Alias
  
Gene Name
 Huwe1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
1107STASALTKLLTKGLSubiquitination[1]
1470DLIMTAIKRNGADYRubiquitination[1]
1733LESTNTEKEASLDETubiquitination[1]
1917EFENLRIKGPNAVQLubiquitination[1]
1931LVKTTPLKPSSLPVIacetylation[2]
2249NTVNAALKPLETLSRacetylation[2]
2268PSSLFGSKSASSKNKacetylation[2, 3]
2268PSSLFGSKSASSKNKubiquitination[1]
2672HDCVSVVKVPIVNHLubiquitination[1]
2704QLAEEETKIIDKGKEubiquitination[1]
3245ELCIETPKLSTSEERubiquitination[1]
3358HFTQQRTKETNCESDubiquitination[1]
3454RSSLLTEKLLRLLSLubiquitination[1]
3916ESQLAHIKDEPPPLSubiquitination[1]
4138YFTRSFYKHILGKSVubiquitination[1]
4299FDQADRAKFLQFVTGubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [3] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337] 
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Ligase; Reference proteome; Ubl conjugation pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 4378 AA 
Protein Sequence
MKVDRTKLKK TPTEAPADCR ALIDKLKVCN DEQLLLELQQ IKTWNIGKCE LYHWVDLLDR 60
FDGILADAGQ TVENMSWMLV CDRPEKEQLK MLLLAVLNFT ALLIEYSFSR HLYSSIEHLT 120
TLLASSDMQV VLAVLNLLYV FSKRSNYITR LGSDKRTPLL TRLQHLAESW GGKENGFGLA 180
ECCRDLQMLK YPPSATTLHF EFYADPGAEV KIEKRTTSNT LHYIHIEQLD KISESPSEIM 240
ESLTKMYSIP KDKQMLLFTH IRLAHGFSNH RKRLQAVQAR LHAISILVYS NALQESANSI 300
LYNGLIEELV DVLQITDKQL MEIKAASLRT LTSIVHLERT PKLSSIIDCT GTASYHGFLP 360
VLVRNCIQAM IDPSMDPYPH QFATALFSFL YHLASYDAGG EALVSCGMME ALLKVIKFLG 420
DEQDQITFVT RAVRVVDLIT NLDMAAFQSH SGLSIFIYRL EHEVDLCRKE CPFVIKPKIQ 480
RPSTTQEGEE METDMDGVQC IPQRAALLKS MLNFLKKAIQ DPAFSDGIRH VMDGSLPTSL 540
KHIISNAEYY GPSLFLLATE VVTVFVFQEP SLLSSLQDNG LTDVMLHALL IKDVPATREV 600
LGSLPNVFSA LCLNARGLQS FVQCQPFERL FKVLLSPDYL PAMRRRRSSD PLGDTASNLG 660
SAVDELMRHQ PTLKTDATTA IIKLLEEICN LGRDPKYICQ KPSIQKADGT ATAPPPRSNH 720
AAEEASSEDE EEEEVQAMQS FNSAQQNETE PNQQVVGTEE RIPIPLMDYI LNVMKFVESI 780
LSNNTTDDHC QEFVNQKGLL PLVTILGLPN LPIDFPTSAA CQAVAGVCKS ILTLSHEPKV 840
LQEGLLQLDL ILSSLEPLHR PIESPGGSVL LRELACAGNV ADATLSAQAT PLLHALTAAH 900
AYIMMFVHTC RVGQSEIRSI SVNQWGSQLG LSVLSKLSQL YCSLVWESTV LLSLCTPNSL 960
PSGCEFGQAD MQKLVPKDEK AGTTQGGKRS DGEQDGTAGS MDASAQGLLE GIELDGDTLA 1020
PMETDEPSSS DSKGKSKITP AMAARIKQIK PLLSASSRLG RALAELFGLL VKLCVGSPVR 1080
QRRSHHAAST TTAPTPAARS TASALTKLLT KGLSWQPPPY TPTPRFRLTF FICSVGFTSP 1140
MLFDERKYPY HLMLQKFLCS GGHNALFETF NWALSMGGKV PVSEGLEHSD LPDGTGEFLD 1200
AWLMLVEKMV NPTTVLESPH SLPAKLPGGV QSFPQFSALR FLVVTQKAAF TCIKNLWNRK 1260
PLKVYGGRMA ESMLAILCHI LRGEPVIRER LSKEKEGSRG EEEAGQEEGG SRREPQVNQQ 1320
QLQQLMDMGF TREHAMEALL NTSTMEQATE YLLTHPPPII GGVVRDLSMS EEDQMMRAIA 1380
MSLGQDIPMD QRAESPEEVA CRKEEEERKA REKQEEEEAK CLEKFQDADP LEQDELHTFT 1440
DTMLPGCFHL LDELPDTVYR VCDLIMTAIK RNGADYRDMI LKQVVNQVWE AADVLIKAAL 1500
PLTTSDTKTV SEWISQMATL PQASNLATRI LLLTLLFEEL KLPCAWVVES SGILNVLIKL 1560
LEVVQPCLQA AKEQKEVQTP KWITPVLLLI DFYEKTAISS KRRAQMTKYL QSNSNNWRWF 1620
DDRSGRWCSY SASNNSTIDS AWKSGETSVR FTAGRRRYTV QFTTMVQVNE ETGNRRPVML 1680
TLLRVPRLSK NSKSSNGQEL EKTLEESKET DIKHKENKGN DIPLALESTN TEKEASLDET 1740
KIGEILIQGL TEDMVTVLIR ACVSMLGVPV DPDTLHATLR LCLRLTRDHK YAMMFAELKS 1800
TRMILNLTQS SGFNGFTPLV TLLLRHIIED PCTLRHTMEK VVRSAATSGA GSTTSGVVSG 1860
SLGSREINYI LRVLGPAACR NPDIFTEVAN CCIRIALPAP RGSGTASDDE FENLRIKGPN 1920
AVQLVKTTPL KPSSLPVIPD TIKEVIYDML NALAAYHAPE EADKSDPKPG GTTQEVGQLL 1980
QDMGDDVYQQ YRSLTRQSSD FDTQSGFSLN SQVFAADGAP AETSTTGTSQ GEGASTPEET 2040
REGKKDKEGD RTSEEGKQKS KGSKPLMPTS TILRLLAELV RSYVGIATLI ANYSYTVGQS 2100
ELIKEDCSVL AFVLDHLLPH TQNAEDKDTP ALARLFLASL AAAGSGTDAQ VALVNEVKAA 2160
LGRALAMAES TEKHARLQAV MCIISTIMES CPSTSSFYSS ATAKTQHNGM NNIIRLFLKK 2220
GLVNDLARVP HSLDLSSPNM ANTVNAALKP LETLSRIVNQ PSSLFGSKSA SSKNKSEQDA 2280
QGASQDSSSH QQDPGEPGEA EVQEEDHDVT QTEVADGDIM DGEAETDSVV IAGQPEVLSS 2340
QEMQVENELE DLIDELLERD GGSGNSTIIV SRSGEDESQE DVLMDEAPSN LSQASTLQAN 2400
REDSMNILDP EDEEEHTQEE DSSGSNEDED DSQDEEEEEE EDEEDDQEDD EGEEGDEDDD 2460
DDGSEMELDE DYPDMNASPL VRFERFDRED DLIIEFDNMF SSATDIPPSP GNIPTTHPLM 2520
VRHADHSSLT LGSGSSTTRL TQGIGRSQRT LRQLTANTGH TIHVHYPGNR QPNPPLILQR 2580
LLGPSAAADI LQLSSSLPLQ SRGRARLLVG NDDVHIIARS DDELLDDFFH DQSTATSQAG 2640
TLSSIPTALT RWTEECKVLD AESMHDCVSV VKVPIVNHLE FLRDEELEER REKRRKQLAE 2700
EETKIIDKGK EDKENRDQSA QCTVTKTNDS TEQNVSDGTP MPDSYPTTPS STDAPTSESK 2760
ETLGTLQPSQ QQPALPPPPS LGEIPQELQS PAEEVANSTQ LLMPIELEEL GPTRPSGEAE 2820
TTQMELSPAP TITSLSPERA EDSDALTAVS SQLEGSPMDT SSLASCTLEE AVGDTPAAGS 2880
SEQPTAGSST PGDAPSVVAE VQGRPDVSRE SNQPPEDSSP PASSESSSTR DSAVAISGAD 2940
SRGILEEPLP STSSEEEDPL AGISLPEGVD PSFLAALPDD IRREVLQNQL GIRPPTRSAP 3000
SSNSSAPAVV GNPGVTEVSP EFLAALPPAI QEEVLAQQRA EQQRRELAQN ASSDTPMDPV 3060
TFIQTLPSDL RRSVLEDMED SVLAVMPPDI AAEAQALRRE QEARQRQLMH ERLFGHSSTS 3120
ALSAILRSPA FTSRLSGNRG VQYTRLAVQR GGTFQMGGSS SHNRPSGSNV DTLLRLRGRL 3180
LLDHEALSCL LVLLFVDEPK LNTSRLHRVL RNLCYHAQTR HWVIRSLLSI LQRSSESELC 3240
IETPKLSTSE ERGKKSSKSC ASSSHENRPL DLLHKMESKS SNQLSWLSVS MDAALGCRTN 3300
IFQIQRSGGR KHTEKHASSG STVHIHPQAA PVVCRHVLDT LIQLAKVFPS HFTQQRTKET 3360
NCESDRERGS KQACSPCSSQ SSSSGICTDF WDLLVKLDNM NVSRKGKNSV KSVPVSSGGE 3420
GETSPHSLEA SPLGQLMNML SHPVIRRSSL LTEKLLRLLS LISIALPENK VSEVQTNSSN 3480
SGSSTAATSN TSTTTTTTTT ATAPTPTPPA ATTPVTSAPA LVAATAISTI TVAASTTVTT 3540
PTTATTTVST STTKGSKSPA KVGEGGSGID FKMVSSGLTE NQLQLSVEVL TSHSCSEEGL 3600
EDAANVLLQL SRGDSGTRDT VLKLLLNGAR HLGYTLCKQI GTLLAELREY NLEQQRRAQC 3660
ETLSPDGLPE EQPQTTKLKG KMQSRFDMAE NVVIVASQKR PLGGRELQLP SMSMLTSKTS 3720
TQKFFLRVLQ VIIQLRDDTR RANKKAKQTG RLGSSGLGSA SSIQAAVRQL EAEADAIIQM 3780
VREGQRARRQ QQAATSESSN QSETSVRREE SPMDVDQPSP SAQDTQSIVI SDGTPQGEKE 3840
KEEKPPELPL LSEQLSLDEL WDMLGECLKE LEESHDQHAV LVLQPAVEAF FLVHATERES 3900
KPPVRDTRES QLAHIKDEPP PLSPAPLTPA TPSSLDPFFS REPSSMHISS SLPPDTQKFL 3960
RFAETHRTVL NQILRQSTTH LADGPFAVLV DYIRVLDFDV KRKYFRQELE RLDEGLRKED 4020
MAVHVRRDHV FEDSYRELHR KSPEEMKNRL YIVFEGEEGQ DAGGLLREWY MIISREMFNP 4080
MYALFRTSPG DRVTYTINPS SHCNPNHLSY FKFVGRIVAK AVYDNRLLEC YFTRSFYKHI 4140
LGKSVRYTDM ESEDYHFYQG LVYLLENDVS TLGYDLTFST EVQEFGVCEV RDLKPNGANI 4200
LVTEENKKEY VHLVCQMRMT GAIRKQLAAF LEGFYEIIPK RLISIFTEQE LELLISGLPT 4260
IDIDDLKSNT EYHKYQSNSI QIQWFWRALR SFDQADRAKF LQFVTGTSKV PLQGFAALEG 4320
MNGIQKFQIH RDDRSTDRLP SAHTCFNQLD LPAYESFEKL RHMLLLAIQE CSEGFGLA 4378 
Gene Ontology
 GO:0005737; C:cytoplasm; IBA:RefGenome.
 GO:0005634; C:nucleus; IBA:RefGenome.
 GO:0004842; F:ubiquitin-protein ligase activity; IBA:RefGenome.
 GO:0006284; P:base-excision repair; IEA:Compara.
 GO:0016574; P:histone ubiquitination; IEA:Compara.
 GO:0006513; P:protein monoubiquitination; IEA:Compara.
 GO:0000209; P:protein polyubiquitination; IEA:Compara.
 GO:0042787; P:protein ubiquitination involved in ubiquitin-dependent protein catabolic process; IBA:RefGenome. 
Interpro
 IPR016024; ARM-type_fold.
 IPR025527; DUF4414.
 IPR010309; E3_Ub_ligase_DUF908.
 IPR010314; E3_Ub_ligase_DUF913.
 IPR000569; HECT.
 IPR009060; UBA-like.
 IPR000449; UBA/transl_elong_EF1B_N.
 IPR015940; UBA/transl_elong_EF1B_N_euk.
 IPR004170; WWE-dom. 
Pfam
 PF14377; DUF4414
 PF06012; DUF908
 PF06025; DUF913
 PF00632; HECT
 PF00627; UBA
 PF02825; WWE 
SMART
 SM00119; HECTc
 SM00165; UBA 
PROSITE
 PS50237; HECT
 PS50030; UBA
 PS50918; WWE 
PRINTS