UniProt Accession

 ALKB3_HUMAN; Q96Q83; A6NDJ1; Q3SYI0; Q6NX57; Q96BU8 

Genbank Protein ID

 AB042029; DQ196343; AC087521; CH471064; BC015155; BC103812; BC103813; BC103814; BC067257 

Genbank Nucleotide ID

 BAB70508.1; ABA27096.1; EAW68078.1; AAH15155.1; AAI03813.1; AAI03814.1; AAI03815.1; AAH67257.1 

Protein Name

 Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3 

Protein Synonyms/Alias

 Alkylated DNA repair protein alkB homolog 3; DEPC-1; Prostate cancer antigen 1 

Gene Name

 ALKBH3 

Gene Synonyms/Alias

 ABH3; DEPC1 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
19	GAWAAPVKSQAIAQP	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 Dioxygenase that repairs alkylated DNA containing 1- methyladenine (1meA) and 3-methylcytosine (3meC) by oxidative demethylation. Has a strong preference for single-stranded DNA. Able to process alkylated 3mC within double-stranded regions via its interaction with ASCC3, which promotes DNA unwinding to generate single-stranded substrate needed for ALKHB3. May also act on RNA. Requires molecular oxygen, alpha-ketoglutarate and iron. 

Sequence Annotation

 DOMAIN 172 278 Fe2OG dioxygenase.
 REGION 141 143 Substrate binding (By similarity).
 REGION 179 181 Alpha-ketoglutarate binding.
 REGION 269 275 Alpha-ketoglutarate binding.
 METAL 191 191 Iron; catalytic.
 METAL 193 193 Iron; catalytic.
 METAL 257 257 Iron; catalytic.
 BINDING 115 115 Substrate (By similarity).
 BINDING 194 194 Substrate (By similarity).  

Keyword

 3D-structure; Alternative splicing; Complete proteome; Cytoplasm; Dioxygenase; DNA damage; DNA repair; Iron; Metal-binding; Nucleus; Oxidoreductase; Polymorphism; Reference proteome; Vitamin C. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 286 AA 

Protein Sequence

Gene Ontology

 GO:0005739; C:mitochondrion; IDA:HPA.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0051747; F:cytosine C-5 DNA demethylase activity; IEA:Compara.
 GO:0043734; F:DNA-N1-methyladenine dioxygenase activity; IDA:UniProtKB.
 GO:0008198; F:ferrous iron binding; IDA:UniProtKB.
 GO:0031418; F:L-ascorbic acid binding; IEA:UniProtKB-KW.
 GO:0016702; F:oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen; IEA:UniProtKB-KW.
 GO:0008283; P:cell proliferation; IMP:UniProtKB.
 GO:0006307; P:DNA dealkylation involved in DNA repair; IDA:UniProtKB.
 GO:0035552; P:oxidative single-stranded DNA demethylation; IDA:UniProtKB. 

Interpro

 IPR027450; AlkB-like.
 IPR005123; Oxoglu/Fe-dep_dioxygenase. 

Pfam

 PF13532; 2OG-FeII_Oxy_2 

SMART

  

PROSITE

 PS51471; FE2OG_OXY 

PRINTS