UniProt Accession

 RIR4_YEAST; P49723; D6VUW4; Q66R92 

Genbank Protein ID

 U30385; Z72965; AY723819; BK006941 

Genbank Nucleotide ID

 AAB72236.1; CAA97206.1; AAU09736.1; DAA08275.1 

Protein Name

 Ribonucleoside-diphosphate reductase small chain 2 

Protein Synonyms/Alias

 Ribonucleotide reductase R2 subunit 2; Ribonucleotide reductase small subunit 2 

Gene Name

 RNR4 

Gene Synonyms/Alias

 CRT3; YGR180C 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
9	EAHNQFLKTFQKERH	acetylation	[1]
23	HDMKEAEKDEILLME	acetylation	[1]
50	HEIWAAYKKVEASFW	acetylation	[1]
50	HEIWAAYKKVEASFW	ubiquitination	[2, 3]
73	KDTEDFQKLTDDQKT	acetylation	[1]
143	DAFFKDPKNIPLFKE	acetylation	[1]
149	PKNIPLFKEIANLPE	acetylation	[1]
158	IANLPEVKHKAAFIE	acetylation	[1]
160	NLPEVKHKAAFIERW	ubiquitination	[2]
266	SNSLPVEKFGMDLKS	acetylation	[1]
318	GKTTFFEKKVSDYQK	ubiquitination	[3]
325	KKVSDYQKASDMSKS	acetylation	[1]
325	KKVSDYQKASDMSKS	ubiquitination	[3]
331	QKASDMSKSATPSKE	ubiquitination	[3]
337	SKSATPSKEINFDDD	ubiquitination	[2, 3]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047]
 [3] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 

Functional Description

 Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. RNR4 is required for proper folding of RNR2 and assembly with the large subunits. 

Sequence Annotation

 ACT_SITE 131 131 By similarity.
 MOD_RES 1 1 N-acetylmethionine.
 MOD_RES 169 169 Phosphoserine.
 MOD_RES 332 332 Phosphoserine.
 MOD_RES 334 334 Phosphothreonine.
 MOD_RES 336 336 Phosphoserine.  

Keyword

 3D-structure; Acetylation; Complete proteome; Direct protein sequencing; DNA replication; Nucleus; Oxidoreductase; Phosphoprotein; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 345 AA 

Protein Sequence

Gene Ontology

 GO:0005634; C:nucleus; IDA:SGD.
 GO:0005971; C:ribonucleoside-diphosphate reductase complex; IDA:SGD.
 GO:0004748; F:ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor; IMP:SGD.
 GO:0046914; F:transition metal ion binding; IEA:InterPro.
 GO:0051188; P:cofactor biosynthetic process; IMP:SGD.
 GO:0009186; P:deoxyribonucleoside diphosphate metabolic process; IEA:InterPro.
 GO:0009263; P:deoxyribonucleotide biosynthetic process; IDA:SGD.
 GO:0006260; P:DNA replication; IEA:UniProtKB-UniPathway. 

Interpro

 IPR009078; Ferritin-like_SF.
 IPR012348; RNR-rel.
 IPR000358; RNR_small. 

Pfam

 PF00268; Ribonuc_red_sm 

SMART

  

PROSITE

 PS00368; RIBORED_SMALL 

PRINTS