UniProt Accession

 NB5R3_HUMAN; P00387; B1AHF2; B7Z7L3; O75675; Q8TDL8; Q8WTS8; Q9UEN4; Q9UEN5; Q9UL55; Q9UL56 

Genbank Protein ID

 M28713; M28705; M28706; M28707; M28708; M28709; M28710; M28711; Y09501; AF361370; AJ010116; AJ010117; AJ010118; AY341030; BT009821; CR456435; AF061830; AF061831; AK302204; Z93241; Z93241; BC004821; AJ310899; AJ310900; M16461; M16462 

Genbank Nucleotide ID

 AAA59900.1; AAA59900.1; AAA59900.1; AAA59900.1; AAA59900.1; AAA59900.1; AAA59900.1; AAA59900.1; CAA70696.1; AAL87744.1; CAA09006.1; CAA09007.1; CAA09008.1; AAP88936.1; AAP88823.1; CAG30321.1; AAF06818.1; AAF06819.1; BAH13649.1; CAB42843.1; CAQ08414.1; AAH04821.1; CAC84523.1; CAC84524.1; AAA52306.1; AAA52307.1 

Protein Name

 NADH-cytochrome b5 reductase 3 

Protein Synonyms/Alias

 B5R; Cytochrome b5 reductase; Diaphorase-1; NADH-cytochrome b5 reductase 3 membrane-bound form; NADH-cytochrome b5 reductase 3 soluble form 

Gene Name

 CYB5R3 

Gene Synonyms/Alias

 DIA1 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
75	TLESPDIKYPLRLID	ubiquitination	[1]
153	YFKDTHPKFPAGGKM	ubiquitination	[1]
159	PKFPAGGKMSQYLES	ubiquitination	[1]
189	LVYQGKGKFAIRPDK	ubiquitination	[2]
196	KFAIRPDKKSNPIIR	ubiquitination	[1, 2]
206	NPIIRTVKSVGMIAG	ubiquitination	[1, 2]
247	LFANQTEKDILLRPE	ubiquitination	[1]
267	NKHSARFKLWYTLDR	ubiquitination	[1, 2]

Reference

 [1] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 Desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction. 

Sequence Annotation

 DOMAIN 40 152 FAD-binding FR-type.
 NP_BIND 132 147 FAD (By similarity).
 NP_BIND 171 206 FAD (By similarity).
 MOD_RES 42 42 N6-acetyllysine.
 MOD_RES 43 43 Phosphotyrosine.
 MOD_RES 120 120 N6-acetyllysine (By similarity).
 MOD_RES 130 130 Phosphotyrosine (By similarity).
 LIPID 2 2 N-myristoyl glycine.  

Keyword

 3D-structure; Acetylation; Alternative promoter usage; Alternative splicing; Cholesterol biosynthesis; Cholesterol metabolism; Complete proteome; Cytoplasm; Direct protein sequencing; Disease mutation; Endoplasmic reticulum; FAD; Flavoprotein; Lipid biosynthesis; Lipid metabolism; Lipoprotein; Membrane; Mitochondrion; Mitochondrion outer membrane; Myristate; NAD; Oxidoreductase; Phosphoprotein; Polymorphism; Reference proteome; Steroid biosynthesis; Steroid metabolism; Sterol biosynthesis; Sterol metabolism. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 301 AA 

Protein Sequence

Gene Ontology

 GO:0005783; C:endoplasmic reticulum; IDA:HPA.
 GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
 GO:0005833; C:hemoglobin complex; TAS:ProtInc.
 GO:0005811; C:lipid particle; IDA:UniProtKB.
 GO:0005743; C:mitochondrial inner membrane; IEA:Compara.
 GO:0005741; C:mitochondrial outer membrane; TAS:Reactome.
 GO:0043531; F:ADP binding; IEA:Compara.
 GO:0016208; F:AMP binding; IEA:Compara.
 GO:0004128; F:cytochrome-b5 reductase activity, acting on NAD(P)H; TAS:Reactome.
 GO:0071949; F:FAD binding; IDA:UniProtKB.
 GO:0050660; F:flavin adenine dinucleotide binding; IEA:Compara.
 GO:0051287; F:NAD binding; IEA:Compara.
 GO:0008015; P:blood circulation; TAS:ProtInc.
 GO:0006695; P:cholesterol biosynthetic process; IEA:UniProtKB-KW.
 GO:0019852; P:L-ascorbic acid metabolic process; TAS:Reactome. 

Interpro

 IPR017927; Fd_Rdtase_FAD-bd.
 IPR001709; Flavoprot_Pyr_Nucl_cyt_Rdtase.
 IPR001834; NADH-Cyt_B5_reductase.
 IPR008333; OxRdtase_FAD-bd_dom.
 IPR001433; OxRdtase_FAD/NAD-bd.
 IPR017938; Riboflavin_synthase-like_b-brl. 

Pfam

 PF00970; FAD_binding_6
 PF00175; NAD_binding_1 

SMART

  

PROSITE

 PS51384; FAD_FR 

PRINTS

 PR00406; CYTB5RDTASE.
 PR00371; FPNCR.