CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-020849
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Isobutyryl-CoA dehydrogenase, mitochondrial 
Protein Synonyms/Alias
 Acyl-CoA dehydrogenase family member 8; ACAD-8 
Gene Name
 Acad8 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
48EEQKGFQKVAFDFAAacetylation[1]
68NMAEWDQKELFPVDVacetylation[1]
211RTGGSGAKGISCIVVacetylation[2]
211RTGGSGAKGISCIVVsuccinylation[2]
220ISCIVVEKGTPGLSFacetylation[1]
269QGFLIAMKGLNGGRIacetylation[1]
Reference
 [1] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [2] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337] 
Functional Description
 Has very high activity toward isobutyryl-CoA. Is an isobutyryl-CoA dehydrogenase that functions in valine catabolism (By similarity). 
Sequence Annotation
 NP_BIND 155 165 FAD (By similarity).
 NP_BIND 156 165 FAD (By similarity).
 NP_BIND 189 191 FAD (By similarity).
 NP_BIND 310 311 FAD; shared with dimeric partner (By
 NP_BIND 369 375 FAD (By similarity).
 NP_BIND 398 400 FAD (By similarity).
 REGION 272 275 Substrate binding (By similarity).
 ACT_SITE 396 396 Proton acceptor (By similarity).
 BINDING 165 165 Substrate; via carbonyl oxygen (By
 BINDING 300 300 FAD (By similarity).
 BINDING 300 300 FAD; shared with dimeric partner (By
 BINDING 397 397 Substrate; via amide nitrogen (By
 BINDING 408 408 Substrate (By similarity).  
Keyword
 Branched-chain amino acid catabolism; Complete proteome; FAD; Flavoprotein; Mitochondrion; Oxidoreductase; Reference proteome; Transit peptide. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 413 AA 
Protein Sequence
MAMLRSGYRR FGCLRAALKS LAQTHHRSIT FCIDPSLGLN EEQKGFQKVA FDFAAREMAP 60
NMAEWDQKEL FPVDVMRKAA QLGFGGVYVR TDVGGSGLSR LDTSVIFEAL ATGCTSTTAY 120
ISIHNMCAWM IDSFGNEEQR HKFCPPLCTM EKFASYCLTE PGSGSDAASL LTSAKQQGDH 180
YILNGSKAFI SGGGESDIYV VMCRTGGSGA KGISCIVVEK GTPGLSFGKK EKKVGWNSQP 240
TRAVIFEDCA VPVANRIGTE GQGFLIAMKG LNGGRINVAS CSLGAAHASV ILTQEHLKVR 300
KQFGAPLARS QYLQFQLADM ATKLVASRLM IRTAAVALQE EREDAVALCS MAKLFATEEC 360
FAICNQALQM HGGYGYLKDY AVQQYMRDSR VHQILEGSNE VMRMLISRNL LQD 413 
Gene Ontology
 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0003995; F:acyl-CoA dehydrogenase activity; IEA:Compara.
 GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
 GO:0006574; P:valine catabolic process; IEA:UniProtKB-UniPathway. 
Interpro
 IPR006089; Acyl-CoA_DH_CS.
 IPR006092; Acyl-CoA_DH_N.
 IPR006090; Acyl-CoA_Oxase/DH_1.
 IPR006091; Acyl-CoA_Oxase/DH_cen-dom.
 IPR009075; AcylCo_DH/oxidase_C.
 IPR013786; AcylCoA_DH/ox_N.
 IPR009100; AcylCoA_DH/oxidase. 
Pfam
 PF00441; Acyl-CoA_dh_1
 PF02770; Acyl-CoA_dh_M
 PF02771; Acyl-CoA_dh_N 
SMART
  
PROSITE
 PS00072; ACYL_COA_DH_1
 PS00073; ACYL_COA_DH_2 
PRINTS