CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000302
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Protein unc-13 homolog B 
Protein Synonyms/Alias
 Munc13-2; munc13 
Gene Name
 UNC13B 
Gene Synonyms/Alias
 UNC13 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
19KFQGSPDKFNTYVTLubiquitination[1]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473] 
Functional Description
 Plays a role in vesicle maturation during exocytosis as a target of the diacylglycerol second messenger pathway. Is involved in neurotransmitter release by acting in synaptic vesicle priming prior to vesicle fusion and participates in the activity- depending refilling of readily releasable vesicle pool (RRP). Essential for synaptic vesicle maturation in a subset of excitatory/glutamatergic but not inhibitory/GABA-mediated synapses (By similarity). 
Sequence Annotation
 DOMAIN 1 79 C2 1.
 DOMAIN 587 693 C2 2.
 DOMAIN 1013 1156 MHD1.
 DOMAIN 1263 1405 MHD2.
 DOMAIN 1425 1530 C2 3.
 ZN_FING 477 527 Phorbol-ester/DAG-type.  
Keyword
 Cell junction; Cell membrane; Coiled coil; Complete proteome; Cytoplasm; Exocytosis; Membrane; Metal-binding; Polymorphism; Reference proteome; Repeat; Synapse; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1591 AA 
Protein Sequence
MSLLCVRVKR AKFQGSPDKF NTYVTLKVQN VKSTTVAVRG DQPSWEQDFM FEISRLDLGL 60
SVEVWNKGLI WDTMVGTVWI ALKTIRQSDE EGPGEWSTLE AETLMKDDEI CGTRNPTPHK 120
ILLDTRFELP FDIPEEEARY WTYKWEQINA LGADNEYSSQ EESQRKPLPT AAAQCSFEDP 180
DSAVDDRDSD YRSETSNSFP PPYHTASQPN ASVHQFPVPV RSPQQLLLQG SSRDSCNDSM 240
QSYDLDYPER RAISPTSSSR YGSSCNVSQG SSQLSELDQY HEQDDDHRET DSIHSCHSSH 300
SLSRDGQAGF GEQEKPLEVT GQAEKEAACE PKEMKEDATT HPPPDLVLQK DHFLGPQESF 360
PEENASSPFT QARAHWIRAV TKVRLQLQEI PDDGDPSLPQ WLPEGPAGGL YGIDSMPDLR 420
RKKPLPLVSD LSLVQSRKAG ITSAMATRTS LKDEELKSHV YKKTLQALIY PISCTTPHNF 480
EVWTATTPTY CYECEGLLWG IARQGMRCSE CGVKCHEKCQ DLLNADCLQR AAEKSCKHGA 540
EDRTQNIIMA MKDRMKIRER NKPEIFEVIR DVFTVNKAAH VQQMKTVKQS VLDGTSKWSA 600
KITITVVCAQ GLQAKDKTGS SDPYVTVQVS KTKKRTKTIF GNLNPVWEEK FHFECHNSSD 660
RIKVRVWDED DDIKSRVKQR LKRESDDFLG QTIIEVRTLS GEMDVWYNLE KRTDKSAVSG 720
AIRLQISVEI KGEEKVAPYH VQYTCLHENL FHYLTDIQGS GGVRIPEARG DDAWKVYFDE 780
TAQEIVDEFA MRYGIESIYQ AMTHFACLSS KYMCPGVPAV MSTLLANINA YYAHTTASTN 840
VSASDRFAAS NFGKERFVKL LDQLHNSLRI DLSTYRNNFP AGSPERLQDL KSTVDLLTSI 900
TFFRMKVQEL QSPPRASQVV KDCVKACLNS TYEYIFNNCH DLYSRQYQLK QELPPEEQGP 960
SIRNLDFWPK LITLIVSIIE EDKNSYTPVL NQFPQELNVG KVSAEVMWHL FAQDMKYALE 1020
EHEKDHLCKS ADYMNLHFKV KWLHNEYVRD LPVLQGQVPE YPAWFEQFVL QWLDENEDVS 1080
LEFLRGALER DKKDGFQQTS EHALFSCSVV DVFTQLNQSF EIIRKLECPD PSILAHYMRR 1140
FAKTIGKVLM QYADILSKDF PAYCTKEKLP CILMNNVQQL RVQLEKMFEA MGGKELDLEA 1200
ADSLKELQVK LNTVLDELSM VFGNSFQVRI DECVRQMADI LGQVRGTGNA SPDARASAAQ 1260
DADSVLRPLM DFLDGNLTLF ATVCEKTVLK RVLKELWRVV MNTMERMIVL PPLTDQTGTQ 1320
LIFTAAKELS HLSKLKDHMV REETRNLTPK QCAVLDLALD TIKQYFHAGG NGLKKTFLEK 1380
SPDLQSLRYA LSLYTQTTDT LIKTFVRSQT TQGSGVDDPV GEVSIQVDLF THPGTGEHKV 1440
TVKVVAANDL KWQTAGMFRP FVEVTMVGPH QSDKKRKFTT KSKSNNWAPK YNETFHFLLG 1500
NEEGPESYEL QICVKDYCFA REDRVLGLAV MPLRDVTAKG SCACWCPLGR KIHMDETGLT 1560
ILRILSQRSN DEVAREFVKL KSESRSTEEG S 1591 
Gene Ontology
 GO:0030054; C:cell junction; IEA:UniProtKB-KW.
 GO:0005794; C:Golgi apparatus; TAS:ProtInc.
 GO:0031594; C:neuromuscular junction; IEA:Compara.
 GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
 GO:0019992; F:diacylglycerol binding; IEA:InterPro.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0004872; F:receptor activity; TAS:ProtInc.
 GO:0004871; F:signal transducer activity; TAS:ProtInc.
 GO:0007588; P:excretion; TAS:ProtInc.
 GO:0006917; P:induction of apoptosis; TAS:ProtInc.
 GO:0060384; P:innervation; IEA:Compara.
 GO:0035556; P:intracellular signal transduction; IEA:InterPro.
 GO:0007528; P:neuromuscular junction development; IEA:Compara.
 GO:0048172; P:regulation of short-term neuronal synaptic plasticity; IEA:Compara.
 GO:0035249; P:synaptic transmission, glutamatergic; IEA:Compara.
 GO:0016081; P:synaptic vesicle docking involved in exocytosis; IEA:Compara.
 GO:0016082; P:synaptic vesicle priming; IEA:Compara. 
Interpro
 IPR000008; C2_Ca-dep.
 IPR008973; C2_Ca/lipid-bd_dom_CaLB.
 IPR020477; C2_dom.
 IPR018029; C2_membr_targeting.
 IPR010439; Ca-dep_secretion_activator.
 IPR014770; Munc13_1.
 IPR014772; Munc13_dom-2.
 IPR019558; Munc13_subgr_dom-2.
 IPR002219; Prot_Kinase_C-like_PE/DAG-bd.
 IPR027080; Unc-13. 
Pfam
 PF00130; C1_1
 PF00168; C2
 PF06292; DUF1041
 PF10540; Membr_traf_MHD 
SMART
 SM00109; C1
 SM00239; C2 
PROSITE
 PS50004; C2
 PS51258; MHD1
 PS51259; MHD2
 PS00479; ZF_DAG_PE_1
 PS50081; ZF_DAG_PE_2 
PRINTS
 PR00360; C2DOMAIN.