CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023506
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Programmed cell death 6-interacting protein 
Protein Synonyms/Alias
 ALG-2-interacting protein 1; ALG-2-interacting protein X; E2F1-inducible protein; Eig2 
Gene Name
 Pdcd6ip 
Gene Synonyms/Alias
 Aip1; Alix 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
19TSEVDLAKPLVKFIQacetylation[1, 2, 3]
19TSEVDLAKPLVKFIQubiquitination[4]
23DLAKPLVKFIQQTYPubiquitination[4]
48RAAEELSKLRRSALGacetylation[1]
48RAAEELSKLRRSALGubiquitination[4]
60ALGRPLDKHEGALETacetylation[2]
60ALGRPLDKHEGALETubiquitination[4]
101TWKDAFDKGSLFGGSacetylation[1]
101TWKDAFDKGSLFGGSubiquitination[4]
215MKDAIIAKLANQAADacetylation[1]
229DYFGDAFKQCQYKDTacetylation[1]
234AFKQCQYKDTLPKEVacetylation[1]
285QHAAELIKNVASRYDubiquitination[4]
298YDEYVNVKDFSDKINubiquitination[4]
339IGKATLVKPTPVNVPacetylation[2]
350VNVPVSQKFTDLFEKubiquitination[4]
486TPSNDLYKPLRAEGAacetylation[1]
501KFRAVLDKAVQADGQubiquitination[4]
627KKQEGLLKNIQVSHQubiquitination[4]
638VSHQEFSKMKQSNNEubiquitination[4]
640HQEFSKMKQSNNEANubiquitination[4]
690ILVRFQNKCSDIVFAacetylation[1]
707TERDELLKDLQQSIAubiquitination[4]
Reference
 [1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [2] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [3] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [4] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Class E VPS protein involved in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome. Binds to the phospholipid lysobisphosphatidic acid (LBPA) which is abundant in MVBs internal membranes. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis. Appears to be an adapter for a subset of ESCRT-III proteins, such as CHMP4, to function at distinct membranes. Required for completion of cytokinesis. May play a role in the regulation of both apoptosis and cell proliferation. 
Sequence Annotation
 DOMAIN 3 392 BRO1.
 REGION 176 503 Interaction with CHMP4A, CHMP4B and
 REGION 503 869 Self-association (By similarity).
 REGION 717 720 Interaction with TSG101 (By similarity).
 REGION 798 807 Interaction with CEP55 (By similarity).
 MOD_RES 2 2 N-acetylalanine (By similarity).
 MOD_RES 215 215 N6-acetyllysine (By similarity).
 MOD_RES 730 730 Phosphoserine (By similarity).
 MOD_RES 741 741 Phosphothreonine (By similarity).  
Keyword
 Acetylation; Alternative splicing; Apoptosis; Cell cycle; Cell division; Complete proteome; Cytoplasm; Cytoskeleton; Phosphoprotein; Protein transport; Reference proteome; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 869 AA 
Protein Sequence
MASFIWVQLK KTSEVDLAKP LVKFIQQTYP SGGEEQAQYC RAAEELSKLR RSALGRPLDK 60
HEGALETLLR YYDQICSIEP KFPFSENQIC LTFTWKDAFD KGSLFGGSVK LALASLGYEK 120
SCVLFNCAAL ASQIAAEQNL DNDEGLKTAA KQYQFASGAF LHIKDTVLSA LSREPTVDIS 180
PDTVGTLSLI MLAQAQEVFF LKATRDKMKD AIIAKLANQA ADYFGDAFKQ CQYKDTLPKE 240
VFPTLAAKQC IMQANAEYHQ SILAKQQKKF GEEIARLQHA AELIKNVASR YDEYVNVKDF 300
SDKINRALTA AKKDNDFIYH DRVPDLKDLD PIGKATLVKP TPVNVPVSQK FTDLFEKMVP 360
VSVQQSLAVF SQRKADLVNR SIAQMREATT LANGVLASLN LPAAIEDVSG DTVPQSILTK 420
STSVVEQGGI QTVDQLIKEL PELLQRNREI LEESLRLLDE EEATDNDLRA KFKDRWQRTP 480
SNDLYKPLRA EGAKFRAVLD KAVQADGQVK ERYQSHRDTI ALLCKPEPEL NAAIPSANPA 540
KTMQGSEVVS VLKSLLSNLD EIKKERESLE NDLKSVNFDM TSKFLTALAQ DGVINEEALS 600
VTELDRIYGG LTSKVQESLK KQEGLLKNIQ VSHQEFSKMK QSNNEANLRE EVLKNLATAY 660
DNFVELVANL KEGTKFYNEL TEILVRFQNK CSDIVFARKT ERDELLKDLQ QSIAREPSAP 720
SIPPPAYQSS PAAGHAAAPP TPAPRTMPPA KPQPPARPPP PVLPANRVPP ASAAAAPAGV 780
GTASAAPPQT PGSAPPPQAQ GPPYPTYPGY PGYCQMPMPM GYNPYAYGQY NMPYPPVYHQ 840
SPGQAPYPGP QQPTYPFPQP PQQSYYPQQ 869 
Gene Ontology
 GO:0005829; C:cytosol; IDA:MGI.
 GO:0042470; C:melanosome; IEA:UniProtKB-SubCell.
 GO:0005815; C:microtubule organizing center; IEA:UniProtKB-SubCell.
 GO:0006915; P:apoptotic process; TAS:MGI.
 GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0015031; P:protein transport; IEA:UniProtKB-KW. 
Interpro
 IPR025304; ALIX_V_dom.
 IPR004328; BRO1_dom. 
Pfam
 PF13949; ALIX_LYPXL_bnd
 PF03097; BRO1 
SMART
 SM01041; BRO1 
PROSITE
 PS51180; BRO1 
PRINTS