UniProt Accession

 LEXA_ECOLI; P0A7C2; P03033; Q2M6R1 

Genbank Protein ID

 J01643; U00006; U00096; AP009048; L02362 

Genbank Nucleotide ID

 AAA24067.1; AAC43137.1; AAC77013.1; BAE78045.1; AAA24068.1 

Protein Name

 LexA repressor 

Protein Synonyms/Alias

  

Gene Name

 lexA 

Gene Synonyms/Alias

 exrA; spr; tsl; umuA; b4043; JW4003 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
48	NAAEEHLKALARKGV	acetylation	[1]
106	QVDPSLFKPNADFLL	acetylation	[1]
121	RVSGMSMKDIGIMDG	acetylation	[1]
135	GDLLAVHKTQDVRNG	acetylation	[1]
164	RLKKQGNKVELLPEN	acetylation	[1]
175	LPENSEFKPIVVDLR	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. Binds to the 16 bp palindromic sequence 5'-CTGTATATATATACAG-3'. In the presence of single-stranded DNA, RecA interacts with LexA causing an autocatalytic cleavage which disrupts the DNA-binding part of LexA, leading to derepression of the SOS regulon and eventually DNA repair. 

Sequence Annotation

 DNA_BIND 28 48 H-T-H motif.
 ACT_SITE 119 119 For autocatalytic cleavage activity (By
 ACT_SITE 156 156 For autocatalytic cleavage activity (By  

Keyword

 3D-structure; Autocatalytic cleavage; Complete proteome; DNA damage; DNA repair; DNA replication; DNA-binding; Hydrolase; Reference proteome; Repressor; SOS response; Transcription; Transcription regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 202 AA 

Protein Sequence

Gene Ontology

 GO:0003677; F:DNA binding; IDA:EcoliWiki.
 GO:0004252; F:serine-type endopeptidase activity; IEA:HAMAP.
 GO:0006281; P:DNA repair; IEA:HAMAP.
 GO:0006260; P:DNA replication; IEA:HAMAP.
 GO:0045892; P:negative regulation of transcription, DNA-dependent; IMP:EcoliWiki.
 GO:0006508; P:proteolysis; IEA:InterPro.
 GO:0006974; P:response to DNA damage stimulus; IMP:EcoliWiki.
 GO:0009432; P:SOS response; IMP:EcoCyc.
 GO:0006351; P:transcription, DNA-dependent; IDA:EcoCyc. 

Interpro

 IPR006199; LexA_DNA-bd_dom.
 IPR006200; Pept_S24_LexA.
 IPR006197; Peptidase_S24_LexA.
 IPR019759; Peptidase_S24_S26.
 IPR015927; Peptidase_S24_S26A/B/C.
 IPR011056; Peptidase_S24_S26A/B/C_b-rbn.
 IPR011991; WHTH_DNA-bd_dom. 

Pfam

 PF01726; LexA_DNA_bind
 PF00717; Peptidase_S24 

SMART

  

PROSITE

  

PRINTS

 PR00726; LEXASERPTASE.