CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-003160
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Glutamine synthetase 
Protein Synonyms/Alias
 Glutamate--ammonia ligase 
Gene Name
 glnA 
Gene Synonyms/Alias
 b3870; JW3841 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
16MLNEHEVKFVDLRFTacetylation[1]
48AEFFEEGKMFDGSSIacetylation[1]
177KGHRPAVKGGYFPVPacetylation[1]
240ADEIQIYKYVVHNVAacetylation[1]
252NVAHRFGKTATFMPKacetylation[1]
259KTATFMPKPMFGDNGacetylation[1]
277HCHMSLSKNGVNLFAacetylation[1]
287VNLFAGDKYAGLSEQacetylation[1]
304YYIGGVIKHAKAINAacetylation[1]
307GGVIKHAKAINALANacetylation[1]
321NPTTNSYKRLVPGYEacetylation[1]
353IPVVSSPKARRIEVRacetylation[1]
386GLDGIKNKIHPGEAMacetylation[1]
395HPGEAMDKNLYDLPPacetylation[1]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 
Functional Description
  
Sequence Annotation
 MOD_RES 398 398 O-AMP-tyrosine.  
Keyword
 ATP-binding; Complete proteome; Cytoplasm; Direct protein sequencing; Ligase; Nucleotide-binding; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 469 AA 
Protein Sequence
MSAEHVLTML NEHEVKFVDL RFTDTKGKEQ HVTIPAHQVN AEFFEEGKMF DGSSIGGWKG 60
INESDMVLMP DASTAVIDPF FADSTLIIRC DILEPGTLQG YDRDPRSIAK RAEDYLRSTG 120
IADTVLFGPE PEFFLFDDIR FGSSISGSHV AIDDIEGAWN SSTQYEGGNK GHRPAVKGGY 180
FPVPPVDSAQ DIRSEMCLVM EQMGLVVEAH HHEVATAGQN EVATRFNTMT KKADEIQIYK 240
YVVHNVAHRF GKTATFMPKP MFGDNGSGMH CHMSLSKNGV NLFAGDKYAG LSEQALYYIG 300
GVIKHAKAIN ALANPTTNSY KRLVPGYEAP VMLAYSARNR SASIRIPVVS SPKARRIEVR 360
FPDPAANPYL CFAALLMAGL DGIKNKIHPG EAMDKNLYDL PPEEAKEIPQ VAGSLEEALN 420
ELDLDREFLK AGGVFTDEAI DAYIALRREE DDRVRMTPHP VEFELYYSV 469 
Gene Ontology
 GO:0005829; C:cytosol; IDA:UniProtKB.
 GO:0016020; C:membrane; IDA:UniProtKB.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004356; F:glutamate-ammonia ligase activity; IDA:EcoCyc.
 GO:0019676; P:ammonia assimilation cycle; IDA:EcoCyc.
 GO:0006542; P:glutamine biosynthetic process; IEA:InterPro.
 GO:0009399; P:nitrogen fixation; IEA:InterPro.
 GO:0019740; P:nitrogen utilization; IDA:EcoCyc. 
Interpro
 IPR008147; Gln_synt_beta.
 IPR014746; Gln_synth/guanido_kin_cat_dom.
 IPR008146; Gln_synth_cat_dom.
 IPR027303; Gln_synth_gly_rich_site.
 IPR004809; Gln_synth_I.
 IPR001637; Gln_synth_I_adenylation_site.
 IPR027302; Gln_synth_N_conserv_site. 
Pfam
 PF00120; Gln-synt_C
 PF03951; Gln-synt_N 
SMART
  
PROSITE
 PS00180; GLNA_1
 PS00182; GLNA_ADENYLATION
 PS00181; GLNA_ATP 
PRINTS