UniProt Accession

 TANK_HUMAN; Q92844; D3DPB5; Q7Z4J6; Q92885 

Genbank Protein ID

 U59863; U63830; BT009855; AC009299; AC009313; CH471058; CH471058; CH471058; BC003388 

Genbank Nucleotide ID

 AAC50681.1; AAC50770.1; AAP88857.1; EAX11374.1; EAX11375.1; EAX11377.1; AAH03388.1 

Protein Name

 TRAF family member-associated NF-kappa-B activator 

Protein Synonyms/Alias

 TRAF-interacting protein; I-TRAF 

Gene Name

 TANK 

Gene Synonyms/Alias

 ITRAF; TRAF2 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
86	LEDSETRKNNLTLDQ	ubiquitination	[1]
186	VPIQCTDKTDKQEAL	ubiquitination	[2]
189	QCTDKTDKQEALFKP	ubiquitination	[1]
195	DKQEALFKPQAKDDI	ubiquitination	[1, 2, 3, 4]
199	ALFKPQAKDDINRGA	ubiquitination	[1, 2, 3, 4]
231	TSFESLSKFNVKFPP	ubiquitination	[1, 2, 3, 4, 5]
235	SLSKFNVKFPPMDND	ubiquitination	[2]
282	DNPGNFVKTEETLFE	sumoylation	[6]
282	DNPGNFVKTEETLFE	ubiquitination	[2]
304	ASAIQNLKTTDKTKP	ubiquitination	[2]
360	PSLDSPGKAIRGPQQ	ubiquitination	[2]
371	GPQQPIWKPFPNQDS	ubiquitination	[1, 2, 3, 4]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [6] Inducible SUMO modification of TANK alleviates its repression of TLR7 signalling.
 Renner F, Saul VV, Pagenstecher A, Wittwer T, Schmitz ML.
 EMBO Rep. 2011 Feb;12(2):129-35. [PMID: 21212807] 

Functional Description

 Acts as a regulator of TRAF function by maintaining them in a latent state. Overexpression inhibits TRAF2-mediated NF- kappa-B activation signaled by CD40, TNFR1 and TNFR2. Blocks TRAF2 binding to LMP1 and inhibits LMP1-mediated NF-kappa-B activation. May be involved in I-kappa-B-kinase (IKK) regulation; may function as an adapter for kinases such as TBK1 or IKBKE that can modulate IKK activity. 

Sequence Annotation

 ZN_FING 394 420 C2H2-type.
 REGION 172 191 TRAF family member interaction.
 MOD_RES 126 126 Phosphoserine.
 MOD_RES 129 129 Phosphoserine.
 MOD_RES 341 341 Phosphoserine.
 MOD_RES 354 354 Phosphoserine.
 MOD_RES 357 357 Phosphoserine.  

Keyword

 3D-structure; Alternative splicing; Complete proteome; Cytoplasm; Metal-binding; Phosphoprotein; Polymorphism; Reference proteome; Zinc; Zinc-finger. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 425 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0007249; P:I-kappaB kinase/NF-kappaB cascade; IEA:Compara.
 GO:0045087; P:innate immune response; TAS:Reactome.
 GO:0007165; P:signal transduction; TAS:ProtInc. 

Interpro

 IPR024581; TBD. 

Pfam

 PF12845; TBD 

SMART

  

PROSITE

 PS00028; ZINC_FINGER_C2H2_1
 PS50157; ZINC_FINGER_C2H2_2 

PRINTS