CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-011198
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Creatine kinase B-type 
Protein Synonyms/Alias
 B-CK; Creatine kinase B chain 
Gene Name
 Ckb 
Gene Synonyms/Alias
 Ckbb 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
107YQPSDEHKTDLNPDNubiquitination[1]
156GERRAIEKLAVEALSubiquitination[1]
196DDHFLFDKPVSPLLLubiquitination[1]
242LRVISMQKGGNMKEVubiquitination[1]
265TQIETLFKSKNYEFMubiquitination[1]
298LRAGVHIKLPHLGKHubiquitination[1]
304IKLPHLGKHEKFSEVubiquitination[1]
307PHLGKHEKFSEVLKRubiquitination[1]
313EKFSEVLKRLRLQKRubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023
Functional Description
 Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. 
Sequence Annotation
 DOMAIN 11 98 Phosphagen kinase N-terminal.
 DOMAIN 125 367 Phosphagen kinase C-terminal.
 NP_BIND 128 132 ATP (By similarity).
 NP_BIND 320 325 ATP (By similarity).
 BINDING 130 130 ATP (By similarity).
 BINDING 132 132 ATP (By similarity).
 BINDING 191 191 ATP (By similarity).
 BINDING 232 232 Substrate (By similarity).
 BINDING 236 236 ATP (By similarity).
 BINDING 285 285 Substrate (By similarity).
 BINDING 292 292 ATP (By similarity).
 BINDING 320 320 ATP (By similarity).
 BINDING 335 335 ATP (By similarity).
 MOD_RES 4 4 Phosphoserine (By similarity).
 MOD_RES 35 35 Phosphothreonine (By similarity).
 MOD_RES 39 39 Phosphotyrosine.
 MOD_RES 125 125 Phosphotyrosine.
 MOD_RES 164 164 Phosphoserine.
 MOD_RES 199 199 Phosphoserine (By similarity).
 MOD_RES 269 269 Nitrated tyrosine.  
Keyword
 ATP-binding; Complete proteome; Cytoplasm; Direct protein sequencing; Kinase; Nitration; Nucleotide-binding; Phosphoprotein; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 381 AA 
Protein Sequence
MPFSNSHNTQ KLRFPAEDEF PDLSSHNNHM AKVLTPELYA ELRAKCTPSG FTLDDAIQTG 60
VDNPGHPYIM TVGAVAGDEE SYDVFKDLFD PIIEERHGGY QPSDEHKTDL NPDNLQGGDD 120
LDPNYVLSSR VRTGRSIRGF CLPPHCSRGE RRAIEKLAVE ALSSLDGDLS GRYYALKSMT 180
EAEQQQLIDD HFLFDKPVSP LLLASGMARD WPDARGIWHN DNKTFLVWIN EEDHLRVISM 240
QKGGNMKEVF TRFCTGLTQI ETLFKSKNYE FMWNPHLGYI LTCPSNLGTG LRAGVHIKLP 300
HLGKHEKFSE VLKRLRLQKR GTGGVDTAAV GGVFDVSNAD RLGFSEVELV QMVVDGVKLL 360
IEMEQRLEQG QAIDDLMPAQ K 381 
Gene Ontology
 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004111; F:creatine kinase activity; IEA:EC.
 GO:0007420; P:brain development; IEA:Compara.
 GO:0030644; P:cellular chloride ion homeostasis; IEA:Compara. 
Interpro
 IPR000749; ATP-guanido_PTrfase.
 IPR022415; ATP-guanido_PTrfase_AS.
 IPR022414; ATP-guanido_PTrfase_cat.
 IPR022413; ATP-guanido_PTrfase_N.
 IPR014746; Gln_synth/guanido_kin_cat_dom. 
Pfam
 PF00217; ATP-gua_Ptrans
 PF02807; ATP-gua_PtransN 
SMART
  
PROSITE
 PS00112; PHOSPHAGEN_KINASE
 PS51510; PHOSPHAGEN_KINASE_C
 PS51509; PHOSPHAGEN_KINASE_N 
PRINTS