CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012521
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Serine/threonine-protein phosphatase 2A activator 
Protein Synonyms/Alias
 PP2A, subunit B', PR53 isoform; Phosphotyrosyl phosphatase activator; PTPA; Serine/threonine-protein phosphatase 2A regulatory subunit 4; Serine/threonine-protein phosphatase 2A regulatory subunit B' 
Gene Name
 PPP2R4 
Gene Synonyms/Alias
 PTPA 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
27TQNFIIPKKEIHTVPubiquitination[1]
62LNEGVKGKKLTFEYRacetylation[2]
63NEGVKGKKLTFEYRVubiquitination[3]
138QPSRFGNKAYRTWYAubiquitination[3]
277PRHFVDEKAVNENHKubiquitination[1]
321SAVPSWSKVNQGLIRubiquitination[3]
331QGLIRMYKAECLEKFacetylation[2]
331QGLIRMYKAECLEKFubiquitination[3]
337YKAECLEKFPVIQHFacetylation[2]
337YKAECLEKFPVIQHFubiquitination[3]
345FPVIQHFKFGSLLPIubiquitination[3]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961
Functional Description
 PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Acts as a regulatory subunit for serine/threonine- protein phosphatase 2A (PP2A) modulating its activity or substrate specificity, probably by inducing a conformational change in the catalytic subunit, a proposed direct target of the PPIase. Can reactivate inactive phosphatase PP2A-phosphatase methylesterase complexes (PP2A(i)) in presence of ATP and Mg(2+) (By similarity). Reversibly stimulates the variable phosphotyrosyl phosphatase activity of PP2A core heterodimer PP2A(D) in presence of ATP and Mg(2+) (in vitro). The phosphotyrosyl phosphatase activity is dependent of an ATPase activity of the PP2A(D):PPP2R4 complex. Is involved in apoptosis; the function appears to be independent from PP2A. 
Sequence Annotation
 NP_BIND 183 189 ATP.
 NP_BIND 240 242 ATP.
 NP_BIND 342 343 ATP.
 MOD_RES 2 2 N-acetylalanine.  
Keyword
 3D-structure; Acetylation; Alternative splicing; ATP-binding; Complete proteome; Cytoplasm; Direct protein sequencing; Isomerase; Nucleotide-binding; Nucleus; Polymorphism; Reference proteome; Rotamase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 358 AA 
Protein Sequence
MAEGERQPPP DSSEEAPPAT QNFIIPKKEI HTVPDMGKWK RSQAYADYIG FILTLNEGVK 60
GKKLTFEYRV SEMWNEVHEE KEQAAKQSVS CDECIPLPRA GHCAPSEAIE KLVALLNTLD 120
RWIDETPPVD QPSRFGNKAY RTWYAKLDEE AENLVATVVP THLAAAVPEV AVYLKESVGN 180
STRIDYGTGH EAAFAAFLCC LCKIGVLRVD DQIAIVFKVF NRYLEVMRKL QKTYRMEPAG 240
SQGVWGLDDF QFLPFIWGSS QLIDHPYLEP RHFVDEKAVN ENHKDYMFLE CILFITEMKT 300
GPFAEHSNQL WNISAVPSWS KVNQGLIRMY KAECLEKFPV IQHFKFGSLL PIHPVTSG 358 
Gene Ontology
 GO:0034704; C:calcium channel complex; IDA:BHF-UCL.
 GO:0005737; C:cytoplasm; IDA:UniProtKB.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0000159; C:protein phosphatase type 2A complex; TAS:HGNC.
 GO:0005524; F:ATP binding; IDA:HGNC.
 GO:0003755; F:peptidyl-prolyl cis-trans isomerase activity; IBA:RefGenome.
 GO:0046982; F:protein heterodimerization activity; TAS:HGNC.
 GO:0042803; F:protein homodimerization activity; IDA:HGNC.
 GO:0051721; F:protein phosphatase 2A binding; IDA:HGNC.
 GO:0008601; F:protein phosphatase type 2A regulator activity; IDA:HGNC.
 GO:0008160; F:protein tyrosine phosphatase activator activity; IDA:HGNC.
 GO:0006200; P:ATP catabolic process; IDA:GOC.
 GO:0030472; P:mitotic spindle organization in nucleus; IBA:RefGenome.
 GO:0032515; P:negative regulation of phosphoprotein phosphatase activity; IDA:HGNC.
 GO:0035308; P:negative regulation of protein dephosphorylation; IDA:HGNC.
 GO:0043065; P:positive regulation of apoptotic process; IDA:UniProtKB.
 GO:0032516; P:positive regulation of phosphoprotein phosphatase activity; IDA:HGNC.
 GO:0035307; P:positive regulation of protein dephosphorylation; IDA:HGNC.
 GO:0006457; P:protein folding; IEA:UniProtKB-KW. 
Interpro
 IPR004327; Phstyr_phstse_ac. 
Pfam
 PF03095; PTPA 
SMART
  
PROSITE
  
PRINTS