CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-003324
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Succinate dehydrogenase flavoprotein subunit 
Protein Synonyms/Alias
  
Gene Name
 sdhA 
Gene Synonyms/Alias
 b0723; JW0713 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
124RPFGGQSKNFGGEQAacetylation[1]
267EGGYLLNKHGERFMEacetylation[1, 2, 3]
281ERYAPNAKDLAGRDVacetylation[1]
312GPWGPHAKLKLDHLGacetylation[1, 3]
314WGPHAKLKLDHLGKEacetylation[1]
320LKLDHLGKEVLESRLacetylation[1, 3]
344FAHVDPVKEPIPVIPacetylation[1]
461EDPVAIRKALQECMQacetylation[3]
482REGDAMAKGLEQLKVacetylation[1]
488AKGLEQLKVIRERLKacetylation[1]
576RSVNMEPKLRPAFPPacetylation[1]
584LRPAFPPKIRTY***acetylation[1]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618]
 [2] Lysine acetylation is a highly abundant and evolutionarily conserved modification in Escherichia coli.
 Zhang J, Sprung R, Pei J, Tan X, Kim S, Zhu H, Liu CF, Grishin NV, Zhao Y.
 Mol Cell Proteomics. 2009 Feb;8(2):215-25. [PMID: 18723842]
 [3] Comprehensive profiling of protein lysine acetylation in Escherichia coli.
 Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z.
 J Proteome Res. 2013 Feb 1;12(2):844-51. [PMID: 23294111] 
Functional Description
 Two distinct, membrane-bound, FAD-containing enzymes are responsible for the catalysis of fumarate and succinate interconversion; the fumarate reductase is used in anaerobic growth, and the succinate dehydrogenase is used in aerobic growth. 
Sequence Annotation
 NP_BIND 14 19 FAD.
 NP_BIND 37 52 FAD.
 NP_BIND 404 405 FAD.
 ACT_SITE 286 286 Proton acceptor.
 BINDING 221 221 FAD.
 BINDING 242 242 Substrate.
 BINDING 254 254 Substrate.
 BINDING 354 354 Substrate.
 BINDING 388 388 FAD.
 BINDING 399 399 Substrate.
 MOD_RES 45 45 Tele-8alpha-FAD histidine.
 MOD_RES 267 267 N6-acetyllysine.  
Keyword
 3D-structure; Acetylation; Cell inner membrane; Cell membrane; Complete proteome; Direct protein sequencing; Electron transport; FAD; Flavoprotein; Membrane; Oxidoreductase; Reference proteome; Transport; Tricarboxylic acid cycle. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 588 AA 
Protein Sequence
MKLPVREFDA VVIGAGGAGM RAALQISQSG QTCALLSKVF PTRSHTVSAQ GGITVALGNT 60
HEDNWEWHMY DTVKGSDYIG DQDAIEYMCK TGPEAILELE HMGLPFSRLD DGRIYQRPFG 120
GQSKNFGGEQ AARTAAAADR TGHALLHTLY QQNLKNHTTI FSEWYALDLV KNQDGAVVGC 180
TALCIETGEV VYFKARATVL ATGGAGRIYQ STTNAHINTG DGVGMAIRAG VPVQDMEMWQ 240
FHPTGIAGAG VLVTEGCRGE GGYLLNKHGE RFMERYAPNA KDLAGRDVVA RSIMIEIREG 300
RGCDGPWGPH AKLKLDHLGK EVLESRLPGI LELSRTFAHV DPVKEPIPVI PTCHYMMGGI 360
PTKVTGQALT VNEKGEDVVV PGLFAVGEIA CVSVHGANRL GGNSLLDLVV FGRAAGLHLQ 420
ESIAEQGALR DASESDVEAS LDRLNRWNNN RNGEDPVAIR KALQECMQHN FSVFREGDAM 480
AKGLEQLKVI RERLKNARLD DTSSEFNTQR VECLELDNLM ETAYATAVSA NFRTESRGAH 540
SRFDFPDRDD ENWLCHSLYL PESESMTRRS VNMEPKLRPA FPPKIRTY 588 
Gene Ontology
 GO:0045282; C:plasma membrane succinate dehydrogenase complex; IDA:EcoliWiki.
 GO:0009055; F:electron carrier activity; IDA:EcoCyc.
 GO:0050660; F:flavin adenine dinucleotide binding; IDA:EcoliWiki.
 GO:0000104; F:succinate dehydrogenase activity; IMP:EcoliWiki.
 GO:0022900; P:electron transport chain; IEA:UniProtKB-KW.
 GO:0006099; P:tricarboxylic acid cycle; IGI:EcoliWiki. 
Interpro
 IPR003953; FAD_bind_dom.
 IPR013027; FAD_pyr_nucl-diS_OxRdtase.
 IPR003952; FRD_SDH_FAD_BS.
 IPR015939; Fum_Rdtase/Succ_DH_flav-like_C.
 IPR027477; Succ_DH/fumarate_Rdtase_cat.
 IPR011281; Succ_DH_flav_su_fwd.
 IPR014006; Succ_Dhase_FrdA_Gneg. 
Pfam
 PF00890; FAD_binding_2
 PF02910; Succ_DH_flav_C 
SMART
  
PROSITE
 PS00504; FRD_SDH_FAD_BINDING 
PRINTS
 PR00368; FADPNR.