CPLM-021573

Genbank Nucleotide ID

Chromatin modification-related protein MEAF6

Protein Synonyms/Alias

MYST/Esa1-associated factor 6; Esa1-associated factor 6 homolog; Protein EAF6 homolog; hEAF6; Sarcoma antigen NY-SAR-91

Homo sapiens (Human)

Position	Peptide	Type	References
6	**MAMHNKAAPPQIP	ubiquitination	[1]
24	RELAELVKRKQELAE	ubiquitination	[2]
26	LAELVKRKQELAETL	ubiquitination	[1, 2, 3]
69	DRYLTNQKNSNSKND	acetylation	[4]
74	NQKNSNSKNDRRNRK	acetylation	[4]
91	EAERLFSKSSVTSAA	acetylation	[4]
113	VQDQLIEKREPGSGT	ubiquitination	[5]

[1] Integrated proteomic analysis of post-translational modifications by serial enrichment.
Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
[2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
[4] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
[5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]

Functional Description

Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histone H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. Component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. Component of the MOZ/MORF complex which has a histone H3 acetyltransferase activity.

MOD_RES 69 69 N6-acetyllysine.
MOD_RES 74 74 N6-acetyllysine.
MOD_RES 118 118 Phosphoserine.
MOD_RES 120 120 Phosphothreonine.

Acetylation; Activator; Alternative splicing; Chromatin regulator; Chromosomal rearrangement; Coiled coil; Complete proteome; Nucleus; Phosphoprotein; Reference proteome; Transcription; Transcription regulation.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005813; C:centrosome; IDA:HPA.
GO:0005737; C:cytoplasm; IDA:HPA.
GO:0070776; C:MOZ/MORF histone acetyltransferase complex; IDA:UniProtKB.
GO:0035267; C:NuA4 histone acetyltransferase complex; IDA:UniProtKB.
GO:0005730; C:nucleolus; IDA:UniProtKB.
GO:0043968; P:histone H2A acetylation; IDA:UniProtKB.
GO:0044154; P:histone H3-K14 acetylation; IDA:UniProtKB.
GO:0043983; P:histone H4-K12 acetylation; IDA:UniProtKB.
GO:0043981; P:histone H4-K5 acetylation; IDA:UniProtKB.
GO:0043982; P:histone H4-K8 acetylation; IDA:UniProtKB.
GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.

IPR015418; Hist_AcTrfase_NuA4_cplx.