CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-024799
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ankyrin-3 
Protein Synonyms/Alias
 ANK-3; Ankyrin-G 
Gene Name
 Ank3 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
39ARAGHLEKALDYIKNubiquitination[1]
260TPLHVASKRGNANMVubiquitination[1]
946EELLVPSKEQHLTFTubiquitination[1]
1030CRLVKRHKLANPPPMubiquitination[1, 2]
1174FPEGALTKRIRVGLQubiquitination[1]
1197VKKILGNKATFSPIVubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113] 
Functional Description
 Membrane-cytoskeleton linker. May participate in the maintenance/targeting of ion channels and cell adhesion molecules at the nodes of Ranvier and axonal initial segments (By similarity). In skeletal muscle, required for costamere localization of DMD and betaDAG1. 
Sequence Annotation
 REPEAT 56 85 ANK 1.
 REPEAT 89 118 ANK 2.
 REPEAT 122 151 ANK 3.
 REPEAT 155 184 ANK 4.
 REPEAT 186 213 ANK 5.
 REPEAT 217 246 ANK 6.
 REPEAT 250 279 ANK 7.
 REPEAT 283 312 ANK 8.
 REPEAT 316 345 ANK 9.
 REPEAT 349 378 ANK 10.
 REPEAT 382 411 ANK 11.
 REPEAT 415 444 ANK 12.
 REPEAT 448 477 ANK 13.
 REPEAT 481 510 ANK 14.
 REPEAT 514 543 ANK 15.
 REPEAT 547 576 ANK 16.
 REPEAT 580 609 ANK 17.
 REPEAT 613 642 ANK 18.
 REPEAT 646 675 ANK 19.
 REPEAT 679 708 ANK 20.
 REPEAT 712 741 ANK 21.
 REPEAT 745 774 ANK 22.
 REPEAT 778 807 ANK 23.
 DOMAIN 983 1090 ZU5 1.
 DOMAIN 1140 1259 ZU5 2.
 DOMAIN 1478 1562 Death.
 REGION 1274 1408 UPA domain (By similarity).
 MOD_RES 22 22 Phosphoserine (By similarity).
 MOD_RES 830 830 Phosphoserine (By similarity).
 MOD_RES 1883 1883 Phosphoserine (By similarity).
 CROSSLNK 1923 1923 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 Alternative splicing; ANK repeat; Cell junction; Cell membrane; Complete proteome; Cytoplasm; Cytoskeleton; Isopeptide bond; Lysosome; Membrane; Phosphoprotein; Postsynaptic cell membrane; Reference proteome; Repeat; Synapse; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1961 AA 
Protein Sequence
MSEEPKEKPA KPAHRKRKGK KSDANASYLR AARAGHLEKA LDYIKNGVDV NICNQNGLNA 60
LHLASKEGHV EVVSELLQRE ANVDAATKKG NTALHIASLA GQAEVVKVLV TNGANVNAQS 120
QNGFTPLYMA AQENHLEVVR FLLDNGASQS LATEDGFTPL AVALQQGHDQ VVSLLLENDT 180
KGKVRLPALH IAARKDDTKA AALLLQNDTN ADVESKSGFT PLHIAAHYGN INVATLLLNR 240
AAAVDFTARN DITPLHVASK RGNANMVKLL LDRGAKIDAK TRDGLTPLHC GARSGHEQVV 300
EMLLDRSAPI LSKTKNGLSP LHMATQGDHL NCVQLLLQHN VPVDDVTNDY LTALHVAAHC 360
GHYKVAKVLL DKKASPNAKA LNGFTPLHIA CKKNRIRVME LLLKHGASIQ AVTESGLTPI 420
HVAAFMGHVN IVSQLMHHGA SPNTTNVRGE TALHMAARSG QAEVVRYLVQ DGAQVEAKAK 480
DDQTPLHISA RLGKADIVQQ LLQQGASPNA ATTSGYTPLH LAAREGHEDV AAFLLDHGAS 540
LSITTKKGFT PLHVAAKYGK LEVASLLLQK SASPDAAGKS GLTPLHVAAH YDNQKVALLL 600
LDQGASPHAA AKNGYTPLHI AAKKNQMDIA TSLLEYGADA NAVTRQGIAS VHLAAQEGHV 660
DMVSLLLSRN ANVNLSNKSG LTPLHLAAQE DRVNVAEVLV NQGAHVDAQT KMGYTPLHVG 720
CHYGNIKIVN FLLQHSAKVN AKTKNGYTAL HQAAQQGHTH IINVLLQNNA SPNELTVNGN 780
TALAIARRLG YISVVDTLKV VTEEIMTTTT ITEKHKMNVP ETMNEVLDMS DDEVRKASAP 840
EKLSDGEYIS DGEEGDKCTW FKIPKVQEVL VKSEDAITGD TDKYLGPQDL KELGDDSLPA 900
EGYVGFSLGA RSASLRSFSS DRSYTLNRSS YARDSMMIEE LLVPSKEQHL TFTREFDSDS 960
LRHYSWAADT LDNVNLVSSP VHSGFLVSFM VDARGGSMRG SRHHGMRIII PPRKCTAPTR 1020
ITCRLVKRHK LANPPPMVEG EGLASRLVEM GPAGAQFLGP VIVEIPHFGS MRGKERELIV 1080
LRSENGETWK EHQFDSKNED LAELLNGMDE ELDSPEELGT KRICRIITKD FPQYFAVVSR 1140
IKQESNQIGP EGGILSSTTV PLVQASFPEG ALTKRIRVGL QAQPVPEETV KKILGNKATF 1200
SPIVTVEPRR RKFHKPITMT IPVPPPSGEG VSNGYKGDAT PNLRLLCSIT GGTSPAQWED 1260
ITGTTPLTFI KDCVSFTTNV SARFWLADCH QVLETVGLAS QLYRELICVP YMAKFVVFAK 1320
TNDPVESSLR CFCMTDDRVD KTLEQQENFE EVARSKDIEV LEGKPIYVDC YGNLAPLTKG 1380
GQQLVFNFYS FKENRLPFSI KIRDTSQEPC GRLSFLKEPK TTKGLPQTAV CNLNITLPAH 1440
KKAEKADRRQ SFASLALRKR YSYLTEPSMS PQSPCERTDI RMAIVADHLG LSWTELAREL 1500
NFSVDEINQI RVENPNSLIS QSFMLLKKWV TRDGKNATTD ALTSVLTKIN RIDIVTLLEG 1560
PIFDYGNISG TRSFADENNV FHDPVDGHPS FQVELETPMG LYCTPPNPFQ QDDHFSDISS 1620
IESPFRTPSR LSDGLVPSQG NIEHPTGGPP VVTAEDTSLE DSKMDDSVTV TDPADPLDVD 1680
ESQLKDLCQS ECAQCWASVP GIPNDGRQAE PLRPQTRKVG MSSEQQEKGK SGPDEEVTED 1740
KVKSLFEDIQ LEEVEAEEMT EDQGQAMLNR VQRAELAMSS LAGWQNETPS GSLESPAQAR 1800
RLTGGLLDRL DDSSDQARDS ITSYLTGEPG KIEANGNHTA EVIPEAKAKP YFPESQNDIG 1860
KQSIKENLKP KTHGCGRTEE PVSPLTAYQK SLEETSKLVI EDAPKPCVPV GMKKMTRTTA 1920
DGKARLNLQE EEGSTRSEPK QGEGYKVKTK KEIRNVEKKT H 1961 
Gene Ontology
 GO:0043194; C:axon initial segment; IDA:BHF-UCL.
 GO:0009925; C:basal plasma membrane; IEA:Compara.
 GO:0005856; C:cytoskeleton; IEA:UniProtKB-SubCell.
 GO:0014704; C:intercalated disc; IDA:MGI.
 GO:0016328; C:lateral plasma membrane; IEA:Compara.
 GO:0005764; C:lysosome; IEA:UniProtKB-SubCell.
 GO:0016020; C:membrane; IDA:MGI.
 GO:0033268; C:node of Ranvier; IDA:BHF-UCL.
 GO:0033270; C:paranode region of axon; IDA:MGI.
 GO:0045211; C:postsynaptic membrane; IEA:UniProtKB-SubCell.
 GO:0042383; C:sarcolemma; IEA:UniProtKB-SubCell.
 GO:0045202; C:synapse; IDA:MGI.
 GO:0005923; C:tight junction; IEA:Compara.
 GO:0005200; F:structural constituent of cytoskeleton; IEA:Compara.
 GO:0007411; P:axon guidance; IMP:MGI.
 GO:0043001; P:Golgi to plasma membrane protein transport; IEA:Compara.
 GO:0072660; P:maintenance of protein location in plasma membrane; IEA:Compara.
 GO:0071709; P:membrane assembly; IEA:Compara.
 GO:0000281; P:mitotic cytokinesis; IEA:Compara.
 GO:0045760; P:positive regulation of action potential; TAS:BHF-UCL.
 GO:0072661; P:protein targeting to plasma membrane; IMP:BHF-UCL.
 GO:0019228; P:regulation of action potential in neuron; IMP:BHF-UCL.
 GO:0007165; P:signal transduction; IEA:InterPro.
 GO:0050808; P:synapse organization; IMP:MGI. 
Interpro
 IPR002110; Ankyrin_rpt.
 IPR020683; Ankyrin_rpt-contain_dom.
 IPR011029; DEATH-like_dom.
 IPR000488; Death_domain.
 IPR000906; ZU5. 
Pfam
 PF00023; Ank
 PF12796; Ank_2
 PF00531; Death
 PF00791; ZU5 
SMART
 SM00248; ANK
 SM00005; DEATH
 SM00218; ZU5 
PROSITE
 PS50297; ANK_REP_REGION
 PS50088; ANK_REPEAT
 PS50017; DEATH_DOMAIN
 PS51145; ZU5 
PRINTS
 PR01415; ANKYRIN.