UniProt Accession

 KITH_HUMAN; P04183; B2RC58; Q969V0; Q9UMG9 

Genbank Protein ID

 K02581; M15205; AK314950; BT006941; BC006484; BC007872; BC007986; M13643 

Genbank Nucleotide ID

 AAA61187.1; AAA61191.1; BAG37455.1; AAP35587.1; AAH06484.1; AAH07872.1; AAH07986.1; AAA61189.1 

Protein Name

 Thymidine kinase, cytosolic 

Protein Synonyms/Alias

  

Gene Name

 TK1 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
131	LDGTFQRKPFGAILN	ubiquitination	[1]
170	TKRLGTEKEVEVIGG	ubiquitination	[1]
220	GEAVAARKLFAPQQI	ubiquitination	[2, 3]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

  

Sequence Annotation

 NP_BIND 26 33 ATP.
 NP_BIND 58 60 ATP.
 NP_BIND 97 100 ATP.
 REGION 172 176 Substrate binding.
 ACT_SITE 98 98 Proton acceptor (Potential).
 METAL 153 153 Zinc.
 METAL 156 156 Zinc.
 METAL 185 185 Zinc.
 METAL 188 188 Zinc.
 BINDING 128 128 Substrate; via amide nitrogen.
 BINDING 181 181 Substrate.
 MOD_RES 2 2 N-acetylserine.
 MOD_RES 13 13 Phosphoserine.
 MOD_RES 231 231 Phosphoserine.  

Keyword

 3D-structure; Acetylation; ATP-binding; Complete proteome; Cytoplasm; DNA synthesis; Kinase; Metal-binding; Nucleotide-binding; Phosphoprotein; Reference proteome; Transferase; Zinc. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 234 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004797; F:thymidine kinase activity; TAS:ProtInc.
 GO:0008270; F:zinc ion binding; IDA:UniProtKB.
 GO:0048565; P:digestive tract development; IEA:Compara.
 GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
 GO:0060138; P:fetal process involved in parturition; IEA:Compara.
 GO:0001889; P:liver development; IEA:Compara.
 GO:0051289; P:protein homotetramerization; IPI:UniProtKB.
 GO:0006206; P:pyrimidine nucleobase metabolic process; TAS:Reactome.
 GO:0043097; P:pyrimidine nucleoside salvage; TAS:Reactome.
 GO:0046688; P:response to copper ion; IEA:Compara.
 GO:0051414; P:response to cortisol stimulus; IEA:Compara.
 GO:0031667; P:response to nutrient levels; IEA:Compara.
 GO:0009636; P:response to toxic substance; IEA:Compara.
 GO:0014856; P:skeletal muscle cell proliferation; IEA:Compara.
 GO:0046104; P:thymidine metabolic process; IEA:Compara. 

Interpro

 IPR027417; P-loop_NTPase.
 IPR001267; Thymidine_kinase.
 IPR020633; Thymidine_kinase_CS. 

Pfam

 PF00265; TK 

SMART

  

PROSITE

 PS00603; TK_CELLULAR_TYPE 

PRINTS