CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012322
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Enhancer of filamentation 1 
Protein Synonyms/Alias
 hEF1; CRK-associated substrate-related protein; CAS-L; CasL; Cas scaffolding protein family member 2; Neural precursor cell expressed developmentally down-regulated protein 9; NEDD-9; Renal carcinoma antigen NY-REN-12; p105; Enhancer of filamentation 1 p55 
Gene Name
 NEDD9 
Gene Synonyms/Alias
 CASL; CASS2 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
388STSSTSSKESSLSASubiquitination[1]
400SASPAQDKRLFLDPDubiquitination[1]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 Docking protein which plays a central coordinating role for tyrosine-kinase-based signaling related to cell adhesion. May function in transmitting growth control signals between focal adhesions at the cell periphery and the mitotic spindle in response to adhesion or growth factor signals initiating cell proliferation. May play an important role in integrin beta-1 or B cell antigen receptor (BCR) mediated signaling in B- and T-cells. Integrin beta-1 stimulation leads to recruitment of various proteins including CRK, NCK and SHPTP2 to the tyrosine phosphorylated form. 
Sequence Annotation
 DOMAIN 3 65 SH3.
 REGION 102 229 Interacts strongly with spindle-
 REGION 710 760 Divergent helix-loop-helix motif.
 MOTIF 360 363 Caspase cleavage related site.
 MOD_RES 92 92 Phosphotyrosine (By similarity).
 MOD_RES 177 177 Phosphotyrosine (By similarity).
 MOD_RES 189 189 Phosphotyrosine (By similarity).
 MOD_RES 296 296 Phosphoserine.
 MOD_RES 369 369 Phosphoserine.  
Keyword
 3D-structure; Alternative splicing; Cell adhesion; Cell cycle; Cell division; Cell junction; Cell projection; Complete proteome; Cytoplasm; Cytoskeleton; Golgi apparatus; Growth regulation; Mitosis; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; SH3 domain. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 834 AA 
Protein Sequence
MKYKNLMARA LYDNVPECAE ELAFRKGDIL TVIEQNTGGL EGWWLCSLHG RQGIVPGNRV 60
KLLIGPMQET ASSHEQPASG LMQQTFGQQK LYQVPNPQAA PRDTIYQVPP SYQNQGIYQV 120
PTGHGTQEQE VYQVPPSVQR SIGGTSGPHV GKKVITPVRT GHGYVYEYPS RYQKDVYDIP 180
PSHTTQGVYD IPPSSAKGPV FSVPVGEIKP QGVYDIPPTK GVYAIPPSAC RDEAGLREKD 240
YDFPPPMRQA GRPDLRPEGV YDIPPTCTKP AGKDLHVKYN CDIPGAAEPV ARRHQSLSPN 300
HPPPQLGQSV GSQNDAYDVP RGVQFLEPPA ETSEKANPQE RDGVYDVPLH NPPDAKGSRD 360
LVDGINRLSF SSTGSTRSNM STSSTSSKES SLSASPAQDK RLFLDPDTAI ERLQRLQQAL 420
EMGVSSLMAL VTTDWRCYGY MERHINEIRT AVDKVELFLK EYLHFVKGAV ANAACLPELI 480
LHNKMKRELQ RVEDSHQILS QTSHDLNECS WSLNILAINK PQNKCDDLDR FVMVAKTVPD 540
DAKQLTTTIN TNAEALFRPG PGSLHLKNGP ESIMNSTEYP HGGSQGQLLH PGDHKAQAHN 600
KALPPGLSKE QAPDCSSSDG SERSWMDDYD YVHLQGKEEF ERQQKELLEK ENIMKQNKMQ 660
LEHHQLSQFQ LLEQEITKPV ENDISKWKPS QSLPTTNSGV SAQDRQLLCF YYDQCETHFI 720
SLLNAIDALF SCVSSAQPPR IFVAHSKFVI LSAHKLVFIG DTLTRQVTAQ DIRNKVMNSS 780
NQLCEQLKTI VMATKMAALH YPSTTALQEM VHQVTDLSRN AQLFKRSLLE MATF 834 
Gene Ontology
 GO:0005938; C:cell cortex; IEA:UniProtKB-SubCell.
 GO:0005737; C:cytoplasm; TAS:ProtInc.
 GO:0005925; C:focal adhesion; IEA:UniProtKB-SubCell.
 GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.
 GO:0030027; C:lamellipodium; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; TAS:ProtInc.
 GO:0005819; C:spindle; TAS:ProtInc.
 GO:0000922; C:spindle pole; IEA:Compara.
 GO:0051017; P:actin filament bundle assembly; NAS:UniProtKB.
 GO:0007155; P:cell adhesion; NAS:ProtInc.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0007229; P:integrin-mediated signaling pathway; NAS:UniProtKB.
 GO:0007067; P:mitosis; IEA:UniProtKB-KW.
 GO:0040008; P:regulation of growth; IEA:UniProtKB-KW. 
Interpro
 IPR021901; CAS_DUF3513.
 IPR014928; Serine_rich.
 IPR001452; SH3_domain. 
Pfam
 PF12026; DUF3513
 PF08824; Serine_rich
 PF00018; SH3_1 
SMART
 SM00326; SH3 
PROSITE
 PS50002; SH3 
PRINTS