CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-009234
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Lipoyl synthase 
Protein Synonyms/Alias
 Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein LipA 
Gene Name
 lipA 
Gene Synonyms/Alias
 lip; b0628; JW0623 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
18VKYRDADKMALIPVKacetylation[1]
159CITAIREKSPQIKIEacetylation[1]
164REKSPQIKIETLVPDacetylation[1]
216ADYNWSLKLLERFKEacetylation[1]
316YHADLQAKGMEVK**acetylation[1]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 
Functional Description
 Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives. Free octanoate is not a substrate for LipA. 
Sequence Annotation
 METAL 68 68 Iron-sulfur 1 (4Fe-4S).
 METAL 73 73 Iron-sulfur 1 (4Fe-4S).
 METAL 79 79 Iron-sulfur 1 (4Fe-4S).
 METAL 94 94 Iron-sulfur 2 (4Fe-4S-S-AdoMet).
 METAL 98 98 Iron-sulfur 2 (4Fe-4S-S-AdoMet).
 METAL 101 101 Iron-sulfur 2 (4Fe-4S-S-AdoMet).  
Keyword
 4Fe-4S; Complete proteome; Cytoplasm; Iron; Iron-sulfur; Metal-binding; Reference proteome; S-adenosyl-L-methionine; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 321 AA 
Protein Sequence
MSKPIVMERG VKYRDADKMA LIPVKNVATE REALLRKPEW MKIKLPADST RIQGIKAAMR 60
KNGLHSVCEE ASCPNLAECF NHGTATFMIL GAICTRRCPF CDVAHGRPVA PDANEPVKLA 120
QTIADMALRY VVITSVDRDD LRDGGAQHFA DCITAIREKS PQIKIETLVP DFRGRMDRAL 180
DILTATPPDV FNHNLENVPR IYRQVRPGAD YNWSLKLLER FKEAHPEIPT KSGLMVGLGE 240
TNEEIIEVMR DLRRHGVTML TLGQYLQPSR HHLPVQRYVS PDEFDEMKAE ALAMGFTHAA 300
CGPFVRSSYH ADLQAKGMEV K 321 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:EcoliWiki.
 GO:0051539; F:4 iron, 4 sulfur cluster binding; IDA:EcoliWiki.
 GO:0016992; F:lipoate synthase activity; IDA:EcoliWiki.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0009249; P:protein lipoylation; IDA:EcoliWiki. 
Interpro
 IPR013785; Aldolase_TIM.
 IPR006638; Elp3/MiaB/NifB.
 IPR003698; Lipoyl_synth.
 IPR007197; rSAM. 
Pfam
 PF04055; Radical_SAM 
SMART
 SM00729; Elp3 
PROSITE
  
PRINTS