CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-003725
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Oligo-1,6-glucosidase IMA3 
Protein Synonyms/Alias
 Alpha-glucosidase; Isomaltase 3 
Gene Name
 IMA3 
Gene Synonyms/Alias
 YIL172C 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
366RFGDDSPKNRVISGKubiquitination[1]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 Alpha-glucosidase with broad substrate specificity for alpha-1,4- and alpha-1,6-glucosides. Not required for isomaltose utilization, but overexpression allows the IMA1 null mutant to grow on isomaltose. 
Sequence Annotation
 ACT_SITE 215 215 Nucleophile (By similarity).
 ACT_SITE 277 277 Proton donor (By similarity).  
Keyword
 Complete proteome; Cytoplasm; Glycosidase; Hydrolase; Maltose metabolism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 589 AA 
Protein Sequence
MTISSAHPET EPKWWKEATI YQIYPASFKD SNNDGWGDMK GIASKLEYIK ELGTDAIWIS 60
PFYDSPQDDM GYDIANYEKV WPTYGTNEDC FALIEKTHKL GMKFITDLVI NHCSSEHEWF 120
KESRSSKTNP KRDWFFWRPP KGYDAEGKPI PPNNWRSYFG GSAWTFDEKT QEFYLRLFCS 180
TQPDLNWENE DCRKAIYESA VGYWLDHGVD GFRIDVGSLY SKVAGLPDAP VIDENSKWQL 240
SDPFTMNGPR IHEFHQEMNK FIRNRVKDGR EIMTVGEMRH ATDETKRLYT SASRHELSEL 300
FNFSHTDVGT SPKFRQNLIP YELKDWKVAL AELFRYVNGT DCWSTIYLEN HDQPRSITRF 360
GDDSPKNRVI SGKLLSVLLV SLSGTLYVYQ GQELGEINFK NWPIEKYEDV EVRNNYDAIK 420
EEHGENSKEM KRFLEAIALI SRDHARTPMQ WSREEPNAGF SGPNAKPWFY LNESFREGIN 480
AEDESKDPNS VLNFWKEALR FRKAHKDITV YGYDFEFIDL DNKKLFSFTK KYDNKTLFAA 540
LNFSSDSIDF TIPNNSSSFK LEFGNYPRSE VDASSRTLKP WEGRIYISE 589 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:SGD.
 GO:0043169; F:cation binding; IEA:InterPro.
 GO:0004574; F:oligo-1,6-glucosidase activity; IDA:SGD.
 GO:0046352; P:disaccharide catabolic process; IGI:SGD.
 GO:0000023; P:maltose metabolic process; IEA:UniProtKB-KW. 
Interpro
 IPR015902; Glyco_hydro_13.
 IPR013780; Glyco_hydro_13_b.
 IPR006047; Glyco_hydro_13_cat_dom.
 IPR006589; Glyco_hydro_13_sub_cat_dom.
 IPR013781; Glyco_hydro_catalytic_dom.
 IPR017853; Glycoside_hydrolase_SF. 
Pfam
 PF00128; Alpha-amylase 
SMART
 SM00642; Aamy 
PROSITE
  
PRINTS