CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-016625
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Succinate-semialdehyde dehydrogenase, mitochondrial 
Protein Synonyms/Alias
 Aldehyde dehydrogenase family 5 member A1; NAD(+)-dependent succinic semialdehyde dehydrogenase 
Gene Name
 Aldh5a1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
74YDPASGAKLGTVADCacetylation[1, 2]
114ERSLLLRKWYDLMIQacetylation[1, 3, 4]
114ERSLLLRKWYDLMIQsuccinylation[4]
123YDLMIQNKDDLAKIIacetylation[1, 2, 3, 4, 5]
123YDLMIQNKDDLAKIIsuccinylation[4]
128QNKDDLAKIITAESGacetylation[1, 2, 3, 5]
136IITAESGKPLKEAQGacetylation[6]
172DIIYTSAKDKRGLVLacetylation[1, 2, 4, 5]
172DIIYTSAKDKRGLVLsuccinylation[4]
174IYTSAKDKRGLVLKQacetylation[1]
253PCSRNKAKEVGEVLCacetylation[1, 3, 4, 5]
253PCSRNKAKEVGEVLCsuccinylation[4]
320VAGAMASKFRNAGQTacetylation[1]
347IHDSFVTKFAEAMKKacetylation[1, 2, 4]
347IHDSFVTKFAEAMKKsuccinylation[4]
353TKFAEAMKKSLRVGNacetylation[1]
375QGPLINEKAVEKVEKacetylation[2, 5]
379INEKAVEKVEKQVNDacetylation[5]
382KAVEKVEKQVNDAVAacetylation[2, 3, 5]
390QVNDAVAKGATVVTGacetylation[4]
390QVNDAVAKGATVVTGsuccinylation[4]
399ATVVTGGKRHQSGGNacetylation[1]
Reference
 [1] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
 [2] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [3] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
 [4] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [5] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [6] Mitochondrial acetylome analysis in a mouse model of alcohol-induced liver injury utilizing SIRT3 knockout mice.
 Fritz KS, Galligan JJ, Hirschey MD, Verdin E, Petersen DR.
 J Proteome Res. 2012 Mar 2;11(3):1633-43. [PMID: 22309199] 
Functional Description
 Catalyzes one step in the degradation of the inhibitory neurotransmitter gamma-aminobutyric acid (GABA) (By similarity). 
Sequence Annotation
 NP_BIND 216 219 NAD (By similarity).
 NP_BIND 272 277 NAD (By similarity).
 ACT_SITE 294 294 Proton acceptor (By similarity).
 ACT_SITE 328 328 Nucleophile (By similarity).
 BINDING 201 201 NAD (By similarity).
 BINDING 201 201 Substrate (By similarity).
 BINDING 322 322 Substrate (By similarity).
 BINDING 486 486 Substrate (By similarity).
 MOD_RES 67 67 Phosphotyrosine.
 MOD_RES 114 114 N6-acetyllysine (By similarity).
 DISULFID 328 330 In inhibited form (By similarity).  
Keyword
 Acetylation; Complete proteome; Direct protein sequencing; Disulfide bond; Mitochondrion; NAD; Oxidoreductase; Phosphoprotein; Reference proteome; Transit peptide. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 523 AA 
Protein Sequence
MATCFLLRSF WAARPALPPP GRFRPEPAGT PRRSYASGPG GLHADLLRGD SFVGGRWLPA 60
PATFPVYDPA SGAKLGTVAD CGVPEARAAV RAAYDAFNSW KGVSVKERSL LLRKWYDLMI 120
QNKDDLAKII TAESGKPLKE AQGEILYSAL FLEWFSEEAR RIYGDIIYTS AKDKRGLVLK 180
QPVGVAAIIT PWNFPSAMIT RKVGAALAAG CTVVVKPAED TPYSALALAQ LANQAGIPAG 240
VYNVIPCSRN KAKEVGEVLC TDPLVSKISF TGSTATGKIL LHHAANSVKR VSMELGGLAP 300
FIVFDSANVD QAVAGAMASK FRNAGQTCVC SNRFLVQRGI HDSFVTKFAE AMKKSLRVGN 360
GFEEGTTQGP LINEKAVEKV EKQVNDAVAK GATVVTGGKR HQSGGNFFEP TLLSNVTRDM 420
LCITEETFGP LAPVIKFDKE EEAVAIANAA EVGLAGYFYS QDPAQIWRVA EQLEVGMVGV 480
NEGLISSVEC PFGGVKQSGL GREGSKYGID EYLEVKYVCY GGL 523 
Gene Ontology
 GO:0005739; C:mitochondrion; IDA:UniProtKB.
 GO:0004777; F:succinate-semialdehyde dehydrogenase (NAD+) activity; ISS:UniProtKB.
 GO:0009013; F:succinate-semialdehyde dehydrogenase [NAD(P)+] activity; IEA:InterPro.
 GO:0006083; P:acetate metabolic process; IMP:MGI.
 GO:0007417; P:central nervous system development; ISS:UniProtKB.
 GO:0006681; P:galactosylceramide metabolic process; IMP:MGI.
 GO:0009450; P:gamma-aminobutyric acid catabolic process; ISS:UniProtKB.
 GO:0006006; P:glucose metabolic process; IMP:MGI.
 GO:0006678; P:glucosylceramide metabolic process; IMP:MGI.
 GO:0006536; P:glutamate metabolic process; IMP:MGI.
 GO:0006541; P:glutamine metabolic process; IMP:MGI.
 GO:0006749; P:glutathione metabolic process; IMP:MGI.
 GO:0006650; P:glycerophospholipid metabolic process; IMP:MGI.
 GO:0042135; P:neurotransmitter catabolic process; IMP:MGI.
 GO:0009791; P:post-embryonic development; IMP:MGI.
 GO:0051289; P:protein homotetramerization; IEA:Compara.
 GO:0022904; P:respiratory electron transport chain; IMP:MGI.
 GO:0046459; P:short-chain fatty acid metabolic process; IMP:MGI.
 GO:0006105; P:succinate metabolic process; IMP:MGI. 
Interpro
 IPR016161; Ald_DH/histidinol_DH.
 IPR016163; Ald_DH_C.
 IPR016160; Ald_DH_CS.
 IPR016162; Ald_DH_N.
 IPR015590; Aldehyde_DH_dom.
 IPR010102; Succ_semiAld_DH. 
Pfam
 PF00171; Aldedh 
SMART
  
PROSITE
 PS00070; ALDEHYDE_DEHYDR_CYS
 PS00687; ALDEHYDE_DEHYDR_GLU 
PRINTS