UniProt Accession

 FMT_ECOLI; P23882; P77040; Q2M6V2 

Genbank Protein ID

 X63666; X77091; U18997; U00096; AP009048; X00767; Y10307 

Genbank Nucleotide ID

 CAA45207.1; CAA54368.1; AAA58085.1; AAC76313.1; BAE78004.1; CAA25339.1; CAA71358.1 

Protein Name

 Methionyl-tRNA formyltransferase 

Protein Synonyms/Alias

  

Gene Name

 fmt 

Gene Synonyms/Alias

 yhdD; b3288; JW3249 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
193	QLADGTAKPEVQDET	acetylation	[1]
207	TLVTYAEKLSKEEAR	acetylation	[1]
210	TYAEKLSKEEARIDW	acetylation	[1, 2]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618]
 [2] Comprehensive profiling of protein lysine acetylation in Escherichia coli.
 Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z.
 J Proteome Res. 2013 Feb 1;12(2):844-51. [PMID: 23294111] 

Functional Description

 Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by: (I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-GTP. 

Sequence Annotation

 REGION 2 189 N-terminal domain.
 REGION 113 116 Tetrahydrofolate (THF) binding.
 REGION 210 315 C-terminal domain.  

Keyword

 3D-structure; Complete proteome; Direct protein sequencing; Protein biosynthesis; Reference proteome; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 315 AA 

Protein Sequence

Gene Ontology

 GO:0004479; F:methionyl-tRNA formyltransferase activity; IDA:EcoCyc.
 GO:0008168; F:methyltransferase activity; IEA:InterPro.
 GO:0032259; P:methylation; IEA:GOC. 

Interpro

 IPR005794; Fmt.
 IPR005793; Formyl_trans_C.
 IPR002376; Formyl_transf_N.
 IPR011034; Formyl_transferase_C-like.
 IPR001555; GART_AS.
 IPR015518; Met_tRNA_Form_TA-like. 

Pfam

 PF02911; Formyl_trans_C
 PF00551; Formyl_trans_N 

SMART

  

PROSITE

 PS00373; GART 

PRINTS