CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-018286
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 DDB1- and CUL4-associated factor 4 
Protein Synonyms/Alias
 WD repeat-containing protein 21A 
Gene Name
 DCAF4 
Gene Synonyms/Alias
 WDR21; WDR21A 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
208IINLQSLKTPTLKVFubiquitination[1]
460PSPYPASKADIPSVAubiquitination[2]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 May function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex. 
Sequence Annotation
 REPEAT 368 407 WD 1.
 REPEAT 410 451 WD 2.  
Keyword
 3D-structure; Alternative splicing; Complete proteome; Polymorphism; Reference proteome; Repeat; Ubl conjugation pathway; WD repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 495 AA 
Protein Sequence
MNKSRWQSRR RHGRRSHQQN PWFRLRDSED RSDSRAAQPA HDSGHGDDES PSTSSGTAGT 60
SSVPELPGFY FDPEKKRYFR LLPGHNNCNP LTKESIRQKE MESKRLRLLQ EEDRRKKIAR 120
MGFNASSMLR KSQLGFLNVT NYCHLAHELR LSCMERKKVQ IRSMDPSALA SDRFNLILAD 180
TNSDRLFTVN DVKVGGSKYG IINLQSLKTP TLKVFMHENL YFTNRKVNSV CWASLNHLDS 240
HILLCLMGLA ETPGCATLLP ASLFVNSHPG IDRPGMLCSF RIPGAWSCAW SLNIQANNCF 300
STGLSRRVLL TNVVTGHRQS FGTNSDVLAQ QFALMAPLLF NGCRSGEIFA IDLRCGNQGK 360
GWKATRLFHD SAVTSVRILQ DEQYLMASDM AGKIKLWDLR TTKCVRQYEG HVNEYAYLPL 420
HVHEEEGILV AVGQDCYTRI WSLHDARLLR TIPSPYPASK ADIPSVAFSS RLGGSRGAPG 480
LLMAVGQDLY CYSYS 495 
Gene Ontology
 GO:0080008; C:Cul4-RING ubiquitin ligase complex; IDA:UniProtKB.
 GO:0016567; P:protein ubiquitination; IC:UniProtKB. 
Interpro
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR017986; WD40_repeat_dom. 
Pfam
 PF00400; WD40 
SMART
 SM00320; WD40 
PROSITE
 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 
PRINTS