CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-005436
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase 
Protein Synonyms/Alias
 Cobalamin-independent methionine synthase; Methionine synthase, vitamin-B12 independent isozyme 
Gene Name
 metE 
Gene Synonyms/Alias
 b3829; JW3805 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
20VGLRRELKKAQESYWacetylation[1]
21GLRRELKKAQESYWAacetylation[1]
87VPARHQNKDGSVDIDacetylation[1]
117AAAAEMTKWFNTNYHacetylation[1]
132YMVPEFVKGQQFKLTacetylation[1]
158LALGHKVKPVLLGPVacetylation[1]
172VTWLWLGKVKGEQFDacetylation[1]
174WLWLGKVKGEQFDRLacetylation[1]
270HVDLVHGKDDVAELHacetylation[1]
278DDVAELHKRLPSDWLacetylation[1]
304WRADLTEKYAQIKDIacetylation[1]
309TEKYAQIKDIVGKRDacetylation[1]
314QIKDIVGKRDLWVASacetylation[1]
394VHNPAVEKRLAAITAacetylation[1]
422EAQRARFKLPAWPTTacetylation[1]
448RTLRLDFKKGNLDANacetylation[1]
449TLRLDFKKGNLDANNacetylation[1]
466TGIAEHIKQAIVEQEacetylation[1]
518SYGSRCVKPPIVIGDacetylation[1]
538PITVEWAKYAQSLTDacetylation[1]
546YAQSLTDKPVKGMLTacetylation[1]
729VNPDCGLKTRGWPETacetylation[1]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 
Functional Description
 Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation. 
Sequence Annotation
 REPEAT 2 370 Approximate.
 REPEAT 371 753 Approximate.
 METAL 641 641 Zinc.
 METAL 643 643 Zinc.
 METAL 726 726 Zinc.  
Keyword
 Amino-acid biosynthesis; Complete proteome; Direct protein sequencing; Metal-binding; Methionine biosynthesis; Methyltransferase; Reference proteome; Repeat; Transferase; Zinc. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 753 AA 
Protein Sequence
MTILNHTLGF PRVGLRRELK KAQESYWAGN STREELLAVG RELRARHWDQ QKQAGIDLLP 60
VGDFAWYDHV LTTSLLLGNV PARHQNKDGS VDIDTLFRIG RGRAPTGEPA AAAEMTKWFN 120
TNYHYMVPEF VKGQQFKLTW TQLLDEVDEA LALGHKVKPV LLGPVTWLWL GKVKGEQFDR 180
LSLLNDILPV YQQVLAELAK RGIEWVQIDE PALVLELPQA WLDAYKPAYD ALQGQVKLLL 240
TTYFEGVTPN LDTITALPVQ GLHVDLVHGK DDVAELHKRL PSDWLLSAGL INGRNVWRAD 300
LTEKYAQIKD IVGKRDLWVA SSCSLLHSPI DLSVETRLDA EVKSWFAFAL QKCHELALLR 360
DALNSGDTAA LAEWSAPIQA RRHSTRVHNP AVEKRLAAIT AQDSQRANVY EVRAEAQRAR 420
FKLPAWPTTT IGSFPQTTEI RTLRLDFKKG NLDANNYRTG IAEHIKQAIV EQERLGLDVL 480
VHGEAERNDM VEYFGEHLDG FVFTQNGWVQ SYGSRCVKPP IVIGDISRPA PITVEWAKYA 540
QSLTDKPVKG MLTGPVTILC WSFPREDVSR ETIAKQIALA LRDEVADLEA AGIGIIQIDE 600
PALREGLPLR RSDWDAYLQW GVEAFRINAA VAKDDTQIHT HMCYCEFNDI MDSIAALDAD 660
VITIETSRSD MELLESFEEF DYPNEIGPGV YDIHSPNVPS VEWIEALLKK AAKRIPAERL 720
WVNPDCGLKT RGWPETRAAL ANMVQAAQNL RRG 753 
Gene Ontology
 GO:0003871; F:5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity; IDA:EcoCyc.
 GO:0008270; F:zinc ion binding; IPI:EcoCyc.
 GO:0009086; P:methionine biosynthetic process; IEA:HAMAP. 
Interpro
 IPR013215; Cbl-indep_Met_Synth_N.
 IPR006276; Cobalamin-indep_Met_synthase.
 IPR002629; Methionine_synth. 
Pfam
 PF08267; Meth_synt_1
 PF01717; Meth_synt_2 
SMART
  
PROSITE
  
PRINTS