CPLM-016728

Genbank Nucleotide ID

NAD kinase 2, mitochondrial

Protein Synonyms/Alias

Mitochondrial NAD kinase; NAD kinase domain-containing protein 1, mitochondrial

Mus musculus (Mouse)

Position	Peptide	Type	References
64	SRVVVVAKTTRYEFE	acetylation	[1, 2, 3, 4]
64	SRVVVVAKTTRYEFE	succinylation	[4]
86	ELSEEDLKQLLALKG	acetylation	[3]
86	ELSEEDLKQLLALKG	ubiquitination	[5]
92	LKQLLALKGSSYSGL	ubiquitination	[5]
108	ERHHIHTKNVEHIID	acetylation	[3]
312	VDDGPWEKQKSSGLN	acetylation	[4, 6]
312	VDDGPWEKQKSSGLN	succinylation	[4]
314	DGPWEKQKSSGLNLC	acetylation	[4, 6]
314	DGPWEKQKSSGLNLC	succinylation	[4]
314	DGPWEKQKSSGLNLC	ubiquitination	[5]
327	LCTGTGSKAWSFNIN	acetylation	[3, 4]
327	LCTGTGSKAWSFNIN	succinylation	[4]
360	NLTLPLNKDLVEKVT	acetylation	[6, 7]
365	LNKDLVEKVTNEYNE	acetylation	[6]
407	RQRCFSSKVCVRSRC	acetylation	[1, 3, 6, 7]

[1] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
Simon GM, Cheng J, Gordon JI.
Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
[2] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
[3] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
[4] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
[5] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
[6] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
[7] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]

Functional Description

Mitochondrial NAD(+) kinase that phosphorylates NAD(+) to yield NADP(+). Can use both ATP or inorganic polyphosphate as the phosphoryl donor (By similarity).

MOD_RES 176 176 Phosphoserine.

Alternative splicing; ATP-binding; Complete proteome; Kinase; Mitochondrion; NAD; NADP; Nucleotide-binding; Phosphoprotein; Reference proteome; Transferase; Transit peptide.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005739; C:mitochondrion; IDA:MGI.
GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
GO:0003951; F:NAD+ kinase activity; ISS:UniProtKB.
GO:0042803; F:protein homodimerization activity; ISS:UniProtKB.
GO:0019674; P:NAD metabolic process; ISS:UniProtKB.
GO:0006741; P:NADP biosynthetic process; IEA:InterPro.

IPR012355; ATP-NAD-like_euk.
IPR017438; ATP-NAD_kinase_dom_1.
IPR016064; ATP-NAD_kinase_PpnK-typ.
IPR017437; ATP-NAD_kinase_PpnK-typ_all-b.
IPR002504; PolyP/ATP_NADK_prd.