CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-039701
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Centrosomal protein of 57 kDa 
Protein Synonyms/Alias
  
Gene Name
 CEP57 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
47RAIFSALKNLQDKIRubiquitination[1]
52ALKNLQDKIRRLELEubiquitination[1, 2]
68IQAEESVKTLSRETIubiquitination[1, 2, 3, 4]
79RETIEYKKVLDEQIQubiquitination[1, 2]
92IQERENSKNEESKHNubiquitination[1]
118NKCNLLEKQLEYMRNubiquitination[1]
128EYMRNMIKHAEMERTubiquitination[1, 2]
140ERTSVLEKQVSLEREubiquitination[1, 2, 3, 5]
171LLEQEYNKLTTMQALubiquitination[1]
182MQALAEKKMQELEAKubiquitination[1]
203ERKRMQAKAAELQTGubiquitination[1, 2]
221NRLIFEDKATPCVPNubiquitination[1, 2, 4]
307INSIPLAKQVSSRGGubiquitination[1, 2]
369PTVELKDKLECELEAubiquitination[1, 2]
384LVGRMEAKANQITKVubiquitination[1]
390AKANQITKVRKYQAQubiquitination[1]
435GITGTTNKKDFMKLRubiquitination[1]
449RPGEKRRKNLQLLKDubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 473 AA 
Protein Sequence
MVRHSSSPYV VYPSDKPFLN SDLRRSPSKP TLAYPESNSR AIFSALKNLQ DKIRRLELER 60
IQAEESVKTL SRETIEYKKV LDEQIQEREN SKNEESKHNQ ELTSQLLAAE NKCNLLEKQL 120
EYMRNMIKHA EMERTSVLEK QVSLERERQH DQTHVQSQLE KLDLLEQEYN KLTTMQALAE 180
KKMQELEAKL HEEEQERKRM QAKAAELQTG LETNRLIFED KATPCVPNAR RIKKKKSKPP 240
EKKSSRNYFG AQPHYRLCLG DMPFVAGKST SPSHAVVANV QLVLHLMKQH SKALCNDRVI 300
NSIPLAKQVS SRGGKSKKLS VTPPSSNGIN EELSEVLQTL QDEFGQMSFD HQQLAKLIQE 360
SPTVELKDKL ECELEALVGR MEAKANQITK VRKYQAQLEK QKLEKQKKEL KATKKTLDEE 420
RNSSSRSGIT GTTNKKDFMK LRPGEKRRKN LQLLKDMQSI QNSLQSSSLC WDY 473 
Gene Ontology
 GO:0005813; C:centrosome; IEA:Compara.
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0015630; C:microtubule cytoskeleton; IDA:HPA.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0034453; P:microtubule anchoring; IEA:InterPro.
 GO:0051260; P:protein homooligomerization; IEA:Compara.
 GO:0000060; P:protein import into nucleus, translocation; IEA:Compara.
 GO:0007286; P:spermatid development; IEA:Compara. 
Interpro
 IPR010597; Centrosomal_protein_57kDa.
 IPR025913; Cep57_CLD.
 IPR024957; Cep57_MT-bd_dom. 
Pfam
 PF14073; Cep57_CLD
 PF06657; Cep57_MT_bd 
SMART
  
PROSITE
  
PRINTS