CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-031546
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Methyltransferase-like protein 13 
Protein Synonyms/Alias
 cDNA FLJ56024 
Gene Name
 METTL13 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
18GSVDYWEKFFQQRGKubiquitination[1, 2, 3, 4]
50KYIKPREKVLVIGCGubiquitination[3]
133VLTDEEEKTLQQVDRubiquitination[1, 2, 3, 4]
168AQAHILKKAVGHFSRubiquitination[3]
231LCAQEQRKPVRLESAubiquitination[3]
292VVDSPTVKPSRDNHFubiquitination[1, 2, 3, 4, 5]
321FGMDEGRKQLAASAGubiquitination[3, 6]
407EDVQGDDKRYFRRLIubiquitination[2, 3]
430QSEARLLKDVSHKAQubiquitination[3]
465APGQSIDKSYLCCEHubiquitination[3]
622VCRDLGLKDSVLAGLubiquitination[1, 3]
693YVLSDMLKTVKIV**ubiquitination[3]
696SDMLKTVKIV*****ubiquitination[3]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [6] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 698 AA 
Protein Sequence
MRAFVVSREF GSVDYWEKFF QQRGKKAFEW YGTYLELCGV LHKYIKPREK VLVIGCGNSE 60
LSEQLYDVGY RDIVNIDISE VVIKQMKECN ATRRPQMSFL KMDMTQMEFP DASFQVVLDK 120
GTLDAVLTDE EEKTLQQVDR MLAEVGRVLQ VGGRYLCISL AQAHILKKAV GHFSREGWMV 180
RVHQVANSQD QVLEAEPQFS LPVFAFIMTK FRPVPGSALQ IFELCAQEQR KPVRLESAER 240
LAEAVQERQQ YAWLCSQLRR KARLGSVSLD LCDGDTGEPR YTLHVVDSPT VKPSRDNHFA 300
IFIIPQGRET EWLFGMDEGR KQLAASAGFR RLITVALHRG QQYESMDHIQ AELSARVMEL 360
APAGMPTQQQ VPFLSVGGDI GVRTVQHQDC SPLSGDYVIE DVQGDDKRYF RRLIFLSNRN 420
VVQSEARLLK DVSHKAQKKR KKDRKKQRPA DAEDLPAAPG QSIDKSYLCC EHHKAMIAGL 480
ALLRNPELLL EIPLALLVVG LGGGSLPLFV HDHFPKSCID AVEIDPSMLE VATQWFGFSQ 540
SDRMKVHIAD GLDYIASLAG GGEARPCYDV IMFDVDSKDP TLGMSCPPPA FVEQSFLQKV 600
KSILTPEGVF ILNLVCRDLG LKDSVLAGLK AVFPLLYVRR IEGEVNEILF CQLHPEQKLA 660
TPELLETAQA LERTLRKPGR GWDDTYVLSD MLKTVKIV 698 
Gene Ontology
 GO:0008168; F:methyltransferase activity; IEA:InterPro.
 GO:0032259; P:methylation; IEA:GOC. 
Interpro
 IPR013216; Methyltransf_11. 
Pfam
 PF08241; Methyltransf_11 
SMART
  
PROSITE
  
PRINTS