CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-019153
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 RNA-binding protein Musashi homolog 2 
Protein Synonyms/Alias
 Musashi-2 
Gene Name
 MSI2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
43SLRDYFSKFGEIRECubiquitination[1, 2, 3, 4]
59VMRDPTTKRSRGFGFubiquitination[4]
77ADPASVDKVLGQPHHubiquitination[4]
89PHHELDSKTIDPKVAubiquitination[4]
94DSKTIDPKVAFPRRAubiquitination[4]
104FPRRAQPKMVTRTKKubiquitination[4]
135QYFEQFGKVEDAMLMubiquitination[2]
145DAMLMFDKTTNRHRGubiquitination[1, 2, 3, 4]
166ENEDVVEKVCEIHFHubiquitination[4]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 RNA binding protein that regulates the expression of target mRNAs at the translation level. May play a role in the proliferation and maintenance of stem cells in the central nervous system (By similarity). 
Sequence Annotation
 DOMAIN 21 111 RRM 1.
 DOMAIN 110 187 RRM 2.
 MOD_RES 1 1 N-acetylmethionine.  
Keyword
 Acetylation; Alternative splicing; Chromosomal rearrangement; Complete proteome; Cytoplasm; Phosphoprotein; Reference proteome; Repeat; RNA-binding. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 328 AA 
Protein Sequence
MEANGSQGTS GSANDSQHDP GKMFIGGLSW QTSPDSLRDY FSKFGEIREC MVMRDPTTKR 60
SRGFGFVTFA DPASVDKVLG QPHHELDSKT IDPKVAFPRR AQPKMVTRTK KIFVGGLSAN 120
TVVEDVKQYF EQFGKVEDAM LMFDKTTNRH RGFGFVTFEN EDVVEKVCEI HFHEINNKMV 180
ECKKAQPKEV MFPPGTRGRA RGLPYTMDAF MLGMGMLGYP NFVATYGRGY PGFAPSYGYQ 240
FPGFPAAAYG PVAAAAVAAA RGSGSNPARP GGFPGANSPG PVADLYGPAS QDSGVGNYIS 300
AASPQPGSGF GHGIAGPLIA TAFTNGYH 328 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005844; C:polysome; IEA:Compara.
 GO:0000166; F:nucleotide binding; IEA:InterPro.
 GO:0008266; F:poly(U) RNA binding; IEA:Compara.
 GO:0048864; P:stem cell development; IEA:Compara. 
Interpro
 IPR012677; Nucleotide-bd_a/b_plait.
 IPR000504; RRM_dom. 
Pfam
 PF00076; RRM_1 
SMART
 SM00360; RRM 
PROSITE
 PS50102; RRM 
PRINTS