CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012256
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Unhealthy ribosome biogenesis protein 2 homolog 
Protein Synonyms/Alias
  
Gene Name
 URB2 
Gene Synonyms/Alias
 KIAA0133 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
57QSLVAFYKKKLELKEubiquitination[1, 2]
59LVAFYKKKLELKEDIacetylation[3]
255PELLSSYKEGLLDQQubiquitination[1, 2, 4, 5, 6, 7, 8]
267DQQQGDVKTGAMKNLubiquitination[1, 2, 6, 7]
272DVKTGAMKNLLAPMDubiquitination[1, 2, 6, 7, 8]
336PRLFGCLKISHLQEEubiquitination[2, 6]
447VTEFRTKKAQEALIRubiquitination[6, 8]
462TVFQTYAKLRQVPRLubiquitination[2, 6, 9, 10]
844QLPWLFEKDHMVVGHubiquitination[1, 2]
858HWENRFAKAGPEGIEubiquitination[1, 2, 4, 5, 6, 7, 8]
1034TAFLSSSKPYTEAASubiquitination[1]
1043YTEAASSKQLENQNPubiquitination[1, 2]
1072HVLGPFLKEQKLGQEacetylation[8]
1072HVLGPFLKEQKLGQEubiquitination[2, 6]
1195APELHPKKDSVFTSMubiquitination[2, 6]
1286NCPLSGEKASLLWRAubiquitination[2, 6]
1387CHPKVMLKAIPSFLNubiquitination[1, 2, 4, 6, 9, 10]
1412REGRQKDKGSIDDLPubiquitination[1, 6]
1423DDLPTVLKCARLVERubiquitination[2, 6, 8]
1502PGMRDIFKELYNDYLubiquitination[1, 6]
1510ELYNDYLKYHKAKHEubiquitination[6]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [3] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786]
 [4] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [6] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [7] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [8] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [9] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [10] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Nucleus; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1524 AA 
Protein Sequence
MAAVYSGISL KLKSKTTSWE DKLKLAHFAW ISHQCFLPNK EQVLLDWARQ SLVAFYKKKL 60
ELKEDIVERL WIYIDNILHS RKLQNLLKNG KTINLQISLV KIINERVAEF SLSGSQRNIC 120
AVLRCCQGIL STPALAVIYT AKQELMVALL SQLCWSACRQ PEGAVVAQLF EVIHLALGHY 180
LLILQQQVNP RRAFGDVTAH LLQPCLVLRH LLSGGTWTQA GQGQLRQVLS RDIRSQIEAM 240
FRGGIFQPEL LSSYKEGLLD QQQGDVKTGA MKNLLAPMDT VLNRLVDAGY CAASLHTSVV 300
ANSVALLYKL FLDSYFKEGN QLLCFQVLPR LFGCLKISHL QEEQSKALST SDWTTELLVV 360
EQLLNSVANN NIYNIAADRI RHEEAQFRFY RHVAELLINH AQAPIPAWFR CLKTLISLNH 420
LILEPDLDDL LASAWIDAEV TEFRTKKAQE ALIRTVFQTY AKLRQVPRLF EEVLGVICRP 480
AAEALRQPVL ASGPSTVLSA CLLELPPSQI LDTWSLVLEK FQSLVLPYLQ SDADMALKSL 540
SLSLLLHCIM FNMRSLDSST PLPIVRRTQC MMERMMRELV QPLLALLPDT PGPEPELWLQ 600
KVSDSVLLLS YTWAQVDAMF SLNCSQYHSM SGPLIGVALE ISNLPSLLPG VKTQHWKKIE 660
KFTAQFSSLG TYCLEQLYLQ KMKRTLMQTS FRSEGAIQSL RCDAAFIIGS GRKSLNQRTT 720
ASWDGQVGMV SGLTYPVAHW HLIVSNLTIL ISYLCPDDVG YLASVLLRTL PMGKAQEVSI 780
DEEAYITLEK ISKAFLHSPL FPEMQSLHSA FLTCVTTSCS SILCSGAQRD SGLVSQQLPW 840
LFEKDHMVVG HWENRFAKAG PEGIEPRGEI AQNLLSLVKS DFPIQLEGEQ LESILGLLEV 900
ISALQLDSLL PPYHVHYFLV LLSMAVTKLG CSCSSSLALK FLTTCYQLLG YLQKGKSARS 960
VFKIMYGSDI FEVVLTSLFR ASSRFLIEMD DPAWLEFLQV IGTFLEELMQ MLIQMKLSLV 1020
LNFRKITAFL SSSKPYTEAA SSKQLENQNP QGRQLLLVSL TRLCHVLGPF LKEQKLGQEA 1080
PAALSELLQQ VVLQTGAVLQ LCSVPGARGW RLPSVLISSV STLLEADLGQ HCRDGGADIS 1140
QGSDRTLLSH VALYQGVYSQ ILLELPALAG HDQSFQAALQ FLTLFFLAPE LHPKKDSVFT 1200
SMFHSVRRVL ADPEIPVQVT QDIEPHLGAL FTQMLEVGTT EDLRLVMQCI LQGLDVSNMW 1260
KADVQAVVSA VTLLRLLLNC PLSGEKASLL WRACPQIVTA LTLLNREASQ EQPVSLTVVG 1320
PVLDVLAALL RQGEEAIGNP HHVSLAFSIL LTVPLDHLKP LEYGSVFPRL HNVLFSILQC 1380
HPKVMLKAIP SFLNSFNRLV FSVMREGRQK DKGSIDDLPT VLKCARLVER MYSHIAARAE 1440
EFAVFSPFMV AQYVLEVQKV TLYPAVKSLL QEGIYLILDL CIEPDVQFLR ASLQPGMRDI 1500
FKELYNDYLK YHKAKHEGEK RYTA 1524 
Gene Ontology
 GO:0005730; C:nucleolus; IDA:HPA. 
Interpro
 IPR018849; Urb2/Npa2_C. 
Pfam
 PF10441; Urb2 
SMART
  
PROSITE
  
PRINTS