UniProt Accession

 NSP1_YEAST; P14907; D6VWE3 

Genbank Protein ID

 M37160; Z49316; Z49314; BK006943 

Genbank Nucleotide ID

 AAA34821.1; CAA89332.1; CAA89331.1; DAA08759.1 

Protein Name

 Nucleoporin NSP1 

Protein Synonyms/Alias

 Nuclear pore protein NSP1; Nucleoskeletal-like protein; p110 

Gene Name

 NSP1 

Gene Synonyms/Alias

 YJL041W; J1207 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
308	PAFSFGAKPEEKKDD	acetylation	[1]
613	GKSTADVKSSDSLKL	ubiquitination	[2]
623	DSLKLNSKPVELKPV	ubiquitination	[2]
795	DENIQLIKILNSHFD	ubiquitination	[2]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 

Functional Description

 Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. Active directional transport is assured by both, a Phe-Gly (FG) repeat affinity gradient for these transport factors across the NPC and a transport cofactor concentration gradient across the nuclear envelope (GSP1 and GSP2 GTPases associated predominantly with GTP in the nucleus, with GDP in the cytoplasm). NSP1 plays an important role in several nuclear transport pathways including poly(A)+ RNA, tRNA, pre-ribosome, signal recognition particle (SRP), and protein transport. 

Sequence Annotation

 REPEAT 13 16 FSFG 1.
 REPEAT 36 37 FG 1.
 REPEAT 43 44 FG 2.
 REPEAT 64 65 FG 3.
 REPEAT 74 75 FG 4.
 REPEAT 84 85 FG 5.
 REPEAT 94 95 FG 6.
 REPEAT 104 105 FG 7.
 REPEAT 123 124 FG 8.
 REPEAT 142 143 FG 9.
 REPEAT 147 148 FG 10.
 REPEAT 168 169 FG 11.
 REPEAT 179 182 FSFG 2.
 REPEAT 217 220 FSFG 3.
 REPEAT 239 240 FG 12.
 REPEAT 268 269 FG 13.
 REPEAT 284 287 FSFG 4.
 REPEAT 303 306 FSFG 5.
 REPEAT 322 325 FSFG 6.
 REPEAT 341 344 FSFG 7.
 REPEAT 360 363 FSFG 8.
 REPEAT 379 382 FSFG 9.
 REPEAT 398 401 FSFG 10.
 REPEAT 417 420 FSFG 11.
 REPEAT 436 439 FSFG 12.
 REPEAT 455 458 FSFG 13.
 REPEAT 474 477 FSFG 14.
 REPEAT 493 496 FSFG 15.
 REPEAT 512 515 FSFG 16.
 REPEAT 531 534 FSFG 17.
 REPEAT 550 553 FSFG 18.
 REPEAT 571 572 FG 14.
 REPEAT 588 591 FSFG 19; approximate.
 REGION 673 738 Competitive binding site of NUP57 and
 REGION 740 823 Required for association with NUP57
 MOD_RES 238 238 Phosphoserine.
 MOD_RES 361 361 Phosphoserine.
 MOD_RES 456 456 Phosphoserine.
 MOD_RES 631 631 Phosphoserine.  

Keyword

 3D-structure; Cell cycle; Coiled coil; Complete proteome; Membrane; mRNA transport; Nuclear pore complex; Nucleus; Phosphoprotein; Protein transport; Reference proteome; Repeat; Translocation; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 823 AA 

Protein Sequence

Gene Ontology

 GO:0031965; C:nuclear membrane; IEA:UniProtKB-SubCell.
 GO:0034399; C:nuclear periphery; IDA:SGD.
 GO:0044613; C:nuclear pore central transport channel; IDA:SGD.
 GO:0044615; C:nuclear pore nuclear basket; IDA:SGD.
 GO:0005487; F:nucleocytoplasmic transporter activity; IPI:SGD.
 GO:0005543; F:phospholipid binding; IDA:SGD.
 GO:0017056; F:structural constituent of nuclear pore; IDA:SGD.
 GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
 GO:0097064; P:ncRNA export from nucleus; IMP:SGD.
 GO:0006607; P:NLS-bearing substrate import into nucleus; IGI:SGD.
 GO:0016973; P:poly(A)+ mRNA export from nucleus; IGI:SGD.
 GO:0000055; P:ribosomal large subunit export from nucleus; IMP:SGD.
 GO:0000056; P:ribosomal small subunit export from nucleus; IMP:SGD.
 GO:0006409; P:tRNA export from nucleus; IMP:SGD. 

Interpro

 IPR026010; NSP1/NUP62.
 IPR007758; Nucleoporin_NSP1_C. 

Pfam

 PF05064; Nsp1_C 

SMART

  

PROSITE

  

PRINTS