UniProt Accession

 B4DY72_HUMAN; B4DY72 

Genbank Protein ID

 AL137142; AK302294 

Genbank Nucleotide ID

 BAG63634.1 

Protein Name

 Heat shock protein 105 kDa 

Protein Synonyms/Alias

 cDNA FLJ52360, highly similar to Heat-shock protein 105 kDa 

Gene Name

 HSPH1 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
53	FDPFLGGKNFDEKLV	ubiquitination	[1, 2, 3]
58	GGKNFDEKLVEHFCA	ubiquitination	[1, 3]
68	EHFCAEFKTKYKLDA	ubiquitination	[1]
91	RLYQECEKLKKLMSS	acetylation	[3, 4, 5]
91	RLYQECEKLKKLMSS	ubiquitination	[1]
93	YQECEKLKKLMSSNS	ubiquitination	[1]
94	QECEKLKKLMSSNST	ubiquitination	[1]
114	IECFMNDKDVSGKMN	ubiquitination	[1]
119	NDKDVSGKMNRSQFE	ubiquitination	[1]
170	ATRIPAVKERIAKFF	ubiquitination	[1, 3]
175	AVKERIAKFFGKDIS	ubiquitination	[1]
179	RIAKFFGKDISTTLN	ubiquitination	[1, 6]
207	AILSPAFKVREFSVT	ubiquitination	[1, 3, 6, 7, 8, 9, 10]
249	NHAAPFSKVLTFLRR	acetylation	[4]
249	NHAAPFSKVLTFLRR	ubiquitination	[1, 7, 8, 9, 10]
277	GVPYPEAKIGRFVVQ	ubiquitination	[1, 2, 3, 6, 8, 9, 10]
290	VQNVSAQKDGEKSRV	acetylation	[5]
290	VQNVSAQKDGEKSRV	ubiquitination	[1, 2, 3, 8]
445	GKMIMQDKLEKERND	acetylation	[5]
445	GKMIMQDKLEKERND	ubiquitination	[1, 8]
467	YVYEFRDKLCGPYEK	ubiquitination	[1]
474	KLCGPYEKFICEQDH	ubiquitination	[1, 9, 10]
510	AKQAYVDKLEELMKI	acetylation	[5]
510	AKQAYVDKLEELMKI	ubiquitination	[7]
516	DKLEELMKIGTPVKV	ubiquitination	[1]
522	MKIGTPVKVRFQEAE	ubiquitination	[1, 2]
568	HIDESEMKKVEKSVN	ubiquitination	[1]
572	SEMKKVEKSVNEVME	ubiquitination	[1]
609	QEIKTKIKELNNTCE	ubiquitination	[3, 7]
630	KPKIESPKLERTPNG	acetylation	[3]
642	PNGPNIDKKEEDLED	acetylation	[5]
669	GECYPNEKNSVNMDL	ubiquitination	[9]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
 [5] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [6] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [7] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [8] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [9] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [10] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931] 

Functional Description

  

Sequence Annotation

  

Keyword

 ATP-binding; Complete proteome; Nucleotide-binding; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 677 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW. 

Interpro

 IPR018181; Heat_shock_70_CS.
 IPR013126; Hsp_70_fam. 

Pfam

 PF00012; HSP70 

SMART

  

PROSITE

 PS01036; HSP70_3 

PRINTS

 PR00301; HEATSHOCK70.