CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-011856
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 3-keto-steroid reductase 
Protein Synonyms/Alias
  
Gene Name
 ERG27 
Gene Synonyms/Alias
 YLR100W 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
134AVTNPTYKIQLVGVKubiquitination[1]
291LANPNFEKQDVKYGSubiquitination[1]
295NFEKQDVKYGSATSRubiquitination[1]
309RDGMPYIKTQEIDPTubiquitination[1]
337LEWDEKLKDQIVETRubiquitination[1]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 Responsible for the reduction of the keto group on the C-3 of sterols. Also facilitates the association of ERG7 with lipid particles preventing its digestion in the endoplasmic reticulum and the lipid particles. 
Sequence Annotation
 ACT_SITE 202 202 Proton acceptor (By similarity).
 MOD_RES 345 345 Phosphothreonine.  
Keyword
 Complete proteome; Endoplasmic reticulum; Lipid biosynthesis; Lipid droplet; Lipid metabolism; Membrane; NADP; Oxidoreductase; Phosphoprotein; Reference proteome; Steroid biosynthesis. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 347 AA 
Protein Sequence
MNRKVAIVTG TNSNLGLNIV FRLIETEDTN VRLTIVVTSR TLPRVQEVIN QIKDFYNKSG 60
RVEDLEIDFD YLLVDFTNMV SVLNAYYDIN KKYRAINYLF VNAAQGIFDG IDWIGAVKEV 120
FTNPLEAVTN PTYKIQLVGV KSKDDMGLIF QANVFGPYYF ISKILPQLTR GKAYIVWISS 180
IMSDPKYLSL NDIELLKTNA SYEGSKRLVD LLHLATYKDL KKLGINQYVV QPGIFTSHSF 240
SEYLNFFTYF GMLCLFYLAR LLGSPWHNID GYKAANAPVY VTRLANPNFE KQDVKYGSAT 300
SRDGMPYIKT QEIDPTGMSD VFAYIQKKKL EWDEKLKDQI VETRTPI 347 
Gene Ontology
 GO:0005789; C:endoplasmic reticulum membrane; IDA:SGD.
 GO:0005811; C:lipid particle; IEA:UniProtKB-SubCell.
 GO:0005741; C:mitochondrial outer membrane; IDA:SGD.
 GO:0000253; F:3-keto sterol reductase activity; IMP:SGD.
 GO:0006696; P:ergosterol biosynthetic process; IMP:SGD. 
Interpro
 IPR016040; NAD(P)-bd_dom. 
Pfam
  
SMART
  
PROSITE
  
PRINTS