CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-029069
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 40S ribosomal protein SA 
Protein Synonyms/Alias
 37 kDa laminin receptor precursor; 37/67 kDa laminin receptor; 67 kDa laminin receptor; Laminin receptor 1; Laminin-binding protein precursor p40 
Gene Name
 RPSAP58 
Gene Synonyms/Alias
 RPSA 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
11ALDVLQMKEEDVLKFubiquitination[1, 2, 3]
42EHYIYKRKSDGIYIIubiquitination[2, 3]
52GIYIINLKRTWEKLLubiquitination[2, 3, 4, 5]
57NLKRTWEKLLLAARAubiquitination[2, 3, 4, 5, 6]
89TGQRAVLKFAAATGAubiquitination[1, 2, 3, 4, 5, 6, 7, 8]
220EEQAAAEKAVTKEEFacetylation[9]
220EEQAAAEKAVTKEEFubiquitination[5, 7]
Reference
 [1] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [6] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [7] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [8] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [9] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377] 
Functional Description
 Required for the assembly and/or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA- precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. Also functions as a cell surface receptor for laminin. Plays a role in cell adhesion to the basement membrane and in the consequent activation of signaling transduction pathways. May play a role in cell fate determination and tissue morphogenesis. Also acts as a receptor for several other ligands, including the pathogenic prion protein, viruses, and bacteria. Acts as a PPP1R16B-dependent substrate of PPP1CA (By similarity). 
Sequence Annotation
 REPEAT 230 232 -W-[ST] 1 (By similarity).
 REPEAT 247 249 -W-[ST] 2 (By similarity).
 REPEAT 266 268 -W-[ST] 3 (By similarity).
 REPEAT 275 277 -W-[ST] 4 (By similarity).
 REPEAT 293 295 -W-[ST] 5 (By similarity).
 REGION 54 113 Interaction with PPP1R16B (By
 REGION 161 180 Laminin-binding (By similarity).
 REGION 205 229 Laminin-binding (By similarity).
 REGION 242 295 Laminin-binding (By similarity).
 MOD_RES 2 2 N-acetylserine (By similarity).  
Keyword
 Acetylation; Cell membrane; Complete proteome; Cytoplasm; Membrane; Nucleus; Receptor; Reference proteome; Repeat; Ribonucleoprotein; Ribosomal protein. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 295 AA 
Protein Sequence
MSGALDVLQM KEEDVLKFLA AGTHLGGTNL DFQMEHYIYK RKSDGIYIIN LKRTWEKLLL 60
AARAIVAIEN PADVSVISSR NTGQRAVLKF AAATGATPIA GRFTPGTFTN QIQAAFWEPR 120
LLVVTDPRAD HQPLTEASYV NLPTIALCNT DSPLRYVDIA IPCNNKGAHS VGLMWWMLAR 180
EVLRMRGTIS REHPWEVMPD LYFYRDPEEI EKEEQAAAEK AVTKEEFQGE WTAPSPEFTA 240
TQPEVADWSE GVQVPSVPIQ QFPTEDWSAQ PATEDWSAAP TAQATEWVGA TTDWS 295 
Gene Ontology
 GO:0022627; C:cytosolic small ribosomal subunit; IEA:HAMAP.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
 GO:0005055; F:laminin receptor activity; IEA:HAMAP.
 GO:0003735; F:structural constituent of ribosome; IEA:HAMAP.
 GO:0007155; P:cell adhesion; IEA:HAMAP.
 GO:0000028; P:ribosomal small subunit assembly; IEA:HAMAP.
 GO:0006412; P:translation; IEA:HAMAP. 
Interpro
 IPR027504; 40S_ribosomal_SA.
 IPR001865; Ribosomal_S2.
 IPR018130; Ribosomal_S2_CS.
 IPR027498; Ribosomal_S2_euk.
 IPR005707; Ribosomal_S2_euk/arc.
 IPR023591; Ribosomal_S2_flav_dom. 
Pfam
 PF00318; Ribosomal_S2 
SMART
  
PROSITE
 PS00962; RIBOSOMAL_S2_1
 PS00963; RIBOSOMAL_S2_2 
PRINTS
 PR00395; RIBOSOMALS2.