UniProt Accession

 RHBT3_HUMAN; O94955; A0PJA4; A8K1W9; Q8IW06 

Genbank Protein ID

 AB020685; AK290034; BC020231; BC041337 

Genbank Nucleotide ID

 BAA74901.2; BAF82723.1; AAH20231.1; AAH41337.1 

Protein Name

 Rho-related BTB domain-containing protein 3 

Protein Synonyms/Alias

  

Gene Name

 RHOBTB3 

Gene Synonyms/Alias

 KIAA0878 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
105	IKYNVNDKFSFHEVK	ubiquitination	[1]
120	DNYIPVIKRALNSVP	ubiquitination	[1, 2, 3, 4, 5]
211	SEKMKKRKMSNSFHG	ubiquitination	[3]
332	PPLRVIVKDALFCSC	ubiquitination	[6]
366	EDIRKKLKDSGDVSN	ubiquitination	[2, 4, 5, 6]
377	DVSNVIEKVKCILKT	ubiquitination	[2, 5]
387	CILKTPGKINCLRNC	ubiquitination	[4]
401	CKTYQARKPLWFYNT	ubiquitination	[3]
538	LDIVDLLKKAKFHHS	ubiquitination	[1, 2, 5]
592	WPSNMYLKQLAEYRK	ubiquitination	[1, 3, 5]

Reference

 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [6] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572] 

Functional Description

 Rab9-regulated ATPase required for endosome to Golgi transport. Involved in transport vesicle docking at the Golgi complex, possibly by participating in release M6PRBP1/TIP47 from vesicles to permit their efficient docking and fusion at the Golgi. Specifically binds Rab9, but not other Rab proteins. Has low intrinsic ATPase activity due to autoinhibition, which is relieved by Rab9. 

Sequence Annotation

 DOMAIN 254 356 BTB 1.
 DOMAIN 420 487 BTB 2.
 REGION 1 175 Rho-like.
 REGION 420 611 Interaction with Rab9.  

Keyword

 ATP-binding; Complete proteome; Golgi apparatus; Hydrolase; Nucleotide-binding; Polymorphism; Reference proteome; Repeat; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 611 AA 

Protein Sequence

Gene Ontology

 GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IDA:UniProtKB.
 GO:0016887; F:ATPase activity; IDA:UniProtKB.
 GO:0005525; F:GTP binding; IEA:InterPro.
 GO:0042147; P:retrograde transport, endosome to Golgi; IMP:UniProtKB.
 GO:0007264; P:small GTPase mediated signal transduction; IEA:InterPro. 

Interpro

 IPR000210; BTB/POZ-like.
 IPR011333; BTB/POZ_fold.
 IPR013069; BTB_POZ.
 IPR027417; P-loop_NTPase.
 IPR001806; Small_GTPase. 

Pfam

 PF00651; BTB
 PF00071; Ras 

SMART

 SM00225; BTB 

PROSITE

 PS50097; BTB 

PRINTS