UniProt Accession

 PEX19_HUMAN; P40855; D3DVE7; Q5QNY4; Q8NI97 

Genbank Protein ID

 X75535; Y09048; AB018541; AB062286; BT006879; AL513282; CH471121; CH471121; BC000496 

Genbank Nucleotide ID

 CAA53225.1; CAA70257.1; BAA76291.1; BAB93469.1; AAP35525.1; CAI12457.1; EAW52728.1; EAW52729.1; AAH00496.1 

Protein Name

 Peroxisomal biogenesis factor 19 

Protein Synonyms/Alias

 33 kDa housekeeping protein; Peroxin-19; Peroxisomal farnesylated protein 

Gene Name

 PEX19 

Gene Synonyms/Alias

 HK33; PXF; OK/SW-cl.22 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
33	LDDFDKAKPSPAPPS	ubiquitination	[1]
52	PDASGPQKRSPGDTA	ubiquitination	[1, 2, 3, 4]
60	RSPGDTAKDALFASQ	ubiquitination	[1, 2, 3, 4, 5, 6, 7, 8]
89	QATAEFEKAMKELAE	ubiquitination	[4]
92	AEFEKAMKELAEEEP	ubiquitination	[3]
107	HLVEQFQKLSEAAGR	ubiquitination	[1, 3, 4, 5, 8]
130	QEFTSCLKETLSGLA	ubiquitination	[1, 3, 5, 7, 8, 9]
138	ETLSGLAKNATDLQN	ubiquitination	[1, 3, 4]
245	TDSETTQKARFEMVL	ubiquitination	[1, 3, 4, 6]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [6] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [7] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [8] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [9] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572] 

Functional Description

 Necessary for early peroxisomal biogenesis. Acts both as a cytosolic chaperone and as an import receptor for peroxisomal membrane proteins (PMPs). Binds and stabilizes newly synthesized PMPs in the cytoplasm by interacting with their hydrophobic membrane-spanning domains, and targets them to the peroxisome membrane by binding to the integral membrane protein PEX3. Excludes CDKN2A from the nucleus and prevents its interaction with MDM2, which results in active degradation of TP53. 

Sequence Annotation

 REGION 1 91 Necessary for PEX19 function on
 REGION 1 56 Docking to the peroxisome membrane and
 MOD_RES 296 296 Cysteine methyl ester (Probable).
 LIPID 296 296 S-farnesyl cysteine.  

Keyword

 3D-structure; Alternative splicing; Complete proteome; Cytoplasm; Lipoprotein; Membrane; Methylation; Peroxisome; Peroxisome biogenesis; Peroxisome biogenesis disorder; Prenylation; Reference proteome; Zellweger syndrome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 299 AA 

Protein Sequence

Gene Ontology

 GO:0031526; C:brush border membrane; ISS:UniProtKB.
 GO:0005829; C:cytosol; IDA:UniProtKB.
 GO:0016021; C:integral to membrane; IDA:UniProtKB.
 GO:0005634; C:nucleus; IMP:UniProtKB.
 GO:0005778; C:peroxisomal membrane; IDA:UniProtKB.
 GO:0043234; C:protein complex; IDA:UniProtKB.
 GO:0036105; F:peroxisome membrane class-1 targeting sequence binding; IDA:UniProtKB.
 GO:0061077; P:chaperone-mediated protein folding; IDA:UniProtKB.
 GO:0072321; P:chaperone-mediated protein transport; IDA:UniProtKB.
 GO:1900131; P:negative regulation of lipid binding; IDA:UniProtKB.
 GO:0016559; P:peroxisome fission; IMP:UniProtKB.
 GO:0016557; P:peroxisome membrane biogenesis; IDA:UniProtKB.
 GO:0007031; P:peroxisome organization; IMP:UniProtKB.
 GO:0045046; P:protein import into peroxisome membrane; IDA:UniProtKB.
 GO:0050821; P:protein stabilization; IDA:UniProtKB. 

Interpro

 IPR006708; Pex19. 

Pfam

 PF04614; Pex19 

SMART

  

PROSITE

  

PRINTS