CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-031077
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 HCG2005638, isoform CRA_c 
Protein Synonyms/Alias
 Spliceosome RNA helicase DDX39B; cDNA FLJ53073, highly similar to Spliceosome RNA helicase Bat1 (EC 3.6.1.-) 
Gene Name
 DDX39B 
Gene Synonyms/Alias
 hCG_2005638 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
163EIRPVCRKFMQDPMEubiquitination[1, 2]
190GLQQYYVKLKDNEKNubiquitination[1, 2, 3]
192QQYYVKLKDNEKNRKubiquitination[3]
256LSRYQQFKDFQRRILacetylation[4]
256LSRYQQFKDFQRRILubiquitination[1, 3, 5, 6, 7]
306RAGRFGTKGLAITFVubiquitination[2, 3, 6, 7, 8]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [5] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [6] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [7] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [8] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661
Functional Description
  
Sequence Annotation
  
Keyword
 ATP-binding; Complete proteome; Helicase; Hydrolase; Nucleotide-binding; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 350 AA 
Protein Sequence
MWTMSSWTMK MMRWRQQLGE MGLRPLPRRM SRAPMSPSTA LAFVTSCSSQ SCSGPLSTVA 60
LSIRQKVAVF FGGLSIKKDE EVLKKNCPHI VVGTPGRILA LARNKSLNLK HIKHFILDEC 120
DKMLEQLDMR RDVQEIFRMT PHEKQVMMFS ATLSKEIRPV CRKFMQDPME IFVDDETKLT 180
LHGLQQYYVK LKDNEKNRKL FDLLDVLEFN QVVIFVKSVQ RCIALAQLLV EQNFPAIAIH 240
RGMPQEERLS RYQQFKDFQR RILVATNLFG RGMDIERVNI AFNYDMPEDS DTYLHRVARA 300
GRFGTKGLAI TFVSDENDAK ILNDVQDRFE VNISELPDEI DISSYIEQTR 350 
Gene Ontology
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0008026; F:ATP-dependent helicase activity; IEA:InterPro.
 GO:0003676; F:nucleic acid binding; IEA:InterPro. 
Interpro
 IPR011545; DNA/RNA_helicase_DEAD/DEAH_N.
 IPR014001; Helicase_ATP-bd.
 IPR001650; Helicase_C.
 IPR027417; P-loop_NTPase. 
Pfam
 PF00270; DEAD
 PF00271; Helicase_C 
SMART
 SM00487; DEXDc
 SM00490; HELICc 
PROSITE
 PS51192; HELICASE_ATP_BIND_1
 PS51194; HELICASE_CTER 
PRINTS