UniProt Accession

 GRCA_ECOLI; P68066; P33633 

Genbank Protein ID

 D13169; D64044; U00096; AP009048; J03725 

Genbank Nucleotide ID

 AAC75632.1; BAA16465.1 

Protein Name

 Autonomous glycyl radical cofactor 

Protein Synonyms/Alias

  

Gene Name

 grcA 

Gene Synonyms/Alias

 yfiD; b2579; JW2563 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
26	FWLLDSEKGEARCIV	acetylation	[1]
48	DEVVAVSKLGDIEYR	acetylation	[1, 2]
62	REVPVEVKPEVRVEG	acetylation	[1, 3]
88	ETLEDAVKHPEKYPQ	acetylation	[1, 2]
92	DAVKHPEKYPQLTIR	acetylation	[1, 2]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618]
 [2] Lysine acetylation is a highly abundant and evolutionarily conserved modification in Escherichia coli.
 Zhang J, Sprung R, Pei J, Tan X, Kim S, Zhu H, Liu CF, Grishin NV, Zhao Y.
 Mol Cell Proteomics. 2009 Feb;8(2):215-25. [PMID: 18723842]
 [3] Comprehensive profiling of protein lysine acetylation in Escherichia coli.
 Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z.
 J Proteome Res. 2013 Feb 1;12(2):844-51. [PMID: 23294111] 

Functional Description

 Acts as a radical domain for damaged PFL and possibly other radical proteins. 

Sequence Annotation

 DOMAIN 5 127 Glycine radical.
 MOD_RES 48 48 N6-acetyllysine.
 MOD_RES 88 88 N6-acetyllysine.
 MOD_RES 92 92 N6-acetyllysine.
 MOD_RES 102 102 Glycine radical (By similarity).  

Keyword

 Acetylation; Complete proteome; Direct protein sequencing; Organic radical; Reference proteome; Stress response. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 127 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:UniProtKB.
 GO:0003824; F:catalytic activity; IEA:InterPro.
 GO:0006950; P:response to stress; IEA:UniProtKB-KW. 

Interpro

 IPR019777; Form_AcTrfase_GR_CS.
 IPR001150; Gly_radical.
 IPR011140; Glycyl_radical_cofactor_GrcA. 

Pfam

 PF01228; Gly_radical 

SMART

  

PROSITE

 PS00850; GLY_RADICAL_1
 PS51149; GLY_RADICAL_2 

PRINTS