CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004929
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Fatty acid oxidation complex subunit alpha 
Protein Synonyms/Alias
 Enoyl-CoA hydratase/Delta(3)-cis-Delta(2)-trans-enoyl-CoA isomerase/3-hydroxybutyryl-CoA epimerase; 3-hydroxyacyl-CoA dehydrogenase 
Gene Name
 fadB 
Gene Synonyms/Alias
 oldB; b3846; JW3822 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
141RIGLPETKLGIMPGFacetylation[1]
171LEIIAAGKDVGADQAacetylation[1]
180VGADQALKIGLVDGVacetylation[1]
189GLVDGVVKAEKLVEGacetylation[1]
256PAPITAVKTIEAAARacetylation[1]
347VMKDINDKSLTLGMTacetylation[1]
358LGMTEAAKLLNKQLEacetylation[1]
368NKQLERGKIDGLKLAacetylation[1]
373RGKIDGLKLAGVISTacetylation[1]
525ADFRKIDKVMEKQFGacetylation[1]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 
Functional Description
 Catalyzes the formation of a hydroxyacyl-CoA by addition of water on enoyl-CoA. Also exhibits 3-hydroxyacyl-CoA epimerase and 3-hydroxyacyl-CoA dehydrogenase activities. Involved in the aerobic and anaerobic degradation of long-chain fatty acids. 
Sequence Annotation
 NP_BIND 400 402 NAD (By similarity).
 NP_BIND 427 429 NAD (By similarity).
 REGION 1 189 Enoyl-CoA hydratase/isomerase.
 REGION 311 729 3-hydroxyacyl-CoA dehydrogenase.
 ACT_SITE 450 450 For 3-hydroxyacyl-CoA dehydrogenase
 BINDING 296 296 Substrate (By similarity).
 BINDING 324 324 NAD; via amide nitrogen (Probable).
 BINDING 343 343 NAD (By similarity).
 BINDING 407 407 NAD (By similarity).
 BINDING 453 453 NAD (By similarity).
 BINDING 500 500 Substrate (By similarity).
 BINDING 660 660 Substrate (By similarity).  
Keyword
 Complete proteome; Direct protein sequencing; Fatty acid metabolism; Isomerase; Lipid degradation; Lipid metabolism; Lyase; Multifunctional enzyme; NAD; Oxidoreductase; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 729 AA 
Protein Sequence
MLYKGDTLYL DWLEDGIAEL VFDAPGSVNK LDTATVASLG EAIGVLEQQS DLKGLLLRSN 60
KAAFIVGADI TEFLSLFLVP EEQLSQWLHF ANSVFNRLED LPVPTIAAVN GYALGGGCEC 120
VLATDYRLAT PDLRIGLPET KLGIMPGFGG SVRMPRMLGA DSALEIIAAG KDVGADQALK 180
IGLVDGVVKA EKLVEGAKAV LRQAINGDLD WKAKRQPKLE PLKLSKIEAT MSFTIAKGMV 240
AQTAGKHYPA PITAVKTIEA AARFGREEAL NLENKSFVPL AHTNEARALV GIFLNDQYVK 300
GKAKKLTKDV ETPKQAAVLG AGIMGGGIAY QSAWKGVPVV MKDINDKSLT LGMTEAAKLL 360
NKQLERGKID GLKLAGVIST IHPTLDYAGF DRVDIVVEAV VENPKVKKAV LAETEQKVRQ 420
DTVLASNTST IPISELANAL ERPENFCGMH FFNPVHRMPL VEIIRGEKSS DETIAKVVAW 480
ASKMGKTPIV VNDCPGFFVN RVLFPYFAGF SQLLRDGADF RKIDKVMEKQ FGWPMGPAYL 540
LDVVGIDTAH HAQAVMAAGF PQRMQKDYRD AIDALFDANR FGQKNGLGFW RYKEDSKGKP 600
KKEEDAAVED LLAEVSQPKR DFSEEEIIAR MMIPMVNEVV RCLEEGIIAT PAEADMALVY 660
GLGFPPFHGG AFRWLDTLGS AKYLDMAQQY QHLGPLYEVP EGLRNKARHN EPYYPPVEPA 720
RPVGDLKTA 729 
Gene Ontology
 GO:0003857; F:3-hydroxyacyl-CoA dehydrogenase activity; IEA:HAMAP.
 GO:0008692; F:3-hydroxybutyryl-CoA epimerase activity; IEA:HAMAP.
 GO:0050662; F:coenzyme binding; IEA:InterPro.
 GO:0004165; F:dodecenoyl-CoA delta-isomerase activity; IEA:HAMAP.
 GO:0004300; F:enoyl-CoA hydratase activity; IEA:HAMAP.
 GO:0006635; P:fatty acid beta-oxidation; IEA:UniProtKB-UniPathway. 
Interpro
 IPR006180; 3-OHacyl-CoA_DH_CS.
 IPR006176; 3-OHacyl-CoA_DH_NAD-bd.
 IPR006108; 3HC_DH_C.
 IPR008927; 6-PGluconate_DH_C-like.
 IPR001753; Crotonase_core_superfam.
 IPR013328; DH_multihelical.
 IPR018376; Enoyl-CoA_hyd/isom_CS.
 IPR012799; FadB.
 IPR016040; NAD(P)-bd_dom. 
Pfam
 PF00725; 3HCDH
 PF02737; 3HCDH_N
 PF00378; ECH 
SMART
  
PROSITE
 PS00067; 3HCDH
 PS00166; ENOYL_COA_HYDRATASE 
PRINTS