CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-020015
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Pre-mRNA-processing-splicing factor 8 
Protein Synonyms/Alias
 Splicing factor Prp8 
Gene Name
 Prpf8 
Gene Synonyms/Alias
 Prp8 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
43KWQQLQAKRYAEKRKubiquitination[1]
218QPLRDSRKYVNGSTYubiquitination[1]
267LKAFFTSKALNMAIPubiquitination[1]
278MAIPGGPKFEPLVRDubiquitination[1]
366ISHRHSVKSQEPLPDubiquitination[1]
428ALDIPLVKNWYREHCubiquitination[1]
702DMMPEGIKQNKARTIubiquitination[1]
746ILRYVKAKADWWTNTubiquitination[1]
1020NNVVINYKDMNHTNSubiquitination[1]
1158RAVFWDIKNRLPRSVubiquitination[1]
1210SYEEFTHKDGVWNLQubiquitination[1]
1300NKIQTRIKIGLNSKMubiquitination[1]
1344QSDLRWSKQTDVGITubiquitination[1]
1392VWAEYALKRQEAIAQubiquitination[1]
1425RINTLFQKDRHTLAYubiquitination[1]
1434RHTLAYDKGWRVRTDubiquitination[1]
1443WRVRTDFKQYQVLKQubiquitination[1]
1463THQRHDGKLWNLNNYacetylation[2]
1735QAMAKIMKANPALYVubiquitination[1]
1831TSVWAGQKRLGQLAKubiquitination[1]
1840LGQLAKWKTAEEVAAubiquitination[1]
1993LILADYGKKNNVNVAubiquitination[1]
2031QQIAEIEKQTKEQSQubiquitination[1]
2034AEIEKQTKEQSQLTAubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441] 
Functional Description
 Functions as a scaffold that mediates the ordered assembly of spliceosomal proteins and snRNAs. Required for the assembly of the U4/U6-U5 tri-snRNP complex. Functions as scaffold that positions spliceosomal U2, U5 and U6 snRNAs at splice sites on pre-mRNA substrates, so that splicing can occur. Interacts with both the 5' and the 3' splice site (By similarity). 
Sequence Annotation
 DOMAIN 2099 2233 MPN.
 REGION 812 1303 Reverse transcriptase homology domain.
 REGION 1304 1577 Linker.
 REGION 1513 1526 Important for branch point selection (By
 REGION 1581 1752 Restriction endonuclease homology domain.
 REGION 1669 2034 Involved in interaction with pre-mRNA 5'
 REGION 1767 2020 RNase H homology domain.
 REGION 2301 2335 Required for interaction with EFTUD2 and
 MOD_RES 2 2 N-acetylalanine (By similarity).
 MOD_RES 859 859 Phosphoserine (By similarity).
 MOD_RES 1318 1318 Phosphothreonine.
 MOD_RES 1463 1463 N6-acetyllysine (By similarity).
 MOD_RES 2042 2042 Phosphothreonine.  
Keyword
 Acetylation; Complete proteome; mRNA processing; mRNA splicing; Nucleus; Phosphoprotein; Reference proteome; Ribonucleoprotein; RNA-binding; Spliceosome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 2335 AA 
Protein Sequence
MAGVFPYRGP GNPVPGPLAP LPDYMSEEKL QEKARKWQQL QAKRYAEKRK FGFVDAQKED 60
MPPEHVRKII RDHGDMTNRK FRHDKRVYLG ALKYMPHAVL KLLENMPMPW EQIRDVPVLY 120
HITGAISFVN EIPWVIEPVY ISQWGSMWIM MRREKRDRRH FKRMRFPPFD DEEPPLDYAD 180
NILDVEPLEA IQLELDPEED APVLDWFYDH QPLRDSRKYV NGSTYQRWQF TLPMMSTLYR 240
LANQLLTDLV DDNYFYLFDL KAFFTSKALN MAIPGGPKFE PLVRDINLQD EDWNEFNDIN 300
KIIIRQPIRT EYKIAFPYLY NNLPHHVHLT WYHTPNVVFI KTEDPDLPAF YFDPLINPIS 360
HRHSVKSQEP LPDDDEEFEL PEFVEPFLKD TPLYTDNTAN GIALLWAPRP FNLRSGRTRR 420
ALDIPLVKNW YREHCPAGQP VKVRVSYQKL LKYYVLNALK HRPPKAQKKR YLFRSFKATK 480
FFQSTKLDWV EVGLQVCRQG YNMLNLLIHR KNLNYLHLDY NFNLKPVKTL TTKERKKSRF 540
GNAFHLCREV LRLTKLVVDS HVQYRLGNVD AFQLADGLQY IFAHVGQLTG MYRYKYKLMR 600
QIRMCKDLKH LIYYRFNTGP VGKGPGCGFW AAGWRVWLFF MRGITPLLER WLGNLLARQF 660
EGRHSKGVAK TVTKQRVESH FDLELRAAVM HDILDMMPEG IKQNKARTIL QHLSEAWRCW 720
KANIPWKVPG LPTPIENMIL RYVKAKADWW TNTAHYNRER IRRGATVDKT VCKKNLGRLT 780
RLYLKAEQER QHNYLKDGPY ITAEEAVAVY TTTVHWLESR RFSPIPFPPL SYKHDTKLLI 840
LALERLKEAY SVKSRLNQSQ REELGLIEQA YDNPHEALSR IKRHLLTQRA FKEVGIEFMD 900
LYSHLVPVYD VEPLEKITDA YLDQYLWYEA DKRRLFPPWI KPADTEPPPL LVYKWCQGIN 960
NLQDVWETSE GECNVMLESR FEKMYEKIDL TLLNRLLRLI VDHNIADYMT AKNNVVINYK 1020
DMNHTNSYGI IRGLQFASFI VQYYGLVMDL LVLGLHRASE MAGPPQMPND FLSFQDIATE 1080
AAHPIRLFCR YIDRIHIFFR FTADEARDLI QRYLTEHPDP NNENIVGYNN KKCWPRDARM 1140
RLMKHDVNLG RAVFWDIKNR LPRSVTTVQW ENSFVSVYSK DNPNLLFNMC GFECRILPKC 1200
RTSYEEFTHK DGVWNLQNEV TKERTAQCFL RVDDESMQRF HNRVRQILMA SGSTTFTKIV 1260
NKWNTALIGL MTYFREAVVN TQELLDLLVK CENKIQTRIK IGLNSKMPSR FPPVVFYTPK 1320
ELGGLGMLSM GHVLIPQSDL RWSKQTDVGI THFRSGMSHE EDQLIPNLYR YIQPWESEFI 1380
DSQRVWAEYA LKRQEAIAQN RRLTLEDLED SWDRGIPRIN TLFQKDRHTL AYDKGWRVRT 1440
DFKQYQVLKQ NPFWWTHQRH DGKLWNLNNY RTDMIQALGG VEGILEHTLF KGTYFPTWEG 1500
LFWEKASGFE ESMKWKKLTN AQRSGLNQIP NRRFTLWWSP TINRANVYVG FQVQLDLTGI 1560
FMHGKIPTLK ISLIQIFRAH LWQKIHESIV MDLCQVFDQE LDALEIETVQ KETIHPRKSY 1620
KMNSSCADIL LFASYKWNVS RPSLLADSKD VMDSTTTQKY WIDIQLRWGD YDSHDIERYA 1680
RAKFLDYTTD NMSIYPSPTG VLIAIDLAYN LHSAYGNWFP GSKPLIQQAM AKIMKANPAL 1740
YVLRERIRKG LQLYSSEPTE PYLSSQNYGE LFSNQIIWFV DDTNVYRVTI HKTFEGNLTT 1800
KPINGAIFIF NPRTGQLFLK IIHTSVWAGQ KRLGQLAKWK TAEEVAALIR SLPVEEQPKQ 1860
IIVTRKGMLD PLEVHLLDFP NIVIKGSELQ LPFQACLKVE KFGDLILKAT EPQMVLFNLY 1920
DDWLKTISSY TAFSRLILIL RALHVNNDRA KVILKPDKTT VTEPHHIWPT LTDEEWIKVE 1980
VQLKDLILAD YGKKNNVNVA SLTQSEIRDI ILGMEISAPS QQRQQIAEIE KQTKEQSQLT 2040
ATQTRTVNKH GDEIITSTTS NYETQTFSSK TEWRVRAISA ANLHLRTNHI YVSSDDIKET 2100
GYTYILPKNV LKKFICISDL RAQIAGYLYG VSPPDNPQVK EIRCIVMVPQ WGTHQTVHLP 2160
SQLPQHEYLK EMEPLGWIHT QPNESPQLSP QDVTTHAKIM ADNPSWDGEK TIIITCSFTP 2220
GSCTLTAYKL TPSGYEWGRQ NTDKGNNPKG YLPSHYERVQ MLLSDRFLGF FMVPAQSSWN 2280
YNFMGVRHDP NMKYELQLAN PKEFYHEVHR PSHFLNFALL QEGEVYSADR EDLYA 2335 
Gene Ontology
 GO:0071013; C:catalytic step 2 spliceosome; IEA:Compara.
 GO:0016607; C:nuclear speck; IEA:UniProtKB-SubCell.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0000398; P:mRNA splicing, via spliceosome; IEA:InterPro. 
Interpro
 IPR000555; JAB1_Mov34_MPN_PAD1.
 IPR012591; Pre-mRNA-splicing_factor-8.
 IPR012984; PRO_C.
 IPR012592; PROCN.
 IPR021983; PRP8_domainIV.
 IPR019581; Prp8_U5-snRNA-bd.
 IPR019580; Prp8_U6-snRNA-bd.
 IPR019582; RRM_spliceosomal_PrP8. 
Pfam
 PF01398; JAB
 PF08082; PRO8NT
 PF08083; PROCN
 PF08084; PROCT
 PF12134; PRP8_domainIV
 PF10598; RRM_4
 PF10597; U5_2-snRNA_bdg
 PF10596; U6-snRNA_bdg 
SMART
 SM00232; JAB_MPN 
PROSITE
  
PRINTS